| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 695,766 | G→A | W222* (TGG→TGA) | ubiF → | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 695,766 | 0 | G | A | 98.0% | 145.7 / NA | 51 | W222* (TGG→TGA) | ubiF | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (21/28); minor base C (0/1); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCTGGTGCGTTTC > NC_000913/695626‑695905 | ggAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGc < 2:287645/143‑1 (MQ=255) gAATTGGCGTTCATGCATAGCAGTATCGGCAGTTTTGCATGTTGCTTAGAGTACAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTGGTTGGATGACTGAGCa > 1:438307/1‑143 (MQ=255) ttGGCGTTTGTGCCTGCCAGTTGGCGCCGGCGTGCATGTTGACTAGCGTCCAGTGCGAGAACGCTCCCGGCGACAGCACTTGGCAGCAATTTTCTTCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGATCATCg < 2:474078/143‑1 (MQ=255) ttGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCg > 1:564834/1‑143 (MQ=255) catgcgtgGCAGTATGCGCAGTCGTGCCTGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGtgtg < 2:442695/143‑1 (MQ=255) catgcatgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGtgtg < 2:48498/143‑1 (MQ=255) tgGCGGTCTGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGa < 2:555172/143‑1 (MQ=255) tgGCGGTAGGCGCAGTCGTTCATGTTGATTAGCTTCCAGTACGAGATCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGa < 2:555002/143‑1 (MQ=255) tgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGa < 1:1155745/143‑1 (MQ=255) tgGCAGCATGCGCCGTCGTGCATGTTGGTTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCTCCTTGCAGCAATTTACGCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGa < 2:1105122/143‑1 (MQ=255) gaTGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGTGCATCGCTGGTGTGGTATGACTCTcc < 2:309889/142‑1 (MQ=255) gcgcAGTCGGGCATGTTGGTTCTCGTCCAGTGCGAGGACGACCCCGGCGACAGCACCGGGCGGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGgc < 2:97796/143‑1 (MQ=255) gcAGGCGTGCATGGTGATGAGCGTCCAGGGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGgcgc < 1:236797/143‑1 (MQ=255) actCGTGCATGTTGACCAGCGTCCACCGCGAGAACGCTCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGt < 1:235470/141‑1 (MQ=255) gCATGTTGATTCGCGGCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGc < 2:322609/143‑1 (MQ=255) aTGTTGCTGAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGCTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCa < 1:1040155/143‑1 (MQ=255) ttGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGtt > 1:443091/1‑143 (MQ=255) aGCGTCTAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaa < 1:777457/143‑1 (MQ=255) aGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaa > 1:622450/1‑143 (MQ=255) cgtgccgTGCGAGAGCGATCGCGGCGACGGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCGGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaata < 2:642786/137‑1 (MQ=255) cGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaata < 1:40453/143‑1 (MQ=255) gTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatat < 2:464604/143‑1 (MQ=255) tGCGAGATGCATCCGGGCGACAGCAGGTGGCAGCAATTTACGCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaatat < 1:572977/143‑1 (MQ=255) tGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGTACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaatat < 1:679261/143‑1 (MQ=255) aaCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGAACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGCATTCGCCAGTTGCAGAATATGAATATGGcaca > 1:189057/1‑143 (MQ=255) tCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATGTGAATATGGCACAGCTcc > 1:274678/1‑143 (MQ=255) ccGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAg > 1:515298/1‑143 (MQ=255) cTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGa > 1:762214/1‑143 (MQ=255) cTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGa < 2:443091/143‑1 (MQ=255) gcaATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGCAGCAtg > 1:601980/1‑142 (MQ=255) gcaATTGTCTCCGGACGGACCGCGTGCGTGTCTGCCGTTGTTTGATAACGGAGCATCGCTGGTGTGGTAGGACTCTCCGGCGCGGATTCGCCCGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCAtt < 2:285349/143‑1 (MQ=255) aaTTTACTCCGGACGGACCGCGTGCATTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGCGTATGGCACAGCTCCAGGCGGAGATGGCGAGGCATTTc > 1:71959/1‑143 (MQ=255) aaTTTACGCCTGGCGGACCGCGGGCGTGTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCTCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTc < 2:711069/143‑1 (MQ=255) tACTCCGGACGGACCGCGGGCGTTTCTGCCGGTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGt < 1:455295/143‑1 (MQ=255) tCCGGACGTACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGTGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTcgc < 1:23665/143‑1 (MQ=255) tCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCAGGTGTGGTATGACTCAACGGCGCGTATTAGCCAGTTGCAGAATATGAATATGGCAACGCTCCAGGCGGAAAGCGCGGAGCAGTTCACGTcgg > 1:285956/1‑142 (MQ=255) tCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGAGAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTcgc > 2:348448/1‑143 (MQ=255) cggacggaCCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGt > 1:966308/1‑143 (MQ=255) cggacCGCGTGCGTGTCTGCCGGTGCATGATATATCAGCACCGGGGTTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTgg < 1:482718/143‑1 (MQ=255) cgcacCGCGTCCGTTCCTGCCGTTGTTTTAAAACTGCGCATCGTTGGTGTGGTATGACTCTCCGGCGCATATTCGCCAGTTGAAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCTAAGCATTTCCCGTCGCGTCTgg < 1:885021/140‑1 (MQ=255) cgtgcgtTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGGCGCGTCGGGggtacgt > 2:365921/1‑143 (MQ=255) tgcgtTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGgttacgtta > 1:521253/1‑143 (MQ=255) ggcgtTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGgttacgtta < 1:705988/142‑1 (MQ=255) tttCTGCCTTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACAcc < 1:551857/143‑1 (MQ=255) tttCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACAcc > 2:1068750/1‑143 (MQ=255) cTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCt > 1:919010/1‑143 (MQ=255) tGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGAGTCTGGGTTAGGTTACACCGGtt > 2:717822/1‑143 (MQ=255) ttgttTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTCGGTTACGTTACACCGCTTGCCGc > 1:1006421/1‑143 (MQ=255) tgttTGATAACTGAGCATCGCTGGTGTGGTATGCCTCTCCGCCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCt < 1:922066/143‑1 (MQ=255) gATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCAGTTCCCGTCGCGTCTGGGTTACGCGACACCGCTCGCCGCTGGTGc > 2:673121/1‑143 (MQ=255) cTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCTGGTGCGTTTc > 1:317945/1‑143 (MQ=255) | GGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCTGGTGCGTTTC > NC_000913/695626‑695905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |