| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,123,110 | G→A | P77L (CCA→CTA) | ftsN ← | cell division protein FtsN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,123,110 | 0 | G | A | 94.2% | 144.8 / NA | 54 | P77L (CCA→CTA) | ftsN | cell division protein FtsN |
| Reads supporting (aligned to +/- strand): ref base G (0/2); major base A (25/24); minor base C (1/2); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGC > NC_000913/4122971‑4123249 | ttCCAGATGCTGGCGTTGCTGTGGTGTCAGTTGCTCCGGCGTTTGCACTTCACCACCGGCAGACGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTGCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtg < 2:490497/143‑1 (MQ=255)ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtg < 1:19278/143‑1 (MQ=255)ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtg < 1:324655/143‑1 (MQ=255) gcgagaagCTGACGTTGTTCTCGTGTCAGTTGCGCCGGCTTTTTCACTTCACCACCGGCAGAAGGTTCTGTGTGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtgg < 2:849116/140‑1 (MQ=255) gacgCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTGTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGt < 2:315322/140‑1 (MQ=255) aagCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTc > 2:334999/1‑143 (MQ=255) cTGACGTTTTTCTGCTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGt < 2:813285/143‑1 (MQ=255) gttgttCTGGTGTCAGTTTCTCCGGCGTTTTCACTTCACTACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTGCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg < 1:182918/143‑1 (MQ=255) gttgttCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg < 2:984543/143‑1 (MQ=255) gttgtgCTGGTGGCAGTTGCGCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg < 1:1061271/143‑1 (MQ=255) gttCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGTCTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTc < 2:312720/143‑1 (MQ=255) gtgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCCGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCGCtttg > 1:744/1‑142 (MQ=255) gtgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTc > 1:184951/1‑143 (MQ=255) gtgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGGTTTAGTGGTAGTCCGTTTCCGGTCCCTGTc > 2:1080755/1‑143 (MQ=255) tgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCGGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTGTTAGTGGTAGTCCGTTTCCGGTGACTTTCt > 1:10191/1‑143 (MQ=255) tCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACATTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTgg < 2:921212/143‑1 (MQ=255) aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACGTTTCAGCTTTTTAATGTAGCGCCAGCGTTCTCCGGGTCGTAGTGGTACTCCGTGTCCGGCCACTTTCTGGCt > 2:461993/1‑143 (MQ=255) ccccagCGTTTTCGCTTCACCCCCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCa < 1:1024266/138‑1 (MQ=255) gCGTTTTCACTTCACCACCGGCAGAAGGTTCGGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCCGCGTTCTTCTGGGTTTAGTGGTCGTCCGTTTCCGGTCACTTGCTGGCTTTGCATCgac > 2:48039/1‑141 (MQ=255) ttttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCCCTTTCTTGCTTTGCAGCGtctcc > 2:546200/1‑142 (MQ=255) ttttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCg > 1:1138219/1‑143 (MQ=255) acttcacCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGACTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTGCCGGActc > 1:509652/1‑143 (MQ=255) ttcacCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACtctt > 1:76544/1‑143 (MQ=255) ccaccGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCttcttt < 1:815674/143‑1 (MQ=255) ccaccGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCttcttt > 1:922425/1‑143 (MQ=255) accGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCttctttct < 1:804223/143‑1 (MQ=255) ccGGCAGAAGGTTCTTTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCGCTTTAATGTAGCGCCAGCGGTCGTCTGGTCCTCGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTGTCGGACTCttctttcgt > 1:631524/1‑141 (MQ=255) ccGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTtctttctt > 1:31685/1‑143 (MQ=255) cGGCAGTAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTGATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtg < 2:930855/143‑1 (MQ=255) cagctgtttCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGCAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGCCTCTTCTTTCTTGTGa < 2:781367/136‑1 (MQ=255) cAGAAGGTTCTGTGGGCGCACGCACTCCTGGCTCGCTCCTTTCCAGCTCTTTAATGTAGCTCCATCGTTCTTCTGGTTTTAGTGGTAGTCCGCTTCCGGCCACGGTCTGGCTTGGCAGCGTCTCGGACTCTTCTTTCTTGTGa < 1:169627/143‑1 (MQ=255) gaGGTTCTGTGGGCGCACGCAGCGCCTGCTGGCGACTCTCCAGCTCTTTGATGTGCCGCCAGTGTTCTTCTGGTATTAGTGGTAGTCCGTTTCCGGTAACTGTCTGGCTTTGCGGCGTCTCGGAATCTTCTTTCTTGTGATGc < 2:31685/142‑1 (MQ=255) aaGGTTCTGTGGGCGCACGCTCTGCCTGCTGGCGACTTTCCAGCTCTTTTATGTAGCGCCAGCGTTCTTCTGGTTTTCTTTGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGTAGCGTCTCGGATTCTTCTTTCTTGTGATGc < 2:1025733/143‑1 (MQ=255) aaGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGc > 1:338460/1‑143 (MQ=255) ggTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTCTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGCACTCTTCTTTTTTGTCATCCGt > 2:434453/1‑143 (MQ=255) gtgGGCGCACGCTCTCCCGGCTGGCGTCTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTGTGGTTTTAGGGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGaa < 2:97230/143‑1 (MQ=255) ggggCGCGCGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAg < 1:71595/142‑1 (MQ=255) gcacgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAg < 1:523369/143‑1 (MQ=255) cacgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGa < 1:1080755/143‑1 (MQ=255) cacgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGa < 2:1138219/143‑1 (MQ=255) acgcgcTCCCGGCTGGCTACGTTCCAGCTCTTTAGTGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGac < 2:140683/143‑1 (MQ=255) gcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTGGTGATGCGTAATGAAGTACAGacca > 1:328118/1‑143 (MQ=255) cacTCCCGGCTGGGGACTTTCCCGCGCTTCGCTGGAGCTCCGGCGTGCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGaccac < 2:10191/143‑1 (MQ=255) acTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCCGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTACTGACGTACAGaccacc > 2:497546/1‑143 (MQ=255) tCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTCGTCCGTTTCCGGTCACTTTCTGGCTTTGCGGCGTCTCGGACTCTTCTTTCTTGTGCTGCGTACTGAAGTACATACCACCgt > 1:79689/1‑142 (MQ=255) gCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAg < 1:546200/143‑1 (MQ=255) gCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAg < 1:334999/143‑1 (MQ=255) aGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc > 1:698757/1‑143 (MQ=255) tGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATa > 1:182069/1‑143 (MQ=255) ttgcgcCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGCACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGGCCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGc < 2:76544/141‑1 (MQ=255) tAGCGCCAGCGTTCCTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACCTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGc < 1:1066317/143‑1 (MQ=255) tcgcAGCGTTGTTCTGGTTTTGGGGGTGGTGAGTTGCCTGACTCTTGCGGGCTTTGCAGCGTCTCGGGCTCTTCTTTCTTGTGATGCGTAATGACGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGAc < 2:1121253/140‑1 (MQ=255) cAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCAt > 1:659725/1‑143 (MQ=255) gTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCg > 1:918032/1‑143 (MQ=255) tcttctGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAACGTCACAAGACCGGCGGCAGCAATAGCGACCATAGCggg > 1:696448/1‑143 (MQ=255) tGGGTTTCGTGGTAGTCCGTTTCCGGTCTCTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGGAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGaaa < 2:841342/143‑1 (MQ=255) tttAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCgc > 1:813852/1‑143 (MQ=255) tttAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCgc > 1:548767/1‑143 (MQ=255) | TTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGC > NC_000913/4122971‑4123249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |