| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,868,859 | Δ1 bp | 11.4% | intergenic (+17/‑96) | yeaG → / → yeaH | protein kinase YeaG/DUF444 domain‑containing protein YeaH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,868,859 | 0 | C | . | 11.4% | 108.1 / 11.3 | 44 | intergenic (+17/‑96) | yeaG/yeaH | protein kinase YeaG/DUF444 domain‑containing protein YeaH |
| Reads supporting (aligned to +/- strand): ref base C (21/18); new base . (3/2); total (24/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
GTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAA > NC_000913/1868722‑1868997 | gTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTAAACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGAGCGTACGTAAATAGTCTTAACAAACATGGCCAGATACACAT‑g > 2:714513/1‑143 (MQ=255) acgaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCggg > 1:521020/1‑143 (MQ=255) acgaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACAT‑GTGTACCggg < 1:500277/143‑1 (MQ=255) aaggggggaagaaACACGACGACGTTGTCGCCCGTGTGACGGAAAAAGGCGACACCCGTCTCGGGGTGAACACACCAACCACCTGGCAGTCACGGAGACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCggg < 1:421918/136‑1 (MQ=255) gCAGAAGAAACACGACGAGTCGGTCGACCGTATGATGGAAAAAGGATACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGGG‑TACCGGGccag < 2:198532/143‑3 (MQ=255) gacacGACGGGTTTGTCGACCGTCTCGCACAAACACGCTACACCCGTAGACGGGTGCGTTTACTGTGCGAATGGTATTGGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACa < 2:183122/142‑1 (MQ=255) aaCACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACa < 1:598685/143‑1 (MQ=255) gacgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACCGCGaa > 2:281804/1‑143 (MQ=255) cTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGAAAGCGACCCgag > 2:93012/1‑141 (MQ=255) tCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGt < 1:514691/137‑1 (MQ=255) tCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGt > 2:514691/1‑137 (MQ=255) cGACCGTCTGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCGGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTg < 2:48717/143‑1 (MQ=255) tATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGc > 2:115142/1‑143 (MQ=255) tgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTCCGTAAATCGTCTTAACAACCCTGGCCCGGTACGCCT‑GT‑TACCGGGCCTACAGCGACAGCGAACCGTGGGCCGGACAAGCgc > 2:718876/1‑142 (MQ=255) atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAGGCGGCa > 2:820202/1‑143 (MQ=255) gggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCGTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa < 1:311569/142‑1 (MQ=255) aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaagcg > 1:167532/1‑140 (MQ=255) aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAacac < 2:413563/143‑1 (MQ=255) aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACGGTGGGCCTGAGAAGGGGCAAcaca > 1:106212/1‑143 (MQ=255) aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGc < 1:792551/143‑1 (MQ=255) gCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAAACGTGGGACTGAAAAGCGGCAACACA‑GGCGt > 1:508437/1‑143 (MQ=255) cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTa < 2:207720/143‑1 (MQ=255) acccccGTAATCTGGGGCTGTTGCTGTGCCTATGGTTGTTGCGCGTCCGTAAATCGTCTTAACAACCCTGGCCCGGTACCCAT‑GT‑TCCCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGc < 2:819291/140‑1 (MQ=255) ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACTGGGCCTCCAACGACCGCGATCCGTGGGCCTGAGAAGAGGCACAACA‑GGCGTAGCATAc > 2:613877/1‑143 (MQ=255) cGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCATACAACGACAGCGAACCGTGGGCCTGCGAAGCGGCAACAAA‑GGTGTAGCATAct > 2:830869/1‑142 (MQ=255) aaaCAGGTGCGTTGACTGTGCGAATGGTATTGGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGtt < 1:273051/143‑1 (MQ=255) aaCAGGTGGGTTTACGGTGCGAATGGTATTGGCGCGTACGTAAAGCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TGCCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTg < 2:455693/143‑1 (MQ=255) ttACTGTGCTAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAA‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATgtagt < 1:386992/143‑1 (MQ=255) gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACggggg < 1:713723/143‑1 (MQ=255) cGAATGGTAGTTGCGTCTACTTAGACCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGACCCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACgggggg < 2:572087/143‑1 (MQ=255) gcaTGGTAGGTACGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATGCAGTTGGCGAATGTAGTACGAGGGGc > 2:606791/3‑143 (MQ=255) tATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACCGCGAACCGTGGGCCTGAGCCGCGGCAACCCA‑GGCGTCGCAGACGGTTGGCAAATGTCGTACGGAGGGCacaaga > 1:523844/1‑138 (MQ=255) gcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCGCCTCTATTTCGGGGGGCATCTGACCTggct > 1:375732/1‑141 (MQ=255) taAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGAc < 1:115142/143‑1 (MQ=255) taAAGCGTCTTAGCAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGAc < 2:261569/143‑1 (MQ=255) aaTCGGCTTAACAACCCTGGCCCGGTACACAT‑GG‑TAGCGGTCCAACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCg < 2:642324/143‑1 (MQ=255) gTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCACATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGt > 1:662512/1‑143 (MQ=255) acaacCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCATGGGCCTGAGAACCGGC‑CCATATTGAGTATTTTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAAc > 1:668831/1‑143 (MQ=255) ccTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGCACCGCGGGCGTGCGCTCAGGCAACACA‑GGAGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaa > 1:381909/1‑143 (MQ=255) ccTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTTGGCCGAAGACGCGGCAACACA‑CGCGTCGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGGCTAGTGACCGGCGACTGAACGGCaa > 2:322180/1‑143 (MQ=255) gCCCGGTA‑ACAT‑GTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTCCGTGGCGCATATGACATGGAATATAGACCGACGACTGAACGGCAAAAAc > 1:823/1‑143 (MQ=255) gCCCGGTA‑ACAT‑GTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAAAACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGACCGGCAAAAAc > 2:844859/1‑143 (MQ=255) cccGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACa > 2:210204/1‑143 (MQ=255) ccGGTA‑ACAT‑GTGTACCGGGCCTACAACGAAAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATAGAGTTGGCACATGTAGTACGGGGGACATCTCAACTGGAATATAGACCGGCGACAGAGCGGCAGAAGCaa > 2:226343/1‑143 (MQ=255) tgtgcacaT‑GG‑TACCGGGCCTCCAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaaa < 1:237099/139‑1 (MQ=255) | GTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAT‑GT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACA‑GGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAA > NC_000913/1868722‑1868997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |