Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,632	T→A	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	100.0%	192.2 / NA	64	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (31/33);  total (31/33)

TGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTA  >  NC_000913/3041499‑3041768                                                                                                                                      |                                                                                                                                         tGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcgc                                                                                                                                 <  1:1008756/143‑1 (MQ=255)   cGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCgcgc                                                                                                                               >  1:203894/1‑143 (MQ=255)          aaaGGTAAAATGTGGAGCAATTTACGTCTGGTCCGCGAAGGAGATGAATTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacga                                                                                                                        >  1:733522/1‑143 (MQ=255)               gaaaaTGTGGAGCAATTTCCGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGc                                                                                                                   <  1:413316/142‑1 (MQ=255)                    tgtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGtgtg                                                                                                              <  1:624423/143‑1 (MQ=255)                       ggAGCAATTTACGGCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgctg                                                                                                           <  1:1024872/143‑1 (MQ=255)                        gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgctgc                                                                                                          >  1:764200/1‑143 (MQ=255)                         aGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAGATATGCGGTATTCTCTAAAGAGACCATCGCGACAGACGACGAGCGTGTgctgct                                                                                                         >  1:241293/1‑143 (MQ=255)                          gCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtgtcccgcgt                                                                                                        >  2:62768/1‑136 (MQ=255)                          gCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCtt                                                                                                        <  1:1135709/143‑1 (MQ=255)                           cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGGATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCGGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                       <  1:228858/143‑1 (MQ=255)                           cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                       <  2:30038/143‑1 (MQ=255)                                 aCGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTg                                                                                                 <  1:650449/143‑1 (MQ=255)                                  cGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                                                                >  2:462017/1‑143 (MQ=255)                                   gTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTCAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGcc                                                                                               >  1:508327/1‑143 (MQ=255)                                    tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                              >  1:365273/1‑143 (MQ=255)                                    tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                              >  1:747330/1‑143 (MQ=255)                                           cgcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                                                       <  1:1122685/143‑1 (MQ=255)                                            gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                      <  1:632206/143‑1 (MQ=255)                                            gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                      <  1:860903/143‑1 (MQ=255)                                                    gacggCTCTGCATGGATTGAACGGCGCAGCGGGCGTGAACCGCAGCTGCATGACCTGAAAAAATATCCGGTATTCTCGACAGAGACCATCGCGCCAGACGTCGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                                                              <  1:373065/140‑1 (MQ=255)                                                    gATGGCTTTGCATGGATTGAACGGCACAGCGTGCGTGAACCGAAGCTGACTGAACTGAAAAAATATGCAGAATTCTCTAACGAGACCATCGCGCCAGACGACGCGCGTGTGCTGCTCGGTCTGTCCGGTTTCCAGgagcgcgc                                                                              >  2:836425/1‑143 (MQ=255)                                                        gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg                                                                          <  1:680350/143‑1 (MQ=255)                                                          tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCCCGCt                                                                        <  1:325232/143‑1 (MQ=255)                                                          tttGCATGGATTGAACGGCAAAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGAGGAGCGTGTGCAGATTGGTGTTACCGGTATCAAGGCGCGCGCCGGGCt                                                                        >  2:227148/1‑143 (MQ=255)                                                               aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa                                                                   >  1:828680/1‑143 (MQ=255)                                                               aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCAGCTTGGTGTTGCCGGATAGCAGGCGCGCGCCGCGCCGGCaa                                                                   >  2:858230/1‑143 (MQ=255)                                                               aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATGCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa                                                                   >  1:593098/1‑143 (MQ=255)                                                                 ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                                                                 <  2:1021140/143‑1 (MQ=255)                                                                 ggATTGAACGGCGAAGCGTGCGTGATCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                                                                 <  2:851166/143‑1 (MQ=255)                                                                   aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                               >  1:717287/1‑143 (MQ=255)                                                                     tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                             <  1:768461/143‑1 (MQ=255)                                                                      gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt                                                            <  2:764200/143‑1 (MQ=255)                                                                       aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                           >  1:929195/1‑143 (MQ=255)                                                                            cgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAGCTCTTTAGCGa                                                      >  1:615556/1‑143 (MQ=255)                                                                               aGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACt                                                   >  1:816316/1‑143 (MQ=255)                                                                                    gcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt                                              <  2:733522/143‑1 (MQ=255)                                                                                        gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTTCTCCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTTGCGAACTGCCTTCgaa                                          >  2:461875/1‑143 (MQ=255)                                                                                        gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa                                          >  1:229669/1‑143 (MQ=255)                                                                                        gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTCCCTTCgaa                                          >  1:276136/1‑143 (MQ=255)                                                                                         cactgCAGCTGGCTGAACGTAAAAAATGGGCGGTATTCTCTAAAGAGCCCATCCCGCCAGACGACGAGCGTGTGCTGCTTGGTTTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                         <  2:795812/139‑1 (MQ=255)                                                                                           cctgtgCTGATTGAACTTAACAAATCTGCGCTATTCTCTTAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCCAGCACCGCGATGGGTAAGCTCCCAACCGGAGTGTCTTCgaaaga                                       <  2:828599/138‑1 (MQ=255)                                                                                               aggtggctgcactgaaAAAATATGCGGTATTCTCTAAAGAGACCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaaa                                   <  2:1052556/137‑1 (MQ=255)                                                                                                 ctgacggaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACa                                 <  2:883358/143‑1 (MQ=255)                                                                                                  tgaccgaACTGAAAAAATATGCGGTATTCGCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg                                <  2:425709/143‑1 (MQ=255)                                                                                                   gactgcACTGTAGTAATATCCGGTATTCTCTAAATAGATCATCGCGCCAGACTACGATCGTGTGCTGCTTAGTGTTGCTGGTTTTCAGGCGTGCGCCGCGCTGGCAAATCTCTTTAGCGAATTGCCTTCGAAAGAAAAACAgg                               <  2:921304/143‑1 (MQ=255)                                                                                                          ctgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGACCTGCCTTCGAAAGAAAAGCAGGTAGTCac                        >  1:934654/1‑142 (MQ=255)                                                                                                          ctgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaa                        <  1:1004838/143‑1 (MQ=255)                                                                                                           tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAGTCTCTTTAGCGAACTGCCCTCGGAAGAAAAACAGCTAGTCaaa                       >  2:780297/1‑143 (MQ=255)                                                                                                           tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCCCGCCGCGCTGGCAAATCTCTTTCGCGAACTGCCCTCGCAAGACAAACCGGTAGTCaaa                       >  2:1062986/1‑143 (MQ=255)                                                                                                            gaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaag                      >  1:854147/1‑143 (MQ=255)                                                                                                             taaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaaga                     >  2:472327/2‑143 (MQ=255)                                                                                                             aaaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaaga                     >  2:889991/1‑143 (MQ=255)                                                                                                               caaaTCTGCGGGATTCGCTAAAGAGACCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag                   <  1:419908/142‑1 (MQ=255)                                                                                                                aaaTATGCCGTATGCTCTAAAGAGACCATCGCGCCAGACGACGAGGGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAgg                  <  2:397406/143‑1 (MQ=255)                                                                                                                     tGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa             <  1:888289/143‑1 (MQ=255)                                                                                                                     tGCGGTATTCTCGAAAGAGACCATCTCGCCAGTCGGCGAGCGCGCGCTGCTTGGTGCTCCCGGTTTTCAGGCGCGCGCCCCGCTGGCAAATCTCTTTAGCGAACTGCCTTCCAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa             <  2:242163/143‑1 (MQ=255)                                                                                                                        ggTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAACAAGGCGCGAccc          >  2:842267/1‑142 (MQ=255)                                                                                                                        ggTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGCTTTCAGGCGCGCCCCGCGCTGGCAAATCCCTTTAGCGAACTGCCTCCGAAAGAAAAACAGGAAGCCGACGAAGGcgcgcgca          >  2:894812/1‑139 (MQ=255)                                                                                                                             tctcTAAAGAGACCCTCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg     <  1:661317/143‑1 (MQ=255)                                                                                                                             tctcTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAACATCATCTAGTCAAAGAAGGCGCGACCACTTTg     <  2:828680/143‑1 (MQ=255)                                                                                                                               tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGCAGGCGCGACCGCTTTGCt   >  1:153249/1‑143 (MQ=255)                                                                                                                               tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCt   >  2:571356/1‑143 (MQ=255)                                                                                                                                ctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa  <  1:871830/143‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTA  >  NC_000913/3041499‑3041768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑