Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,934,862	G→A	R485C (CGT→TGT)	zwf ←	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,934,862	0	G	A	100.0%	141.2 / NA	47	R485C (CGT→TGT)	zwf	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/20);  total (27/20)

TAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCG  >  NC_000913/1934723‑1935000                                                                                                                                            |                                                                                                                                           gaataaAAATAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAAcc                                                                                                                                         <  2:591344/142‑1 (MQ=255)    taaAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATc                                                                                                                                      >  1:973182/1‑143 (MQ=255)    taaAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTCTTCTACAATCTGCGCAAGACCCTGTTACGGGTAAAATAACCCTAACGGACAAGCGCACATATTCCGCAAACCCACTCCAGGAACAACCacc                                                                                                                                      >  2:357828/1‑141 (MQ=255)     aaaaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGTAACAACCATCa                                                                                                                                     >  1:894155/1‑143 (MQ=255)              cTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAatc                                                                                                                            <  1:362170/143‑1 (MQ=255)                cGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAatcat                                                                                                                          <  2:215935/143‑1 (MQ=255)                 gTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAatcatc                                                                                                                         <  1:903228/143‑1 (MQ=255)                          tCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCCTTCCTCAAACAACCATCACGGGTAATCATCGCCACCGAg                                                                                                                <  1:274139/143‑1 (MQ=255)                             gCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCa                                                                                                             <  2:854698/143‑1 (MQ=255)                                  aGGCCTTAAATTGTAAAACGTGTTATACAATCTGGTCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCTCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCACCGCCACCGAGGCAACggg                                                                                                        <  2:570695/143‑1 (MQ=255)                                       tGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc                                                                                                   <  1:447552/143‑1 (MQ=255)                                       tGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCAGCACGGGTAATCCTCGCCACCGAGGCAACGGGGcccc                                                                                                   >  1:720578/1‑143 (MQ=255)                                          aaGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTAACGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAAACATCACGGGTAATCATCGCCACCGAGACAACGAGACCCCCgg                                                                                                >  2:478918/1‑143 (MQ=255)                                              gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTGCTCAAACTCATTCCAGGAACAACCATCACGGGTAATCAGCGCCACCGAGACAGCGGGTCCCCAGGGTcc                                                                                            >  1:105853/1‑143 (MQ=255)                                                 aaaaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGATCCCCAGGTTCCGGc                                                                                         >  1:436723/1‑143 (MQ=255)                                                           tACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGACAAGCGCAGATATTACTCAAACTCCTTCCAGGAACAACCATCACGGGTAATCATCGCCACCGACGCAACGGGCCCCCAGCATCCGGCCTGacccggc                                                                               >  2:612676/1‑137 (MQ=255)                                                            aCAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCATATATGACTCAAACTGACACCAGAAACAACCATCACGGGTAATCATCGCCGCCGAGCCAACGGGTCCCAAGGTTCCGGCCTGACACgggt                                                                              >  2:138915/1‑141 (MQ=255)                                                             cAATCTTCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGttt                                                                             >  2:885201/1‑143 (MQ=255)                                                                tCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCACCACGGGTACTCATCGCCACCGAGGCAACGGGCCCCGAGCATCCGGCCTGATCCgggtccgc                                                                          >  2:12791/1‑137 (MQ=255)                                                                tCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATCCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg                                                                          <  1:335507/143‑1 (MQ=255)                                                                   gcgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcgc                                                                       <  2:245789/143‑1 (MQ=255)                                                                     gcAAGATCATGTTACCGGTAAAATAACCATAAAGGACAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTACGGCCTGATACGGTGTCGGCGcag                                                                     >  1:746889/1‑142 (MQ=255)                                                                          aTCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTg                                                                <  1:1118023/143‑1 (MQ=255)                                                                            cATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTc                                                              >  1:22614/1‑143 (MQ=255)                                                                            cATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTc                                                              <  1:765873/143‑1 (MQ=255)                                                                            cATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTc                                                              >  1:493662/1‑143 (MQ=255)                                                                            cATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTc                                                              <  2:640957/143‑1 (MQ=255)                                                                                  aCCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGc                                                        <  1:283862/143‑1 (MQ=255)                                                                                  aCCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGCTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGACCCGGTTTCCGCGCACGATTTTCCATCGc                                                        >  2:824161/1‑143 (MQ=255)                                                                                  aCCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGCAACAACCATCACGGGTAATCATCGCCCCCCAGGCAACGGGGCCCCAGGGTCCGCCCTGCCACAGGTACGGCGCCTCACTATcccccgc                                                        >  2:342643/1‑138 (MQ=255)                                                                                      gTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGACACCGTTTCGGCGCATCCATGTCCATCGccccc                                                    >  2:1155156/1‑141 (MQ=255)                                                                                           aTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGCGTCCCCAGGCTCCGGCCAGTTTTAATTTCCGCCCACCCTTGCCCATCGCCCCCGCCTc                                               >  2:1120623/1‑143 (MQ=255)                                                                                            tAACCAGAACGGATAAGCGCAGATATTACCCAATCTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCa                                              <  1:279494/143‑1 (MQ=255)                                                                                                 aTAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGACGCAACGGGCCCCCAGGATCCGCCCTGATACGGTTTCGACGCGTCAGCGTCCCTCGCCCACGCCCCAggagc                                         >  2:623101/1‑139 (MQ=255)                                                                                                  tAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCCCGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATg                                        <  1:623087/143‑1 (MQ=255)                                                                                                  tAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATg                                        >  1:912126/1‑143 (MQ=255)                                                                                                  tAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATg                                        <  1:506384/143‑1 (MQ=255)                                                                                                   aaaGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGCGCCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCCTTGTCCACCGCCCACGCCCCAGTaacgc                                       >  2:116442/1‑140 (MQ=255)                                                                                                             cgcAGATATTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCa                             >  1:370702/1‑143 (MQ=255)                                                                                                                  atatTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAGGGAGTCTACCCATTTcc                        >  1:323016/1‑143 (MQ=255)                                                                                                                       aCTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTACTGGAGTCTACCCCTTTCCAGGct                   >  1:104466/1‑143 (MQ=255)                                                                                                                        cTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCAGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGctt                  <  1:905916/143‑1 (MQ=255)                                                                                                                         tCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGCGTCTACCCATTTCCATGcttg                 >  1:582383/1‑142 (MQ=255)                                                                                                                         tCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGcttc                 >  1:821021/1‑143 (MQ=255)                                                                                                                                cATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACt          <  1:885201/143‑1 (MQ=255)                                                                                                                                  ttCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTtc        >  1:883200/1‑143 (MQ=255)                                                                                                                                        gAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTGAGTAATGGCGTATCCCAAGATTCAACGGTCTGCCACTTCGTcgcg  <  1:1155156/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                           TAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCG  >  NC_000913/1934723‑1935000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑