Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 709,461 C→T 9.2% G376G (GGC→GGT)  chiP → chitobiose outer membrane channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913709,4610CT 9.2% 106.8 / 2.5 44G376G (GGC→GGT) chiPchitobiose outer membrane channel
Reads supporting (aligned to +/- strand):  ref base C (19/21);  new base T (2/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

CTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAG  >  NC_000913/709319‑709603
                                                                                                                                              |                                                                                                                                              
cTCCAACCTACGCTTGCTCAACCGCTCGCCTTGATATCTGGTGGGATCGCCGTTTTGACTTGAACGCCAACGGCGAAAAAGCGGTCTTCTGCGGTGTGATGTTGGAGCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGgc                                                                                                                                                <  2:44941/143‑1 (MQ=255)
cTCCAACCTACGCTTCCTCGAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCTACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGgc                                                                                                                                                <  2:349608/143‑1 (MQ=255)
 tCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCACCGgcg                                                                                                                                               >  2:317433/1‑143 (MQ=255)
      ccTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTcc                                                                                                                                          >  2:602736/1‑143 (MQ=255)
            cTTCCTCAAACTGGCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGtt                                                                                                                                    >  2:111660/1‑143 (MQ=255)
                 tCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGTGCTTCCTACGTTTACGc                                                                                                                               >  1:587440/1‑143 (MQ=255)
                 tCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGTGCTTCCTACGTTTACGc                                                                                                                               >  1:585696/1‑143 (MQ=255)
                 tCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGc                                                                                                                               >  2:526690/1‑143 (MQ=255)
                       ggTCGCCTGGATATCTGGTGGGATAACCGGTCTGACTTCAACGCCAACGGCGAACTAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGa                                                                                                                         <  1:540318/143‑1 (MQ=255)
                             cTGGATATCTGGTGGGATAACCGTTCTGACTTCACCGCCAACGGCGATAAAGCGTTCTTCTTCATTACGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTAGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTaa                                                                                                                   >  1:85706/1‑143 (MQ=255)
                             cTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGCTGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGAGGCTaa                                                                                                                   >  1:164120/1‑143 (MQ=255)
                             cTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTaa                                                                                                                   <  1:512495/143‑1 (MQ=255)
                               ggATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAAc                                                                                                                 <  1:97689/143‑1 (MQ=255)
                               ggAGATCTGGTGGGATGACCGTTCTGACTGCGACGCCAACGGCGGAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAAc                                                                                                                 <  2:240371/143‑1 (MQ=255)
                                 atatCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCt                                                                                                               >  1:535441/1‑143 (MQ=255)
                                     cTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGa                                                                                                           <  2:644974/143‑1 (MQ=255)
                                         tggGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTg                                                                                                       >  1:191567/1‑143 (MQ=255)
                                           ggATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGc                                                                                                     >  1:63072/1‑143 (MQ=255)
                                            gATAAGTGTTCTGACTTCAACGCGAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGTGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCa                                                                                                    <  2:585696/143‑1 (MQ=255)
                                            gATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGTGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCa                                                                                                    <  2:587440/143‑1 (MQ=255)
                                                    ttCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGAGATGCTAAACCTGCGACCTGGAAGAGCAATc                                                                                            >  1:183304/1‑143 (MQ=255)
                                                               aCGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGtac                                                                                 <  1:602736/143‑1 (MQ=255)
                                                                    aaCGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGa                                                                            <  1:253977/143‑1 (MQ=255)
                                                                                 cGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACt                                                               >  2:344807/1‑143 (MQ=255)
                                                                                     cttcttCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTg                                                           >  2:165407/1‑143 (MQ=255)
                                                                                       tcttctGTTCTATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGTCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGaa                                                         <  1:17380/143‑1 (MQ=255)
                                                                                               gCGATGTAAGACCTGAAAAACTGGAATCTCCCAAGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGc                                                 <  2:307564/143‑1 (MQ=255)
                                                                                                  aTGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAaga                                                       <  2:268826/134‑1 (MQ=255)
                                                                                                  aTGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAaga                                                       >  1:268826/1‑134 (MQ=255)
                                                                                                     tATGACCTGAAAAACTGGAATCTTCCAGGCTTCCCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAg                                           <  1:274905/143‑1 (MQ=255)
                                                                                                     tATGACCCGAAAAACTGGAGTCTTCGAGGCTGCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAg                                           <  1:575119/143‑1 (MQ=255)
                                                                                                      aTGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGc                                          <  1:384289/143‑1 (MQ=255)
                                                                                                         aCCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGACTGCCTACAGCCTg                                       >  1:635657/1‑143 (MQ=255)
                                                                                                          ccTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTgg                                      >  1:229642/1‑143 (MQ=255)
                                                                                                            tGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGACACGCCTGCATACAGCCCGGac                                    >  2:251265/1‑142 (MQ=255)
                                                                                                                aaaCTGGAAGCTTCCAGGCTTCGCCATCGGCGCGTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCgg                                <  2:9294/143‑1 (MQ=255)
                                                                                                                 caCTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGt                               <  1:126610/142‑1 (MQ=255)
                                                                                                                 aaCTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCCTGGGATGCTAAACCTGCGACCTGGCCGAGCAATCCGGATGCGTACTACCACAAAAACCGGCCCATTGACGAGGCTGCCCACCGCCCGGAAGCGGt                               >  2:121066/1‑143 (MQ=255)
                                                                                                                    tGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTCTTGAAGAGTCTGCCTACAGCCTGGCTGCGGGCtg                            >  1:1366/1‑142 (MQ=255)
                                                                                                                    tGGAATCTTCCAGCCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCta                            <  1:122243/143‑1 (MQ=255)
                                                                                                                        aTCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGCGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATAcc                        <  1:526690/143‑1 (MQ=255)
                                                                                                                           ttCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCAtt                     <  1:317433/143‑1 (MQ=255)
                                                                                                                               aGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAgg                 >  1:27370/1‑143 (MQ=255)
                                                                                                                                 tcTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGAc               <  2:215374/142‑1 (MQ=255)
                                                                                                                                     ggtcATCGGCGCTTCTTACGTTTACGCATTGGATTCTAAACCTGCGACCTGGCAGAGCAATCCTGATGCGTACTACTACAAAAACCGGACTATTGAAGAGTCTGCCTACATCCTGGATGCGGTCTATACCATTCAGGACGGTc           <  2:183304/140‑1 (MQ=255)
                                                                                                                                       ccAGCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCGGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTcgc         <  1:62609/143‑1 (MQ=255)
                                                                                                                                          tCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACCAACACCGGACTATTGCAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATACACGACGGTCGCGcc      >  2:491581/1‑143 (MQ=255)
                                                                                                                                              cgcTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTCCGATAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGCCGGTCGCGCGaacg  >  2:389702/1‑141 (MQ=255)
                                                                                                                                              cgcTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAACACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATACGGACTATACCATTCAGGACGGTCGTGCCACAg  >  2:132044/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAG  >  NC_000913/709319‑709603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑