Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,500 Δ1 bp 12.8% intergenic (‑176/+171) yjhU ← / ← yjhF KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU/KpLE2 phage‑like element; putative transporter YjhF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5000A.12.8% 102.2 / 15.1 47intergenic (‑176/+171)yjhU/yjhFKpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU/KpLE2 phage‑like element; putative transporter YjhF
Reads supporting (aligned to +/- strand):  ref base A (18/23);  new base . (4/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-02

CCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAA  >  NC_000913/4520367‑4520640
                                                                                                                                     |                                                                                                                                             
ccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCGGCTTGTCCCCCATACTGTAAAGCCGGAGACGTG‑CTACg                                                                                                                                     >  1:460795/1‑143 (MQ=255)
ccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCCTACTGTAGAGCCGGAGACATG‑CTCCg                                                                                                                                     >  1:348614/1‑143 (MQ=255)
ccTGTTTACACGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCg                                                                                                                                     <  2:334649/143‑1 (MQ=255)
 cTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATGTTTTCTGCCTCTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCgg                                                                                                                                    <  1:665160/143‑1 (MQ=255)
  tGTTTACCCGGCGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGc                                                                                                                                   <  2:234590/143‑1 (MQ=255)
   gTTTACCGGGGGAGTGGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCt                                                                                                                                  <  2:899114/143‑1 (MQ=255)
   gTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt                                                                                                                                  <  1:521258/143‑1 (MQ=255)
           ggAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGCTCGCCCGCTGTTCCCCCATACTATAAAGCGAGAAACATT‑ATCCGGCTTTACAGTa                                                                                                                          >  1:214553/1‑143 (MQ=255)
               agTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGCGCGATCGCACGCGGTTCCCCCATACTCTAAAGCCGCAGACATG‑CTCCGGCTTCACACTactgc                                                                                                                      >  2:544886/1‑139 (MQ=255)
                 ttGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTg                                                                                                                    >  2:644428/1‑143 (MQ=255)
                    aTGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGCGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTAATgaca                                                                                                                 >  2:697063/1‑141 (MQ=255)
                            tGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGCGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAccc                                                                                                         <  1:126246/143‑1 (MQ=255)
                                      aTGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATa                                                                                               <  2:466908/143‑1 (MQ=255)
                                       agATTTCCTACAGATATTTTATTCTTTTACCTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCAGACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATaa                                                                                              <  2:183932/142‑1 (MQ=255)
                                            ttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                                                         <  1:723875/143‑1 (MQ=255)
                                                aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCCTACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGtt                                                                                     >  2:523421/1‑143 (MQ=255)
                                                  aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTGCGGCTTTACAGTATTACTGAGAAAACACCCCTCAAGCATAATCGacgactca                                                                                   >  2:20477/1‑136 (MQ=255)
                                                   gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCaa                                                                                  <  1:92471/143‑1 (MQ=255)
                                                     tatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTATTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTCCAGTATTACTGAGAATACACCCATCAAGCATAATCGAGGGTTCAata                                                                                >  2:874125/1‑143 (MQ=255)
                                                       ttttATTCCTTTACATGAAACAAACCAGCATACCATTTGGGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATc                                                                              <  1:837995/143‑1 (MQ=255)
                                                        tttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCg                                                                             <  1:120144/143‑1 (MQ=255)
                                                           aTTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCGAGCATAGTCGAAGGTTCAATATCGccc                                                                          >  1:354255/1‑143 (MQ=255)
                                                                     aTGAAATAACCCAGCTTCCTATTCTAGCGATCGCCCTCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAATGTTCAATATCGCCCCTTTTTGATc                                                                <  2:662040/143‑1 (MQ=255)
                                                                        aaaTAAACCAGCGTACCATTTGGGCGCTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGcc                                                             <  2:413069/143‑1 (MQ=255)
                                                                         aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGCAGACACCCATCGAGCGTCATCGAAGGTTCAATACCGCCCGCCTTTGGTCGCCa                                                            >  2:485698/1‑143 (MQ=255)
                                                                         aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGACGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGCAGGTTCAATAGCGCCCCTTTTTGATCGCCa                                                            >  2:404401/1‑143 (MQ=255)
                                                                                cAGCATATTTTTTTAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                                                     <  1:403739/143‑1 (MQ=255)
                                                                                      aCCATTTGAGCGTTCCCCCGCTGCTCCCCCATTCTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGATAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTTATCGCCAGCGATTCGTCAcc                                               <  2:376265/143‑1 (MQ=255)
                                                                                             gAGCGTTCGCCTGCTGTTCCCCCATACTGTAAAGCCGGAGACATT‑CTCCGGCTTTACAGTATTACTGAGAATACACCCTTCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGa                                        <  2:70180/143‑1 (MQ=255)
                                                                                                cGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTCTTTGATCGACATGGATTCGTCACCGTGCCGACgg                                     >  2:611178/1‑143 (MQ=255)
                                                                                                 gATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGGACGTGGGATGTCGCCCCTTTTTGATCGCCAGCGCTTCGTCACCGTGCCGACGGt                                    >  1:906436/1‑143 (MQ=255)
                                                                                                  aTCGCCGGTTTTTCCCCCATACTGTATAGCCTGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCTTGAAGCATAATCGAAGGTTCAATAGCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGtt                                   <  2:457966/143‑1 (MQ=255)
                                                                                                  aTCGCCCGCGGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCGTTACAGGATTACTGAGAATACACCCATCAAGCATAAGCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGtt                                   <  1:95657/143‑1 (MQ=255)
                                                                                                   tCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTg                                  <  2:201072/143‑1 (MQ=255)
                                                                                                     tcccTCTTTTCCCCTATACTGTAAAGCCGGACACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGcc                                <  2:788747/142‑1 (MQ=255)
                                                                                                       ccGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCCGACGGTTGCCGt                              >  1:720432/1‑143 (MQ=255)
                                                                                                       ccGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGt                              <  1:748998/143‑1 (MQ=255)
                                                                                                         gCTGGTCCCCCATTCTGTAAAGCAGGAGACATG‑CTCCGGCTTTACAGTATTACTTAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtca                            <  2:910607/143‑1 (MQ=255)
                                                                                                                  ccATACTGTAAAACCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGAGTCAATATCGCCCCTTTTTGATCGCCAGCGGTACGTCACCGTGCGGACGGTTGCCGTCCCGCAATaaa                   >  1:17338/1‑143 (MQ=255)
                                                                                                                       cTGTCAAGCCGGAGACATG‑CCGCGGCTGTACGGTAGTACGGAGGATACACCCATCAAGCATAGTCGCCGGGTCAGGGTCGCGTCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg              <  2:796211/143‑1 (MQ=255)
                                                                                                                            aaGCCGGAGACGTG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAATGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGa         <  2:119930/143‑1 (MQ=255)
                                                                                                                            aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGCATACCCCCATCAAGCATAATCGAAGTATCAATATCGCACCTTTATGATCGCCAGCGATTCGTCACCTTGCAGACGGCTACAGTCACTCACTAAATTAtacgata         >  2:32464/1‑137 (MQ=255)
                                                                                                                            aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACACAATAAAATATAGAGGa         >  1:8122/1‑143 (MQ=255)
                                                                                                                             aGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATACCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGAc        >  1:855151/1‑143 (MQ=255)
                                                                                                                             aGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCGATATCGCCCCTTTTTGCTCGCCAGCGCTTCGTCACCCTGCAGACTGTTGCCGTCACTTACTAAATTATGGTGGaa        >  1:320501/1‑142 (MQ=255)
                                                                                                                             aGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGAc        >  1:864706/1‑143 (MQ=255)
                                                                                                                                cGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGCGGGGCAgg     >  2:180687/1‑142 (MQ=255)
                                                                                                                                   agaCATG‑CTCGGGCTTTGCAGGATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCGTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaa  <  1:592786/143‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                             
CCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAA  >  NC_000913/4520367‑4520640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑