Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 962,670 A→G K226E (AAA→GAA)  rpsA → 30S ribosomal subunit protein S1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913962,6700AG98.2% 167.4 / NA 55K226E (AAA→GAA) rpsA30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (29/24);  minor base T (1/0);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCAGAGCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCGC  >  NC_000913/962530‑962806
                                                                                                                                            |                                                                                                                                        
gcgcAGAGCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGgaa                                                                                                                                        >  2:16074/1‑143 (MQ=255)
            aTCAGCCGCCGGAACACCTGCCGGAAGGCATGGCAGTTAAAGGTATCGTTAATAACCTCACTGACTACGGTGCATTCGTTGATCCTGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCAt                                                                                                                            <  1:453305/143‑1 (MQ=255)
                gctgctGGGAATGCTGCAGGAAGGCATGGATGGTGAAGGGATGGTGAGGAACCTCACTGACTACGGTGCAGTCGTTGATCTGGGCGGCGTTGACGTCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGa                                                                                                                        <  2:222029/143‑1 (MQ=255)
                  tggtgGAAAACCTGCGGGAAGGCATTGAAGTTAAACGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGc                                                                                                                      <  1:197378/140‑1 (MQ=255)
                  tgctgGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGc                                                                                                                      <  2:154037/143‑1 (MQ=255)
                   gctgGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCg                                                                                                                     >  1:287135/1‑143 (MQ=255)
                     tgGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGACCGaa                                                                                                                   >  2:872490/1‑143 (MQ=255)
                             cTGCAGGAAGGCATGGAAGTTACAAATATGTGGAAGAACCTCACTTTCTTGTGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCaa                                                                                                           <  1:1031707/143‑1 (MQ=255)
                             cTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCaa                                                                                                           >  1:609185/1‑143 (MQ=255)
                             cTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCaa                                                                                                           <  1:925307/143‑1 (MQ=255)
                                   caaGGCACGGAAGTCACACGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTggg                                                                                                     <  1:966468/142‑1 (MQ=255)
                                       gCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATCACCTCGTTCAAAACTAAGGATCCGAGCGAAATCGTCAACGTGGGagac                                                                                                 >  2:239524/1‑143 (MQ=255)
                                        cagggAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGcgacg                                                                                                <  1:554840/140‑1 (MQ=255)
                                         aTGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGACTGCTGCCCATCACTGACATGGCCTGGGAACGCGTTAGGCATCCGAGCGAAATCGTCAACGTGGGcgacga                                                                                               >  1:216758/1‑143 (MQ=255)
                                               gTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCAc                                                                                         <  1:471644/143‑1 (MQ=255)
                                                ttAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAACGAAATCGTCAACGTGGGCGACGAAATCACt                                                                                        >  2:143170/1‑143 (MQ=255)
                                                ttAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTAGTTGGTCTGGGCGGCGTTGACGGCCTGCTGCACGTCACTGACATGGCCTGGGGACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGGAATcgat                                                                                        >  2:147265/1‑140 (MQ=255)
                                                  aaaGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGt                                                                                      >  1:672986/1‑143 (MQ=255)
                                                     ggTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTaa                                                                                   >  1:295637/1‑143 (MQ=255)
                                                      gTATCGTTAAGAACCTCACTGACTACGGAGCATTCGTTTATCTGTGAGGCATTGACGGCCTGCTGCACATCACTGACTTGGCCTGGCAACGAGTTAATCGTCCGAGCGAAGTCGTCAACGTGGGCGGCGAACTCACAGttgac                                                                                  >  2:1010073/1‑140 (MQ=255)
                                                          cGTTAAGACGCTCACTGAGTACGGTGCATTCGTTGCTCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGc                                                                              <  1:1012158/143‑1 (MQ=255)
                                                                 aaCCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGATAAAATGCTGAAGta                                                                       >  1:973239/1‑142 (MQ=255)
                                                                  aCCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTc                                                                      <  1:1050030/143‑1 (MQ=255)
                                                                  aCCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCAGCACATCACTGACATGGCCTGGGAACGCGTTAAGCGTCCGAGCGAAATCGTCAACGTAGCCGACGCAATCACAGGTAGAGTGGTGAAgagc                                                                      >  1:127226/1‑140 (MQ=255)
                                                                   ccTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTGAGCATCGGAGCGAAGTCGTCAACGTGGGCGACGAAATAACTGTTAAAGTGCTGAAGGTCg                                                                     >  1:351612/1‑143 (MQ=255)
                                                                    cTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGa                                                                    >  1:1021408/1‑143 (MQ=255)
                                                                    cTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGa                                                                    <  1:165454/143‑1 (MQ=255)
                                                                     tCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACCGTTAAAGTGCTGAAGTTCGAc                                                                   >  1:356110/1‑143 (MQ=255)
                                                                       aCTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCAGAGCGAAACCGTCAACGGGAGCGACGAAAGCACTAGTACAGTCCTGAAGCTCCACcg                                                                 >  2:862753/1‑143 (MQ=255)
                                                                            cTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAAc                                                            <  1:16074/143‑1 (MQ=255)
                                                                             tACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACg                                                           >  1:498749/1‑143 (MQ=255)
                                                                                     aTTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGCGACCGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCgtg                                                   <  1:42102/143‑1 (MQ=255)
                                                                                            gATCTGGGCGGCCTTGACGGCCTGCTTCACATCTCCGACATGGCCTGCGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGTGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCt                                            <  1:847870/143‑1 (MQ=255)
                                                                                              tCTGGGGGGCGTTGACGGCGTGGTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTgg                                          <  1:399227/143‑1 (MQ=255)
                                                                                                  ggcggcGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGGACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGACATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTAACCGTGTACCCAGGGGCCt                                      >  2:965795/1‑143 (MQ=255)
                                                                                                  ggcggcGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCt                                      >  2:486874/1‑143 (MQ=255)
                                                                                                   gcggcgTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTg                                     >  1:279213/1‑143 (MQ=255)
                                                                                                    cggcgTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGa                                    <  2:287135/143‑1 (MQ=255)
                                                                                                      gcgTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGaaa                                  <  2:242585/143‑1 (MQ=255)
                                                                                                                  tgctgcACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCATGGGCCTGGAACAGCTGGGCGaa                      >  2:1057569/1‑143 (MQ=255)
                                                                                                                   gctgcACATCCCTGAACTGGCCTGGGAACGCGTTAAGCATCCGAGTGAAATAGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAg                     <  1:842150/143‑1 (MQ=255)
                                                                                                                    ctgcACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCGGAGCGAAGa                    >  2:64681/1‑143 (MQ=255)
                                                                                                                     tgcACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGAt                   <  2:672986/143‑1 (MQ=255)
                                                                                                                         gaTCAGTGACTTGGCCTGGGAACGCGTTAAGCATCCGGGCGAAGTCGTCAGCGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTAACCGTGTATCCCTGGGCCTGAAACAGCTGGGCGACGATCCGt               <  2:1080489/142‑1 (MQ=255)
                                                                                                                          aTCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTg              >  1:378633/1‑143 (MQ=255)
                                                                                                                          aTCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTg              >  2:227696/1‑143 (MQ=255)
                                                                                                                          aTCACTGACATGGCCTGGGAACGCGTGAAGCATCCGAGCGAAATCGTCAACGTGGACGACGAAATCACTGTTAACGTGCTGAAGTTCGACCGCGAACGTACCCTTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTg              >  2:1105187/1‑143 (MQ=255)
                                                                                                                           tCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGCACCAGCTGGGcgacgacaccgtt             >  1:836408/1‑136 (MQ=255)
                                                                                                                           tCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTgg             <  1:1123268/143‑1 (MQ=255)
                                                                                                                                 aCATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCt       >  2:807854/1‑143 (MQ=255)
                                                                                                                                   aTGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTAt     <  1:664340/143‑1 (MQ=255)
                                                                                                                                     gggcTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAGCGATAGATGGATTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCg   <  1:534703/140‑1 (MQ=255)
                                                                                                                                     ggCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGCTCCGTGGGTCGCTACCg   >  2:725505/1‑143 (MQ=255)
                                                                                                                                     ggCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGACGACGATCCGTGGGTCGCGATCg   >  2:585556/1‑143 (MQ=255)
                                                                                                                                      gCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGGAGATCCGTGGGTAGCTGTCGc  >  2:366914/1‑143 (MQ=255)
                                                                                                                                      gCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGCTCGGTGGGTAGCTATCGc  >  1:693021/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                        
GCGCAGAGCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCGC  >  NC_000913/962530‑962806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑