| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 696,470 | C→T | 100% | noncoding (35/75 nt) | glnX ← | tRNA‑Gln |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 696,470 | 0 | C | T | 100.0% | 87.2 / ‑6.7 | 32 | noncoding (35/75 nt) | glnX | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (18/13); total (19/13) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAG > NC_000913/696338‑696584 | ccGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCAGGAATTTGAATCCGGt > 1:398808/1‑143 (MQ=255) cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGGACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg > 1:41523/1‑143 (MQ=255) gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc > 1:79593/1‑143 (MQ=255) tGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGTATGCCGGAATTAGGATCCGGTGCCTTAc > 2:374373/1‑143 (MQ=255) tGAAGGATAAGACGTGGCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAGGCCCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc < 2:225425/143‑1 (MQ=255) gtgtCAACATCGCATGCGACATTTAAGGAACGCAGACAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTACTGTGGCTGCGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAc < 2:612364/143‑1 (MQ=25) gtCAACATCGCATTCGACATTGAATGAACGCGGAAAAGCAAAAAGCTCGCCGACGCGAGCGTTTTTAATGTGGCTGGGGCACGAGGATTCGAACCTCGGAAAGCCCGAGTCAGAATCCGGTGCCTTACCGCTGGGCGCTGccc > 2:429995/1‑143 (MQ=25) tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg < 1:485394/143‑1 (MQ=255) cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg > 1:320188/1‑143 (MQ=255) gCATTCGACCGTGAATGAACGCAGAAAAGGAAAAAGCTCGCCGAAGCGAGCTTTTGTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAGCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt < 2:117867/143‑1 (MQ=21) gCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt < 2:124055/143‑1 (MQ=255) cATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACGGGGTg > 1:135674/1‑143 (MQ=255) gACATTGAATGTACGCACAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACtt < 1:163408/143‑1 (MQ=255) ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa > 1:415442/1‑143 (MQ=255) aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCAAACTGGGTGCACTTACAAGGTAAGCGTCtt < 2:79593/143‑1 (MQ=255) aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa > 1:20033/1‑143 (MQ=255) cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGCATCCGGTGCCTTACTGCTTGGCGATACCCCAACTGCGTGCACTTACCAGTTAACCGCATAGAATaaa > 2:373593/1‑143 (MQ=21) cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa > 1:444361/1‑143 (MQ=255) cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGCCGATACCCCAACTGGGTGCTCTTACAACGTCACCACCTTGAATaca > 2:88085/1‑141 (MQ=21) aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt < 1:586334/143‑1 (MQ=255) aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt < 2:415442/143‑1 (MQ=255) tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg > 1:40993/1‑143 (MQ=255) aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTGCAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa > 1:562357/1‑143 (MQ=255) gAGCTGTTAGAACGTGGCTGGGGCACGAGGATTCGAACCCCGGAATGCCGGAATTAGAGTCCGGGGCCTTACCGCTTGGCGCTACCCCAACTGGCTGCACTGATAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGa < 1:374373/143‑1 (MQ=255) ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTGCAAGGTAAGCGTATTGAATAAATTGGCTGGGGTACGAGGATTCg > 1:468233/1‑143 (MQ=255) ttttAATGTGGCTGGTGTACGAGGATTCGAAGTTCGGAATGCCGTAATTAGAATCCGGTGCATTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa < 1:341765/143‑1 (MQ=37) ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATTCGGTGCCTTACCGCTGGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa < 1:341242/143‑1 (MQ=255) agtcaaTGAGGCTGGGGTACATGGTGTCGAGCCACGGAATGCCGAAATTAGAATCCATTGCCTTAGCGCTTGGCGATACCCCAACTGGGTGCAATCACGAGGTAACCGGCTTGAATAAAGTGGCTGGGGTACGAGGATTCGaa < 2:353128/139‑1 (MQ=255) tatAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAACCGGTGCCTTACCTCTTGGCGATACCCCAACTGGGTGCACTTAGACGGTAGGCGGCCTGAATAAATAGGATGGGGTACGAGGATTAGAAc > 1:287840/3‑143 (MQ=18) tactgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCGTGGCGATACCCCAACTGGGTGCATTTACATGGGACGCGTCTAGAATAAATAGGCGGGGGTACGCGGCTTCGacgcg > 2:619331/4‑139 (MQ=17) tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGGTTCGAACCTCgg > 1:299675/1‑143 (MQ=255) gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTGCAAGTTAAGCATCTAGAATAAATTGGCAGGCGTACGCGGCTTCGACCCTCGTAGAGCCTGAATccg > 2:4898/1‑141 (MQ=18) | CCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAG > NC_000913/696338‑696584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |