| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,184,543 | C→A | 100% | P1100Q (CCG→CAG) | rpoB → | RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0% | 103.6 / NA | 34 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/20); total (14/20) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCG > NC_000913/4184405‑4184671 | tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg > 2:412517/1‑143 (MQ=255) cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACCCCGGTCGACAACGTACTGAACCAGCTGGGc > 2:206708/1‑143 (MQ=255) ccGTATCCAGCCTGGTGACGAGATGGCAGGTCGTCACGGTAATAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc < 2:202503/143‑1 (MQ=255) gTAGCCAGCCGGGTGACACGATGGCGGGTCGTCACGGTAAGAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt < 2:620884/143‑1 (MQ=255) tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg > 1:613513/1‑143 (MQ=255) ccggCCTCGTGACAAGACGGCAGGCCGTCACGGTAACAAGGGTGTAATTTCGAAGATCAACCCGATCGAAGCTATGCCCTACAATGAAAACGCTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa < 2:107042/140‑1 (MQ=255) ccAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa < 1:38850/143‑1 (MQ=255) cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa > 1:557382/1‑143 (MQ=255) aGCCTGGTAACATGGTGGCAGGTCTGCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAc < 2:22736/143‑1 (MQ=255) aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAACATCAACCCGATCGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACAGCGTACTGCACCAGTTGGGCTCACCTACTCGTATGACGAGCTGTCAGATCCt < 2:462055/143‑1 (MQ=255) aGGTCATCACGGTAACAAGAGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa < 1:254408/143‑1 (MQ=255) gcgggaCAAGGCTGTAAGTTCTAAGCGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTATACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg < 2:587458/138‑1 (MQ=255) caaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTa < 2:375876/142‑1 (MQ=255) aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGc > 1:421439/1‑143 (MQ=255) gggTGTAATTTCCAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTg < 2:507483/143‑1 (MQ=255) tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAAGAACATCGGCCGGAGCCGCGAACCCCACCGGGACAG‑GGCTggca > 2:570269/1‑140 (MQ=255) tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGaaa > 1:459322/1‑143 (MQ=255) tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGCTCCTCGCAACCCACCTGGGTAT‑GGCTGCGAAAGGTAGCg > 2:149784/1‑143 (MQ=255) aaGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATGGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCGAAACACACCAGGGTAT‑GGCTCCGAAAGGTAACgg > 2:124220/1‑143 (MQ=255) gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTAgcggcg > 2:533402/1‑143 (MQ=255) aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTACCGCCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCCGCGCAC‑GACTGCGAAACGCAGCGGCGa > 2:331821/1‑143 (MQ=255) tCGAAGCTATGCCCTACGCTGAAACCGGTACGCCGGGAGACATCGTACTGAACCAGCTGTGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAAc < 2:614251/143‑1 (MQ=255) gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGc < 2:609259/143‑1 (MQ=255) aaGATATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGcc < 1:545902/143‑1 (MQ=255) gtatGCCTTACGATGGTGCCGGTACGCGGGTAGACATCGTACTGCACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg < 2:612572/142‑1 (MQ=255) gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa < 1:539147/143‑1 (MQ=255) ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGGAAGGTATAGGGGACGAGATCAACGCCATGCTGaacc > 2:30842/1‑141 (MQ=255) tGAAAACGGTACGCCGGTAGACCGCGTACTGCACCAGCTAGTCTTACCGTCTCGTATGCACATACGTAATAATCTCGACGGTCTTC‑GGGTCTACGCCGCGAAAGTTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc < 1:299033/143‑1 (MQ=255) agAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc < 1:298170/142‑1 (MQ=255) gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACCgcagca > 1:193853/1‑143 (MQ=255) gaCGCGGGAAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg < 2:441038/142‑1 (MQ=255) aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc < 1:97119/143‑1 (MQ=255) aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc < 2:17297/143‑1 (MQ=255) cATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTGT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCCGCAAGAAGTCGCGACACTGcgcg > 1:498187/1‑143 (MQ=255) | TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCG > NC_000913/4184405‑4184671 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |