| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 95.9% | 131.5 / NA | 49 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (25/22); minor base G (0/2); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAA > NC_000913/125245‑125526 | tGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGGAACGCCGCGCGTTCCGTATAGCGCTc > 2:811622/1‑143 (MQ=255) tCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtg < 1:812775/143‑1 (MQ=255) cTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGAAGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtgat > 1:112631/1‑143 (MQ=255) tGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtgatg > 1:686206/1‑143 (MQ=255) ggCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGAAACATGCTGCACCCGCTGGACACTCCGCGCGTTCGGTATATCGCTTCGGtgataa > 1:313401/1‑141 (MQ=255) gCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGaa > 1:643663/1‑143 (MQ=255) aGATTAGGGCGTGGGGTCTGACTGTTATAGGGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacgacg < 1:1041604/143‑1 (MQ=255) aGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCCCGTAGGTGATGAacgacg > 1:566377/1‑143 (MQ=255) ttccGGCGTATGTTCTGACGTTTATTGTGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTAAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAATTCCGCGCTTTCCGTATATCGCTTAGGTGATGAACGACGCTc < 1:794437/140‑1 (MQ=255) ttACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTc > 1:612050/1‑143 (MQ=255) tACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTcc < 1:757410/143‑1 (MQ=255) gCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCAGCa > 1:32386/1‑143 (MQ=255) gTAGGTTCTGCCGTTTCTAGCGTGTCCTCCTTCTCCGTGCTGGCGTGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGt < 2:105793/143‑1 (MQ=255) aagtacTGACGTTTATAGCGCGACCTCCTTCACCGAGCTGGCGCGTTATGGTCACGATTGTGAACGCTGGAACATGCCGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTgg < 1:467939/138‑1 (MQ=255) tCTGTCTTTTATTGCGTGATCTCCTTCTCCGGGCTGGCGGGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATc < 2:904985/143‑1 (MQ=255) gACGTTTATAGCGGGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCACCACCAAAGGTTCTTTGGTGATGAACGACGCTCCGGCAGTGGCATCTAc < 1:795208/143‑1 (MQ=255) aCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAcc > 1:1028488/1‑143 (MQ=255) aCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGGCAGGATGGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGCCGCTCCGCCAGACGCATCTAcc > 2:157718/1‑143 (MQ=255) ataGCGTGACCTCCTTCACCGAGCTGGCGCGTCATGGTAATGACTCTTAGCGCTGGCCCCTATGACACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCGTAGGGGATTAACGACGCTCCGGCAGTGGCATCTACCGACtat < 1:1031819/143‑1 (MQ=255) ataGCGGGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACtat < 1:91206/143‑1 (MQ=255) cGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGCTTGTGAACGCTGGAACCTGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCGGCGGAATCTACCGACGATAGGa > 1:394260/1‑143 (MQ=255) cGTGACCTCCTGGATCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGa < 2:739899/143‑1 (MQ=255) tgcggtccgTCAACGAGCTGGCGCGTGATGGTCAGGATTGTGAGCGCTGGAAGATGCTGCACCCGCGGGAAACTCCGCGCGGTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGaaa < 2:232511/138‑1 (MQ=255) tgcccccctTGACCCAGCTGGCGCGTGATGGGCAGGATTGGGAAGGGTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGaaa < 1:1128622/137‑1 (MQ=255) cctcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTg > 1:198241/1‑143 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGtt > 1:169532/1‑143 (MQ=255) aCCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGa < 1:508056/143‑1 (MQ=255) ccGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGCTGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTGCGCTgcg > 2:325041/1‑141 (MQ=255) ggctgtggcgTGAGGGTCAGGGTGGTGAACGCTGGAACATGCTGGAACGGCGGTGAGCTTCGCGCCTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAg < 2:513058/136‑1 (MQ=255) cTGGCGCGTGATGCCAACGCTAGTGTCCGCTGCAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTTTATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGt < 2:1059000/143‑1 (MQ=255) tGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATCTCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTc > 2:834324/1‑143 (MQ=255) cggCGCGTGAGGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTc < 2:391707/142‑1 (MQ=255) gTGATGGTCAGGATTGTGAACGCGTGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTGAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACt < 2:784661/143‑1 (MQ=255) gATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTa < 2:686206/143‑1 (MQ=255) gTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTCATTACGTa > 1:150995/1‑143 (MQ=255) cAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGGACGACGCTCCGGCAGTGGCATCTACCGACTATATGCAACTGTTCGCTTAGCAGGTCCGTTCTTAcctccc > 2:709296/1‑138 (MQ=255) gATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGGTCCGTATATCGCTTGGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCGGGTCCGTACTCACGTACCGGc > 1:21598/1‑143 (MQ=255) aaCGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGCTGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGCGCAGGTCCGTACTTACGTCCCGGCTgacgac > 1:232798/1‑143 (MQ=255) gctgcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGCCGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCGGTCCTTACGTACCGGCTGACGAGTACCGCCTACTgg > 1:1028821/1‑143 (MQ=255) gctgcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTGAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTgg < 2:198063/143‑1 (MQ=255) cggctcgcGCTGGACATGCCGTGGGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTACTCGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTggg < 2:392340/136‑1 (MQ=255) gcACCCGCTGGAAACTCCGCGCGTCCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGAATATAAGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTa < 2:1071554/143‑1 (MQ=255) cgcgTTCCGTATATCGCTTAGGTGATGAACGACGCCCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTc < 1:171052/143‑1 (MQ=255) ttCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTGCGTTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTGCTGGGTACTGATGGCTTCGGTCGTTGCggc > 1:1037575/1‑141 (MQ=255) tCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTGCGGCa > 1:51733/1‑143 (MQ=255) atatCGCCTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGCGTATGTGAAACTGTTCGCTTAGCGGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGt < 1:1095933/143‑1 (MQ=255) atCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAACAGGTCCGTACTTACGTACCGGCTGACGCATACCGCGTACTGGGTAATGATGGCATCGGTCGTTCCGACAGCCCTga > 2:110264/1‑143 (MQ=255) tCGCTTGGGTGATGAACGAAGCTCCGGCAGTGGCAACTACCGACTCTATGAAACTGTTCGCTGAGCGGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGATACTGATGGCATCGGTCGTTCTGACAGCCATgag > 2:108743/1‑143 (MQ=255) tCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCGGAGAGCCGTgag > 1:539193/1‑143 (MQ=255) tCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACCGCCGTgcg > 2:406882/1‑141 (MQ=255) gcgcAGGGGACGAACGCCGCTGCGGCAGTGGCATCGACCGACTATAGGAAACTGTTCGCTGAGCAGGTCCGTACTGACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGaa < 2:937545/140‑1 (MQ=255) | TGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAA > NC_000913/125245‑125526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |