Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,019,309	C→A	D249Y (GAT→TAT)	ompA ←	outer membrane porin A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,019,309	0	C	A	95.5%	188.4 / NA	67	D249Y (GAT→TAT)	ompA	outer membrane porin A
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (33/31);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATCCAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCGGTGCCGGAGCTGGAGC  >  NC_000913/1019170‑1019447                                                                                                                                            |                                                                                                                                           ctCTTGTCCGCCGGGAGACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGTTGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAg                                                                                                                                         <  1:851456/142‑1 (MQ=255) aTCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAg                                                                                                                                         >  1:203085/1‑143 (MQ=255) aTCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAg                                                                                                                                         >  2:627254/1‑143 (MQ=255)   cTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGCACAACTACGGAACCGTCTTTCGGATCCAGGt                                                                                                                                       >  1:610899/1‑143 (MQ=255)   cTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGACCCGTCTTTCGGACACAGGt                                                                                                                                       >  2:987133/1‑143 (MQ=255)      gTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGc                                                                                                                                    >  1:30277/1‑143 (MQ=255)      gTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCACCACAACTACGGCACCGCCTTTCGGCTCCACGTTGc                                                                                                                                    >  1:65159/1‑143 (MQ=255)           ccGGGAGACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAgc                                                                                                                               <  2:384339/143‑1 (MQ=255)                    cTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCGCGGACAGACCCTGGTTGTAAGCGTCAGAACCGCTGCGGTCGGCGTAACCCAGAACAACTACGCAACCGCCTCTCGGATACAGCTTGCTCCCCTGCCCGTAc                                                                                                                      >  2:3717/1‑143 (MQ=255)                     tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGCTACAGGTTGCTCAGCTGGCTGTAcc                                                                                                                     >  2:286991/1‑142 (MQ=255)                     tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTAGGTGTGACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACa                                                                                                                     >  2:404173/1‑143 (MQ=255)                      ttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAg                                                                                                                    <  1:230333/143‑1 (MQ=255)                          agaTCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGGCCCGGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGa                                                                                                                <  2:984918/143‑1 (MQ=255)                          agaTCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTGCAGCTGa                                                                                                                >  1:106679/1‑143 (MQ=255)                            gtCAGGTGAGCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAGCCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGGCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATc                                                                                                              <  2:765735/142‑1 (MQ=255)                            aTCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACGGCTgctg                                                                                                              >  2:902795/1‑140 (MQ=255)                                 gtaacgaacaacAGACGGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCGagag                                                                                                         <  2:793546/137‑1 (MQ=255)                                 gTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGATCGGTGTAACCCAGAACAACTACGGAACCGTCTGTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCagag                                                                                                         >  2:74823/1‑143 (MQ=255)                                  tAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGTATGCTCAGCTGGCTGTACAGCTGATCCAGagc                                                                                                        >  1:209963/1‑143 (MQ=255)                                      caacaaCAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTAAGCTGGCTGTACAGCTGATCCAGAGCAGcc                                                                                                    >  2:972597/1‑143 (MQ=255)                                       aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCt                                                                                                   >  1:867155/1‑143 (MQ=255)                                        ccaacaGACTGAGCACGGCGCTCGGACGGACCTTGGTTGTAAGCGTCAGATCCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTg                                                                                                  <  2:924553/142‑1 (MQ=255)                                          aacaGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGAc                                                                                                >  1:959094/1‑143 (MQ=255)                                           acaGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGAcc                                                                                               >  1:451080/1‑143 (MQ=255)                                                cTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCg                                                                                          <  2:211067/143‑1 (MQ=255)                                                  gAGCACGGGGCGCGGACAGACCCTGGTTGTAAGCGTCAGAGCAGCTAAGTTTAACTTAACCCAGGGCGTCTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGt                                                                                        <  1:790251/143‑1 (MQ=255)                                                    gCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTATGAACCGATGCGGTCGGCGTAACCCAGAACCACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGttt                                                                                      >  2:700847/1‑143 (MQ=255)                                                      aCGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCa                                                                                    <  1:340427/143‑1 (MQ=255)                                                            cTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTg                                                                              <  1:793068/143‑1 (MQ=255)                                                            cTCGGACAGACCCTGGCTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGCACAACTACGGAACCGTCTCTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTg                                                                              <  2:7097/143‑1 (MQ=255)                                                                  cAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTttgt                                                                        <  2:340333/143‑1 (MQ=255)                                                                   aGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTttgtt                                                                       <  1:288815/143‑1 (MQ=255)                                                                     aCCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTgttga                                                                     >  2:819499/1‑143 (MQ=255)                                                                     aCCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTgttga                                                                     >  1:880494/1‑143 (MQ=255)                                                                      gctcggTTGTACGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAGCTGCGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTgttgaa                                                                    <  2:559743/139‑1 (MQ=255)                                                                      ccccGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCGGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTgttgaa                                                                    <  1:853833/143‑1 (MQ=255)                                                                        cagggtGTATGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGGCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGCCCTTCCGGTTGCAGGGTGGCGTTgttgcagt                                                                  >  2:417525/6‑143 (MQ=255)                                                                          ggTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTgttgaagttg                                                                <  1:389053/143‑1 (MQ=255)                                                                            ttGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTgttgaagttgaa                                                              <  2:209557/143‑1 (MQ=255)                                                                            ttGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTgttgaagttgaa                                                              >  2:847602/1‑143 (MQ=255)                                                                              gTAAGCGGCAGACCCGATGCGGTTGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGTTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACa                                                            <  2:994815/143‑1 (MQ=255)                                                                                aaGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGa                                                          >  1:37269/1‑143 (MQ=255)                                                                                aaGCGGCAGATCCGATTCGGTCGGTGTAACGCAGAACAACTACGGAACCGTCTTTCAGATACAGGTTGCTAAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGa                                                          <  2:298955/143‑1 (MQ=255)                                                                                    gTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGAGACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGt                                                      <  2:203085/143‑1 (MQ=255)                                                                                     tCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGCACGTc                                                     >  1:170239/1‑143 (MQ=255)                                                                                     tCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGGTTCGGGGTTGCTTTGTTGAAGTTGAACAGAACGTc                                                     >  1:955428/1‑143 (MQ=255)                                                                                        gAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGa                                                  <  1:531468/143‑1 (MQ=255)                                                                                              aTGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTGCag                                            >  1:701306/1‑143 (MQ=255)                                                                                                 cggtcggtGTAACCCAGAACGACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCGGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGt                                         <  2:848402/143‑1 (MQ=255)                                                                                                     cggtGTAACCCAGAACAACTACGGACCCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAg                                     <  1:421341/143‑1 (MQ=255)                                                                                                        tgtAACCCAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGCAGTTGAGCAGAACGTCAGACTTCAGAGTGACGTGc                                  >  2:870938/1‑143 (MQ=255)                                                                                                           aaCCCACAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTg                               <  1:987133/143‑1 (MQ=255)                                                                                                             cccAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGt                             >  1:8534/1‑143 (MQ=255)                                                                                                              ccAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTc                            >  1:1127186/1‑143 (MQ=255)                                                                                                              ccAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTc                            >  1:546546/1‑143 (MQ=255)                                                                                                               cAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCGTTGTTGAAGTTGAACAGAGCGTCAGACTTCAGAGTGGAGTGCGTGGTCt                           >  1:170528/1‑143 (MQ=255)                                                                                                               cAGAACAACTACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGGTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGCGTGAAGTGCTTGGTCt                           >  2:956679/1‑143 (MQ=255)                                                                                                                       cTACGGAACCGTCTTTCGGATACAGGCTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTcc                   <  1:404173/143‑1 (MQ=255)                                                                                                                        tACGGAACCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCg                  >  2:603388/1‑143 (MQ=255)                                                                                                                              aCCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCCGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCGGTGCCg            <  1:109584/143‑1 (MQ=255)                                                                                                                              aCCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCGGTGCCg            <  2:486524/143‑1 (MQ=255)                                                                                                                              aCCGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCGGTGCCg            <  1:955021/143‑1 (MQ=255)                                                                                                                                cGTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCGGTGCCGGa          <  1:69526/143‑1 (MQ=255)                                                                                                                                 gTCTTTCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCGGTGCCGGAg         >  1:677752/1‑143 (MQ=255)                                                                                                                                    tttCGGGTCCAGGTTCCTCAGCTTGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGCCTTAAGACTGGTTTGCTAGATAAGTATTACCGGTGCCGGAGCTg      <  2:467231/143‑1 (MQ=255)                                                                                                                                      tCGGATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCGGTGCCGGAGCTGGa    <  1:627254/143‑1 (MQ=255)                                                                                                                                        ggATACAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCGGTGCCGGAGCTGGAGc  >  1:1081595/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                           ATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGAACCGTCTTTCGGATCCAGGTTGCTCAGCTGGCTGTACAGCTGATCCAGAGCAGCCTGACCTTCCGGTTTCAGGGTTGCTTTGTTGAAGTTGAACAGAACGTCAGACTTCAGAGTGAAGTGCTTGGTCTGTACTTCCGGTGCCGGAGCTGGAGC  >  NC_000913/1019170‑1019447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑