| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 92.0% | 115.1 / ‑5.3 | 51 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base T (15/31); total (19/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388 | ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 1:981290/143‑1 (MQ=255) agaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGCGACGAGCATATGCACATCACCAGCACCGAAGAAAAACGATGAATc > 2:806764/1‑143 (MQ=255) gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAACCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATcc < 2:47942/143‑1 (MQ=255) cgtcgaCGGCGGTTCGTTTGCCGGCGGCAGAGAAACCATGGAACTCGGCGAGCTGCTGGGAGCCCTCAATCATGCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCTGCACCGAAGAAAAACTCTGGATCCAAc < 2:1050058/138‑1 (MQ=255) cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGGATATGCACATTACCAGGACCGAAGAAAAACGCTGGATCCAAc > 2:736066/1‑143 (MQ=255) ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCCTGCCCCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 1:11219/143‑1 (MQ=255) gtCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 1:506766/142‑1 (MQ=255) ggagggcGGTTGATTTGCGAGCGGCAAAGAGACGATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAGCCTCCTGCGGCCCGATTGGTGCCGTGTATCTGCGTCGTTCCCGCGCCGAAGAAAAACTCTGGATCCAACAGc < 2:356707/137‑1 (MQ=255) caccgccGGTGCATTTGCCAGCGGCACAGAAAACATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGc < 2:41834/137‑1 (MQ=255) aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGt < 2:847775/143‑1 (MQ=255) gTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTAAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACCTTACCAGCACCGCAGGACAACTCTGGATCCAACAGCGtct > 1:542472/1‑141 (MQ=255) cGGTTCATTTGCCAGCGGCGAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCg < 1:596335/143‑1 (MQ=255) gTTCATTCGCCAGCGGCAAAGAAACCATTAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg < 2:616446/143‑1 (MQ=255) ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCGCATTACCAGCCCCGAAGCAAAGCTCTGCATCCAACGACGTATCgcgc > 2:39676/1‑140 (MQ=255) tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCttg > 1:509210/1‑141 (MQ=255) ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATAGGTGCCGCGAATATGCACATTACCAGCACCGAAGAAAGACGATGCATCCAACAGCGTATAGAGTCTGGTcac > 2:1054160/1‑141 (MQ=255) ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACATACTGCGGCCAGATTGGTGCCGAGTCTACGCACATTACCAGCACCGAAGAAAAACTCAAGATCCAACAGCGTATCGAGTCTGGTcgc > 2:505795/1‑143 (MQ=255) ggCAAAGAAACCATGAAACGCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGAc < 1:205100/143‑1 (MQ=255) gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAGATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcgcgccg > 1:799460/1‑140 (MQ=255) gccGGCGTGCTGCTGGCCGCACTGTAGCAAACCTACCGCGGCCCGATNGGTGCTGAGTACAGCCACATCACCAGCACCGAAGACAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaa < 2:73001/141‑1 (MQ=255) tCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaa > 1:895300/1‑143 (MQ=255) gCGAGCGGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTACCGAGCCTGATCACGCGACTCTCAATAGCGACGAGaaaaaa > 2:471748/1‑143 (MQ=255) ccgttgtaaGTCCGCAATCAAACCTTCTGCGGCCCGATTGGTGTCGAGTATATGTACATTATCAGCACTGAGGTAAACCTCTGTATCAAATAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGctt < 2:33849/136‑1 (MQ=255) gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCTATTGGTGCCGAGTATATGCATATAACCAGCAAAGCCAATGTACTCTGGATCCAACAGCGTATCGACTCTGGTCGCGCGCCTTTCCATAGCGAAGAGCAAAACCGcttct > 2:822647/1‑143 (MQ=255) gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCCGCACCGAAGAAAAACTCTGGATCCAACAGCGCATCGAGTCTGGCCGCGCCACTCTCAATACCGAAGCGAAAAAACGcttct > 2:534095/1‑143 (MQ=255) cgggAAGCCCGCAACCAAACCTACTGCGGCTGGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt < 1:374602/141‑1 (MQ=255) ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa < 1:1034866/143‑1 (MQ=255) ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa < 1:1078667/143‑1 (MQ=255) gAAGCCCTCAAACAACCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg < 1:1086352/143‑1 (MQ=255) aaGCCCTCAAGCAAACCTCCCGCCGCCCGAGTGGTGCCGAGTATCTGGGTGTGACCACCACCAAAGATAAGCTCGTTATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGc < 2:1126839/143‑1 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt < 1:872298/143‑1 (MQ=255) tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACCCTGGACCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCAAAcct > 2:495469/1‑141 (MQ=255) aaGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATAGGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 2:662608/143‑1 (MQ=255) gCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGt < 1:948684/143‑1 (MQ=255) cGGCGCTATCGGTGCCGAATATACGCACAGTACCAGCACCGAAGAAAAACTCTTGATCCAACAGCGTATCGAGTCTGGTCGCGCCACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTc < 1:912399/143‑1 (MQ=255) ggCCCGATTGGTGCCGAGGATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCt < 1:1136210/143‑1 (MQ=255) acccGATTGCCGCCGAGTATACGCACATTCCCAGCACCGAATAAAAACTCTGGATCCAACAGCGCATCCAGTCTCGACGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 1:163657/142‑1 (MQ=255) cccGATTGGGGACGAGTAACTGCACAGTACCGCCACCGGAGAAAAACTCTGGAGCCAACAGCGTATCGAGTCTGGTCGCGCGACTATCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg < 2:1142804/143‑1 (MQ=255) caTTGGTTCCGAGTATATGCACATTCCCAGCACCGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc < 2:596377/142‑1 (MQ=255) tGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGtt > 1:454367/1‑143 (MQ=255) gTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAc < 1:1054160/143‑1 (MQ=255) ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTc < 1:622469/143‑1 (MQ=255) gcgttgtatGCACAGTACCAGCGTCGGGGGAACACTCCGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAAGAGCGATGAGAAAAAACGCTTCTTAAGCGAACTGGCCGCCGCTGAAGGTCTTGAACGTTACCTCg < 2:185693/137‑1 (MQ=255) gAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCgg > 2:43866/1‑143 (MQ=255) agcatatGCACACTACCCGCCCCGAAGAAAAGCTCTGGACCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAAGAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc < 1:55130/140‑1 (MQ=255) atatGCGCAGTACCGGCCGCGCAGAAAGACTCTGGAGCCAACAGCTTATCGAGTCTGGTCGCGCGACTTTCAATGGCGATGAGAAAACACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCa < 2:463434/143‑1 (MQ=255) acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGACGCAAAATTc > 2:487347/1‑143 (MQ=255) cccgCACCGACGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAACAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc < 1:54827/140‑1 (MQ=255) cAGCACCGAAGAAAGACTCTGGATCGAAAAGCGTATCGAGTCTGGTCTCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg < 2:1063798/143‑1 (MQ=255) cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaa > 2:403577/1‑143 (MQ=255) aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAACATTCCCTGGCGCAAAACGc > 2:54608/1‑143 (MQ=255) aaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTAGCGAAGAGAAAAACCGCTTCTTCAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTtccc > 2:695332/1‑141 (MQ=255) | CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |