| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 725,279 | T→C | 10.5% | K586K (AAA→AAG) | kdpB ← | K(+) transporting P‑type ATPase subunit KdpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 725,279 | 0 | T | C | 10.5% | 103.3 / 4.0 | 47 | K586K (AAA→AAG) | kdpB | K(+) transporting P‑type ATPase subunit KdpB |
| Reads supporting (aligned to +/- strand): ref base T (14/25); new base C (3/2); total (17/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01 | |||||||||||
GACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTCGCCGC > NC_000913/725150‑725416 | gACGATAGTCGAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGt < 1:784813/143‑1 (MQ=255) gagagtCAGGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCAGTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTgg < 2:180231/138‑1 (MQ=255) gATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGTAGTATTTCGCCACATCggcgg > 1:107765/1‑139 (MQ=255) tAATCGTGGCGATGAAGAGCACCGCACTGAGGATTGCGGAGTCGGGCAAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCa < 1:118787/143‑1 (MQ=255) ttCAGGGCGTGGAAAATCACCGCGCTCAGGATTGTGGACAGCAGGTTGTGCGTGGACATGATGTTCAGCGCATTCAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAAt < 2:786915/142‑1 (MQ=255) aTCAGGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATACAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAAt < 2:615795/143‑1 (MQ=255) atagAAAATCACAGCACTGAGGAATGCAGAGACGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGCTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAgg < 1:16452/140‑1 (MQ=255) gAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCCTTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTCTTTCGCCCCATCGTCGCCAATGCGGAAggtgg > 2:101026/1‑143 (MQ=255) gaTCACCGCACTGAGGAGTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCa < 2:171423/142‑1 (MQ=255) tCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATGTCGCCACATCGGTGGCAATGGTGACGGTGGTCAGc > 1:428647/1‑143 (MQ=255) cgcgtaCTGAGGACTGCGGAGTCGGGCGAATGCAGCCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGGCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGa < 2:71293/138‑1 (MQ=255) gATTGCGGAGGCGGGCAAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCGGAAGGTGGTCAGCGAGCCACGGGtca < 1:878227/143‑1 (MQ=255) ttGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGtcatc < 1:189717/143‑1 (MQ=255) gggCGGAGTCGGGCGAATGCGGGCACATGATGTTCAGCGCATTTAACTGCGGATCCCAAAACTTTGATGTCGCTGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGtcatc < 1:909022/141‑1 (MQ=255) cgaaggcGGGCGAATGCAGGCACATGACGGTCAGCGCATGTAACTGCGGGCACGGTGCCGCGAATGCGGCCGGAAGAATGGCGCAGTATGGCGCCACAGCGCTGGCAAAGCTGACTGCGGTCAGCAAGCCAAGGGCCCTCAGc > 2:758319/7‑143 (MQ=255) gacGGGCGAAGGCGGGCACAGGAGGGTCAGCGCAGGTAAATGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCt < 1:255680/141‑1 (MQ=255) ggCGAATGCAGGCACATGATGTTCGGCGCATTTAACTGCGGATATGTTGCCGCGAATGTCGCCGGAATACTGGCGACGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCCAGCCACGGGTCATCAGCATCTGttt < 2:48258/143‑1 (MQ=255) ggCGAATGCAGGCACATGATGTGCAGCGCATTTAACTGCGGATACGTTGCCTCGAATTCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGttt < 2:359733/143‑1 (MQ=255) ggAATGCATGCACATGATGTCCAACGCATTAAACTGCGAATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGc < 2:915269/142‑1 (MQ=255) aTGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCac > 2:858092/1‑142 (MQ=255) cAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAAtgt < 1:311375/143‑1 (MQ=255) gggacaTGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGGATTGCGCCACGTGGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGc < 2:93951/140‑1 (MQ=255) cgcatgTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTCGTCAGCGAGCCACGGGTCATCAGCATCTGCTTGCCAATGTGcaccac < 1:427516/140‑1 (MQ=255) ggatgTTAAGCGCATTGAACTGCGGATACGTGGCCGCCAATGCCGCCGGAACAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCaccacc < 1:310142/142‑1 (MQ=255) gatgTTCGGCGCAGTTAAGTGCGGATACGGTGCGGCTAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTGAGCGAGCCAGGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCt < 1:130768/143‑1 (MQ=255) gatgTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATACTGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCCTCTGTTTGCCAATGGGCACCAGCt > 2:915262/1‑143 (MQ=255) gatgTTCAGCGCATTTAACTGCGGATACGTTGCAGCGCATGCCACCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCCCGGGTCAGCAGCAGCTGGTGGCCAATGTGCACCACCt > 2:745303/1‑143 (MQ=255) tgTTCAGCGTATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGGGAGGGAGCAGGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCg < 1:909214/143‑1 (MQ=255) ttCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAATACTTCGCcatt > 1:160479/1‑67 (MQ=255) ttCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAATACTTCGCcatt < 2:160479/69‑3 (MQ=38) cTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGt > 1:89341/1‑143 (MQ=255) ttGCCGCTAATGTCTCCGGAGTCGTGGCGATGTTCTTCTCGGCACCGTTGGCAATGCTGAAGGTGGCCAGCGATGCTCCGGTCACTAGCTTCTGTGTGCCAATGTGCACCACCTCGATCCACTTGGTCGGGTTAGAGTCgaga < 2:796179/143‑1 (MQ=255) tGCCGCGACTGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGAt < 1:27958/143‑1 (MQ=255) tGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGAt < 2:834012/143‑1 (MQ=255) ccGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCg > 1:130810/1‑143 (MQ=255) cgcgAAGGCCTCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGa < 2:426572/143‑1 (MQ=255) gcgAATGCCGCCGGAATCATGGCGAAGTAGTTCGCCACATCGTTCGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGAc < 2:628437/143‑1 (MQ=255) cgccgGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGc > 1:89465/1‑143 (MQ=255) cccttattaatGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTAAGCGAGCCACGGGGCATCAGCATCGGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGcc < 1:24907/136‑1 (MQ=255) ctgAATAATGGCGAATGAGCTCGCCACATCGTTGGCCTTGCAATATGTGCTCGTCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCg < 2:399859/141‑1 (MQ=255) cgGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACATGGTCGGGTTAGAGTCGAGGACGACGATAATGccca > 2:2835/1‑142 (MQ=255) atgtGGCGAAATACTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCGGTTTGCCAATGTGCACCGCCTCGAGCGACTTGGTCGGGTTAGAGTCGAGCTCGGCGATATTGCCCGCctct > 2:692046/4‑143 (MQ=255) atGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTACCGTCGAGATCGACCATATTGCCCGCCTCttt > 2:638392/1‑143 (MQ=255) tGGCGAAATACTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGCCCCTATTGCCCGCCTCTTTc > 1:772433/1‑143 (MQ=255) cGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTcgcc < 1:567218/143‑1 (MQ=255) aaGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTcgccgc > 1:222647/1‑143 (MQ=255) aaGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTcgccgc > 1:691772/1‑143 (MQ=255) | GACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTCGCCGC > NC_000913/725150‑725416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |