| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,715,257 | Δ1 bp | 12.5% | coding (64/882 nt) | yfiE ← | putative LysR‑type DNA‑binding transcriptional regulator YfiE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,715,257 | 0 | A | . | 12.5% | 126.6 / 15.5 | 48 | coding (64/882 nt) | yfiE | putative LysR‑type DNA‑binding transcriptional regulator YfiE |
| Reads supporting (aligned to +/- strand): ref base A (30/10); new base . (4/2); total (35/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01 | |||||||||||
CAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTC > NC_000913/2715126‑2715399 | cagcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAGCT‑TCGCaaaa < 2:238303/143‑1 (MQ=255) agcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCG‑AGCTTCGCaaaaa < 2:217854/143‑1 (MQ=255) aaCTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAACC‑TCGCAAAATGGa > 1:767592/1‑143 (MQ=255) aCTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCG‑AGCTTCGCaaaaattgt < 1:425000/143‑5 (MQ=255) tCCTTCGCGGGTAAGGCACACCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCtt < 1:915953/143‑1 (MQ=255) ttCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAGCAATCCTGCTCAAGCTGGTGGATATGACAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAGAGGAGCCCTCGTcca > 1:53845/1‑143 (MQ=255) gggTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACg < 1:526960/143‑1 (MQ=255) gggTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACg < 1:526923/143‑1 (MQ=255) acaTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCACATAGGAACCCTCTTCCTCCTCGGTTTTTAGc > 1:346448/1‑143 (MQ=255) aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAGAGGAACCCTCTTCCACCACGGCTTTAAGCGt > 2:97245/1‑143 (MQ=255) tCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGATTACAAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGta < 1:908975/143‑1 (MQ=255) cAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCCCACAAAGGAACCCTCTTCCACCACGGTTTTAAGACTAATAAAACga > 1:788685/1‑142 (MQ=255) aaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGGAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATccg > 1:803111/1‑142 (MQ=255) aaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCa > 1:588068/1‑143 (MQ=255) aaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGACGC‑TCGCAACAAGGAACCCTCCTCCACCACGGTTTTAAGCGTAATAACACGGCGCAGATCCac > 1:258972/1‑142 (MQ=255) aTAACTGCACTGAAACCTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATa < 1:679551/143‑1 (MQ=255) cTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAAccc > 2:78444/1‑143 (MQ=255) tGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAACAGGAACCCTCTTCCACCACGGTTTTACGCGTAATAAAACGGCGCAGAGCCACAAcccc > 2:192700/1‑143 (MQ=255) tGAAAACTCCTGCTCAAGCTGCTGAATATGAACAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGAAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGACGCAGATCAATAACCCCAAAcc > 1:320944/1‑143 (MQ=255) tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAAcc > 1:464074/1‑143 (MQ=255) gAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCt < 1:633595/143‑1 (MQ=255) aaaaCTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCAACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTa > 1:786918/1‑143 (MQ=255) tCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACATCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAAATCCATAACCCCAAACCTATCGaa > 1:715994/1‑143 (MQ=255) tCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGTAAAAAGGAACCCTCTTCCACCACGGTTTTCAGCGTACTAACACGGCGCAGATCCATACCGCCAAAACCATCaaa > 1:750919/1‑143 (MQ=255) tGCTCAAGCGGCTGAATATGAACAGTCACCGTCGATTGCGTACAGCCCAATTTTTGCGTAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAat < 2:438010/143‑1 (MQ=255) cTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAatat < 1:721623/143‑1 (MQ=255) cTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGATTTAAGCGTAATAAAACGGCGCAGATCCATAACCCGAAACCTCTCGAAAatat > 2:628845/1‑143 (MQ=255) tCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATc > 1:675588/1‑143 (MQ=255) cAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCg > 1:221973/1‑143 (MQ=255) tgctgAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATCAACCACAAGATATCCATAACCCCAAACCTATCGAAAACATCGAATca > 2:886481/1‑142 (MQ=255) ctgAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCGACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAAAATCGAATCGAg > 1:545816/1‑143 (MQ=255) aaTATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCATCGTCCGCAACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTAGCGAAGATATCGAATCTAGAat > 2:493397/1‑143 (MQ=255) tGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATaa > 2:265784/1‑143 (MQ=255) gAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAACTATCGAATCTAGAATATaaa > 1:5886/1‑143 (MQ=255) aaaGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGGTTTAAGCGTAATAAAACGGCGCCGATCCATAACCCCAAACCTCTCGAAAAAATCGAATCTACAATATaaaaa > 2:313484/1‑143 (MQ=255) aaGTCACCGTCGATTGTGTACAGCACAATTTTAGCGAAGC‑TCGCAAGAGGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAAc < 1:75042/143‑1 (MQ=255) tCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGTACATATCGAATCTAGAACACAAAAAcatt > 1:809819/1‑143 (MQ=255) tCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCAtttt > 1:563748/1‑143 (MQ=255) ttGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTGATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATCTTTTTa > 1:521068/1‑143 (MQ=255) gtgtACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACTGCTCATATCCATAACCCCAAACCTATCGAAAATAGCGAATCTAGAATATAAAAACATTCATTTTTTTaaa < 1:105615/143‑1 (MQ=255) cacaATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTACACTACAGTTTTAAGCGTAATAAAACGGCGCAGATACATAACCCCAAAACTATCGAAAATATCGAAGTGAGAATATAAACACAAACATTTTATTAAATCTTCCgt > 2:239368/1‑143 (MQ=255) aaTTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTc > 1:244176/1‑143 (MQ=255) aaTTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTc > 1:843069/1‑143 (MQ=255) aaTTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCCATCCAAAACATCGAATCAAGAATATAAACACATTCCTTTTCTAAAATGTTCCatggc > 2:668028/1‑141 (MQ=255) tttttGCG‑AGCTTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCgg > 1:683239/1‑143 (MQ=255) ttttGCG‑AGCTTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACTCCAAACCTATCGAAAATATCGAATCTAGAATATAACAACATTCCTTTTTTTAAATGTTCCGTCTCggg > 2:291295/1‑143 (MQ=255) ttttGCG‑AGCTTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATCAAAACACTCATTTTTTTAAATGTTCCGGGTCggg > 2:769731/1‑143 (MQ=255) ttttGCG‑AGCTTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCggg > 1:713597/1‑143 (MQ=255) aaGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATAGAAAATAGAATTTAAAAACATACATATTTTTAAATGTTCCGTGTCGGGTACTGTc > 1:291062/1‑143 (MQ=255) | CAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTC > NC_000913/2715126‑2715399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |