| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,123,110 | G→A | 100% | P77L (CCA→CTA) | ftsN ← | cell division protein FtsN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,123,110 | 0 | G | A | 100.0% | 106.7 / ‑6.7 | 42 | P77L (CCA→CTA) | ftsN | cell division protein FtsN |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (18/22); minor base C (1/0); total (19/23) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACC > NC_000913/4122971‑4123247 | ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTGCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtg < 1:402940/143‑1 (MQ=255) tCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtgg > 1:610493/1‑143 (MQ=255) aCGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTc < 1:40347/143‑1 (MQ=255) aCGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTc < 1:400233/143‑1 (MQ=255) gttgttTTGGTGTCAGGTGCTCCGGCGTTTTCACTTCCCCGCCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGGCTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg < 2:99133/143‑1 (MQ=255) gttgttCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg < 1:577272/143‑1 (MQ=255) tCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCAGTTTCTgg > 1:305913/1‑143 (MQ=255) gTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCTAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCtt < 1:196028/143‑1 (MQ=255) ttGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCttt > 1:277160/1‑143 (MQ=255) tGCTCCGGCGTTTTCGCTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTg < 2:636924/143‑1 (MQ=255) ggCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTCCTTCTGGTTTTAGTGGTCGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGt > 2:495734/1‑143 (MQ=255) ttcacttcgcCGCCGGCAGAAGGTTCTGAGGTCGCACGACTTAGCTGATGCCGACTTTCCAGCTCTTTAATGTAGCGCGAGCGTTCTGCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGa < 2:556962/143‑1 (MQ=255) cttcacCGCCGGCAGAAGGTTCTGTGGGCGCACGCCCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCCTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGActct < 1:598249/143‑1 (MQ=255) accaccGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGTTCTGCAGCGTCTCCGACTcttctt > 1:265399/1‑143 (MQ=255) ccGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTtctttctt < 1:446012/143‑1 (MQ=255) ggctgcaGGTTCTGTGGGCGCCCGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCTCTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtgt < 2:425634/137‑1 (MQ=255) caGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGc < 1:33533/142‑1 (MQ=255) ttCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTaa > 1:518996/1‑143 (MQ=255) gtgGGCTCTCCCTCTCCGTGCTGGCGGCTTTCCAGATCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTACTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGaa < 2:97038/143‑1 (MQ=255) gtcggCGCACGCACTCCCGGCTTGCGACTGTCCAGCTCTTTGGTGTTGCTACCTCCCCCTTCTGGTTTGAGACGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGaa < 2:629156/140‑1 (MQ=255) gggggCGCATGCTCTCCCGGCTGGCGGCGTGCGAGCTCTTTACTGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTGTGGCTTTGCTGCGTCTCGGACTCTTCTTTCTGGTGATGCGTAATGaa < 2:460580/141‑1 (MQ=255) gggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGCTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTCGCTTTGCAGCGTCTCGGACTCTTCTCTCTTGTGATGCGTAATGAAGt > 2:467869/1‑143 (MQ=255) ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGGGATATGTTAATGTTATTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTa < 1:470846/143‑1 (MQ=255) ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTACTAAAGTa > 1:203426/1‑143 (MQ=255) ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAACGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCCCTTTCTGCGGTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTTATgtagta > 2:139312/1‑143 (MQ=255) gcacgcacTCCCGGGTGGCGACTTTGCAGCTCTTTAAGGGAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAg < 1:45775/143‑1 (MQ=255) cacgcacTCCCTGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTCTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGa < 2:321895/143‑1 (MQ=255) acgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCCGCGGTCTTCTGGTTTTCGTGTTAGTCCGTCTCCGTTCGCTTCCTGGCTTTGCAGCGTCTCGGACTCTCCTTTCCTGTGTTGCGCAATGAAGTAccgcc > 2:59008/1‑139 (MQ=255) cccTCCCGGCTGGCGACTTTCCCGCTCTTTGATGGAGCGCCAGCGTTCTTCTGGTTTTACTGCTAGTCCGTGTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGCCTCTTCTTTCTTGGGATGCGTAATGAAGTACAGaccac < 2:524257/141‑1 (MQ=255) acTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTATCGaccccc > 1:480469/1‑143 (MQ=255) gtttgctaCTTTTCCGCTCTTTAATGTTGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAg < 2:570005/136‑1 (MQ=255) cTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGCCTCTTCCTTCTTGTGATGCGTAATGAAGTACAGACCACCGATACAgg > 2:403579/1‑143 (MQ=255) ttaccAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGCTTTAGTGGTAGTGCGTTTCCGGTCACTTTCTGGCTTTGGAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGa > 2:618912/4‑143 (MQ=255) tctaGCTTTTTAATGTAGTGCCAGCGTTCTTCTGGTTTTATTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTTGGACTCTTCTTTCTTGTGTTGCGTAATGATGTACAGACCACCGATAAAGGTCACAAGAAc < 2:137314/140‑1 (MQ=255) aGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc > 1:102283/1‑143 (MQ=255) gTAGCGCCAGCGTTCTTCTGGACTTAGTGGTAGTCAGTTTCCGGTCACTTTCTGTCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGCTGCGTACTGAAGTACAGCCCACCGCTAAAGGCCACTGGACCGCCGgcggcacccg > 2:235153/1‑139 (MQ=255) tAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGc > 1:405116/1‑143 (MQ=255) gcgcGAGCGTTGTTCTGGTGTTAGTGGTAGTCCGTTTCCGGTCCGTTGCGGGCTTTGCAGCGTGTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGa < 2:204990/143‑1 (MQ=255) gcgcCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCCCCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGa > 2:489092/1‑143 (MQ=255) ccAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCCCTTTCTGGCTTTGCAGCGTCTCGGTCTCTTCCTTTTTGTGATGCGTGATGAAGTACAGACCACGGGTAAAGGTCACACGTGCGGGGGCAGCAATGGGGACCa > 2:585536/1‑143 (MQ=255) cGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGAc > 2:600843/1‑137 (MQ=255) cGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGAc < 1:600843/137‑1 (MQ=255) gtcttcaGGTCTTTGTGTTAGTCCGTTTCCTGTTCCTTTCTGGCGATTCAGCGTCTCGGACTCTTGGTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCgg < 2:129456/142‑1 (MQ=255) gTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAGAGGTCACAGGAACGGCGGCAGCAATAGCGACCGTAGCGGGAGAAGcc > 1:3845/1‑143 (MQ=255) | TTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACC > NC_000913/4122971‑4123247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |