Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	164.2 / NA	55	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/22);  total (33/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754309‑754580                                                                                                                                          |                                                                                                                                       cGCAGGACGAGGATGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGtttt                                                                                                                                   <  2:183443/143‑1 (MQ=255)           ggTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGCTGAGGGTGGGTTTTGCTTTTGtct                                                                                                                         >  2:299630/1‑141 (MQ=255)            gTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATc                                                                                                                        >  1:774494/1‑143 (MQ=255)              tgaagtgcagCCTGGGTCAAACGTGAACACACCTTTTGTACCGAGTGTACGGATATCAATATCGTCTGGCTCGATCCGCCCTTTCCGTGCGTTTTCGTTAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAg                                                                                                                      <  1:421836/143‑1 (MQ=255)              tgaagtgaagGCTGAGTAAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACTTCTTGACCCAGCGTGCCTTTCAGTACATCTAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAg                                                                                                                      <  2:292703/143‑1 (MQ=255)                aagtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCCGTGAGGGTGGGGTTTGCTTTTGTATCAGcc                                                                                                                    >  2:570261/1‑143 (MQ=255)                  gtgaggCATTGGTCAAAGGTGAACACCCCTTGTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGGGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAt                                                                                                                  <  2:205010/143‑1 (MQ=255)                  gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAt                                                                                                                  <  2:21272/143‑1 (MQ=255)                    gaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGACTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGCTGACGGTGGGTTTTGCTTTTGTATCAGCCAttt                                                                                                                >  2:714796/1‑143 (MQ=255)                       gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                             <  2:517628/143‑1 (MQ=255)                       gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                             <  2:425157/143‑1 (MQ=255)                          tGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAgggc                                                                                                          >  1:310477/1‑141 (MQ=255)                          tGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGtc                                                                                                          >  1:652893/1‑143 (MQ=255)                            ggTCAAAGGTGAACACACCTTTGGAACCGAGAGTACGGATATCAATAACATCTTGCCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGtctc                                                                                                        <  2:598634/143‑1 (MQ=255)                             gTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTcc                                                                                                       <  1:592832/143‑1 (MQ=255)                             gTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTcc                                                                                                       <  1:599568/143‑1 (MQ=255)                                aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTa                                                                                                    <  1:905284/143‑1 (MQ=255)                                    gTGAACACACCTTTGCAACCGCGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAgcgc                                                                                                <  2:385206/143‑1 (MQ=255)                                       aaCACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCATCCTTTTAAGGTCTCCTTAGCGCCtt                                                                                             >  1:422861/1‑143 (MQ=255)                                          acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCCGCCATTTAAGGTCTCCTTAGCCCCATAtt                                                                                          >  1:330341/1‑143 (MQ=255)                                          acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                          <  1:576618/143‑1 (MQ=255)                                          acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                          >  1:720314/1‑143 (MQ=255)                                            acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTGTGTATCAGCCATTTAACGTCTCCTTAGCGCCTTagttc                                                                                        >  1:536596/1‑139 (MQ=255)                                             ccctttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCg                                                                                       <  1:149522/140‑1 (MQ=255)                                               ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTCTCAGCACATCCAGTTCAACAGCTGTATCCCCGATGCAGGCGGCTTTTGCTTCTGTATCAGCCATTTAAGGTCTCCCTAGCGCCTTATTCCGtc                                                                                     >  2:360463/1‑142 (MQ=255)                                                    aaCCGAGTGTACGGATATCAATAACATCTTGACCCAGCGTGCGTTTCAGCACATCCAGTTCAACAGCTGGATCCCAGGTGAGGGTGGGTTTTGCTTTTGTATCAGCCCTTTAAGGTCTCCTCAGCGCCTGATTGCGTGAGAcg                                                                                >  2:410759/1‑142 (MQ=255)                                                    aaCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTAGGTTTTGCTTTTGTATCAGCCATCTAAGGTGTCCGAAGCGCCTTATTGGGTGAGAcc                                                                                >  2:411935/1‑142 (MQ=255)                                                      ccGAGAGTACGGATATCAATAACATCTTGACCCCGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTCTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTCTGACTgg                                                                              >  1:27555/1‑142 (MQ=255)                                                      ccGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATACAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGc                                                                              >  1:453264/1‑143 (MQ=255)                                                      ccGAGAGTACGGATATCAATAACATCTTGACCCAACGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGAGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGc                                                                              >  1:314883/1‑143 (MQ=255)                                                        gagaGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCTTTGAGGGTCGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGTGTAAGACTgacc                                                                            >  2:37463/1‑140 (MQ=255)                                                              aCGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATCGCGTAAGACTGCCGGCAcgt                                                                      >  2:290227/1‑141 (MQ=255)                                                                  atatCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAt                                                                  <  1:582948/143‑1 (MQ=255)                                                                      gtttAACATTTTGTCCCAGTGTGCCTTTCAGCACATCCAGTTCAGTAGTTGTATTCCCGTTGAGGGTGGGTGTTGCTTTTGTATCAACCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGc                                                              <  2:223947/140‑1 (MQ=255)                                                                         tAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCtt                                                           <  2:592646/143‑1 (MQ=255)                                                                              tCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTACGACTGCCGGAACTTACACTTGCCTTCGcac                                                      >  1:787277/1‑143 (MQ=255)                                                                                  gACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATcg                                                  >  2:302691/1‑142 (MQ=255)                                                                                     ccAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAAcc                                               >  1:501038/1‑143 (MQ=255)                                                                                       aGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGGCTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTg                                             <  1:690036/143‑1 (MQ=255)                                                                                        gCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTgg                                            >  2:774926/1‑143 (MQ=255)                                                                                         cGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAATTTAAATTTGCCTTCGCACATCAACCTGGc                                           >  1:404453/1‑143 (MQ=255)                                                                                           tGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCCGAACTTAAATTTGCCTTCGCACATCAACCTGGCtt                                         >  2:250910/1‑143 (MQ=255)                                                                                            gCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCACGCttt                                        >  2:263109/1‑143 (MQ=255)                                                                                              cTTTCAGCACATCCAGTTCAACAGCTGTATCGCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTGAAAGTGGCCTCCGCACATCAACCTGGcagtac                                      >  2:135036/1‑138 (MQ=255)                                                                                              cTTTCAGCACATCCAGTTCAACAGCTGTATCCGCGGTGAGGGGGGGTTATGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAc                                      <  1:290227/143‑1 (MQ=255)                                                                                              cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGTCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAc                                      >  1:793225/1‑143 (MQ=255)                                                                                                    gCACATCCAGTTCAACAGCTGTATCCCTGTTGAGGGTGGTTTTTGTTTTTGTATCAGCTATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                <  2:137208/143‑1 (MQ=255)                                                                                                    gCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACGCGttt                                >  1:351415/1‑143 (MQ=255)                                                                                                                 aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTCAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcg                   >  2:807881/1‑143 (MQ=255)                                                                                                                 aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcg                   <  1:731894/143‑1 (MQ=255)                                                                                                                 aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCATTACACGTTATTTATATGGCTcg                   >  1:446044/1‑143 (MQ=255)                                                                                                                    aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCATCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccg                <  1:299630/143‑1 (MQ=255)                                                                                                                    aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccg                >  1:900105/1‑143 (MQ=255)                                                                                                                         tATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTCTTTTATTTGGCTCGCCGCTCtg           >  1:71896/1‑143 (MQ=255)                                                                                                                              ccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaaa      >  1:526592/1‑143 (MQ=255)                                                                                                                                  tgggggtgggtTTTGCTTGTGTATCAGCCATTTAGGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAGCCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg  <  2:744002/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754309‑754580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑