Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	760,485	G→A	100%	G594S (GGC→AGC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	760,485	0	G	A	100.0%	136.7 / ‑6.7	55	G594S (GGC→AGC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (30/24);  minor base C (1/0);  total (31/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC  >  NC_000913/760351‑760624                                                                                                                                       |                                                                                                                                             ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCg                                                                                                                                      >  1:776004/1‑143 (MQ=255)     gaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaca                                                                                                                                  >  2:133486/1‑141 (MQ=255)           gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCGGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCg                                                                                                                           >  1:516537/1‑143 (MQ=255)           gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCTTTGAGGAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCg                                                                                                                           >  2:886288/1‑143 (MQ=255)            ggcCTGCAGGAGCGGGCGAAACGCATCAGCACGGTGCCGGAAGCAGGTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGAGCGGCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGc                                                                                                                          <  2:722264/142‑1 (MQ=255)              ccTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGCTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCtt                                                                                                                        >  2:132422/1‑143 (MQ=255)                 gCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACg                                                                                                                     <  2:699297/143‑1 (MQ=255)                  cAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGGTTTATGGCGATCGCCAGGCGATGGCTGGCGGTGAGAAAGTGGTCGCCTGGAGCGGTGCGGAAAAC‑CCCGCTAACGc                                                                                                                    >  2:101071/1‑143 (MQ=255)                  cAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGc                                                                                                                    <  2:697372/143‑1 (MQ=255)                     gAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGACCGGTGCGGTAAAC‑CTCGCTTACGCCAc                                                                                                                 >  1:483811/1‑143 (MQ=255)                        cTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAG‑CTCGCTTACGCCAGGCt                                                                                                              >  2:906594/1‑143 (MQ=255)                         tGGCGAGACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTg                                                                                                             <  1:752947/143‑1 (MQ=255)                            ggataCGCATCAGCACGGTGCCGGAAGGAGTTGAGATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGtt                                                                                                          <  2:388473/139‑1 (MQ=255)                              aaaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTtga                                                                                                        >  1:900261/1‑143 (MQ=255)                                  gCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACAGTACGACCGTAGTTGTGTTTAAAAC‑CTCGCTTACGCCACGCTGGTTGATGaa                                                                                                    <  1:172532/143‑1 (MQ=255)                                   cATCAGCACGGGGCCGGAAGCGGTTGACAGGCAGCCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAGC‑CTCGCTTACGCCACGCTGGTTGATGAAg                                                                                                   <  1:470581/143‑1 (MQ=255)                                     tCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGc                                                                                                 >  1:236098/1‑143 (MQ=255)                                      cAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCa                                                                                                >  1:759862/1‑143 (MQ=255)                                        gCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGGTTTATAGCGATGGCGAGGCGATGGGTGCCGGTGAGAAACTGTTCGACTCGAGCGGTCCGGAAAAC‑CTCGCATACGCCACGCGGCTTGaggcaggcgct                                                                                              >  2:344138/1‑140 (MQ=255)                                        gCACGGTGCCGGAAGCAGTTGAAATGCAGTCACGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CACGCGTACGCCACGCAGGTCGAGGGAGGCAtt                                                                                              >  2:380531/1‑143 (MQ=255)                                            ggTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACACTGGTTGATGAAGGCATTCCgg                                                                                          >  1:424402/1‑143 (MQ=255)                                                ccGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCg                                                                                      >  2:168899/1‑143 (MQ=255)                                                 gggAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTGATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc                                                                                     <  1:848979/142‑1 (MQ=255)                                                         gTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCggg                                                                             >  2:440082/1‑143 (MQ=255)                                                          ttGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACGCATCCGTATG‑CGCTCGGGTCGATTGAGGCATTCCGGTTAGCCTGTAGGGt                                                                            >  2:786840/1‑143 (MQ=255)                                                          ttGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGt                                                                            <  1:886288/143‑1 (MQ=255)                                                                tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGAc                                                                      >  2:280599/1‑143 (MQ=255)                                                                tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGAc                                                                      >  1:192695/1‑143 (MQ=255)                                                                     tgtggcgTTGCCAAGAGTGAGGGCGATCGCCAGGCGATGGCGTCCGTTGAGAAACTGTTCGACTGGAGCGGGGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCgg                                                                 <  1:512327/139‑1 (MQ=255)                                                                     tctcGCGTTGCCAAGATTTCTGGCGATCGCCAGGCGATGGCTGGCGGTGAGAAACTGTTGGACTGGCGCGGCGCGGGGAGC‑CTCGCTGACGCCACGCTGGTTGCTGAAGGCAGTCCGGTTGGCCTGCCGGGTGCAgcggccgg                                                                 >  2:64417/1‑136 (MQ=255)                                                                     tctcGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGGGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCgg                                                                 >  2:11358/1‑143 (MQ=255)                                                                     tctcGCCTGGCCAAGATTTATGGCGATCGCCAGGCGCTGGCTGCCGGCGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCgg                                                                 <  1:7254/143‑1 (MQ=255)                                                                        cgcgTTGCCCGGATTTAGGGCGGTCGCCAGGCGGTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAATC‑CTCGCTTACGCCACGCTGGTTGATGAGGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcg                                                              <  2:734883/143‑1 (MQ=255)                                                                          cgTTGTCATGATTTAAGGTGTAAGCCAGGGGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgcg                                                            <  1:702880/143‑1 (MQ=255)                                                                             tGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTa                                                         <  2:89524/143‑1 (MQ=255)                                                                                cAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTCCct                                                      >  2:259754/1‑143 (MQ=255)                                                                                        aTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc                                              <  1:627535/143‑1 (MQ=255)                                                                                          agccaTCGCCTGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGGTTATGAAGGCATTCCGGTTCGCCTGTCGGGTGACGACTCCGGTCGCGGTACCTTCTTCCACCg                                            >  1:464539/5‑143 (MQ=255)                                                                                               tCGCCAGGCGCTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGCTCGCCTGTCGGGCGGAGACCCCGGTCGCGGTCCGTTCTTTCACCGCCAcg                                       >  1:60203/1‑143 (MQ=255)                                                                                                cGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgc                                      <  1:440082/143‑1 (MQ=255)                                                                                                     ggCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGa                                 <  1:133486/143‑1 (MQ=255)                                                                                                     ggCGATGGCTGCCGGTGAGAAACTGTTCGACGGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGCGGGAGGCATTCCGGTTCGCCTGCCGGGGAACGACTCCGGACGAGGCACCTTCTCCCCCCGCCCcgcgcgca                                 >  2:421271/1‑139 (MQ=255)                                                                                                       cggtGGCTGCCGGTGGGAAACTGTTCGTCGGGAGTGGTGCGGCAGTC‑CTCGCTTACGCCACGCTGGTTGATGAACGCCTTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATc                               <  2:435872/140‑1 (MQ=255)                                                                                                          cggcgcacGGTGAGAAACTGCTCGACTGGAGCGGTGCGGAACAC‑CTCGCCTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCAGCTACAGACTCCGGTCTCGGTACTTTCTTCCACCGCCACGCGGTGATCcac                            <  1:251598/136‑1 (MQ=255)                                                                                                                  ggTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGACGGCATTCCGCTTCGCCTGTCCGGTGCAGACTCCGGTCGCGGTACCTTCTTCCCCCGCCACGCGGTGGTCCCCACCCGGTc                    >  1:227087/1‑143 (MQ=255)                                                                                                                  ggTGAGAAACTGTTCGACTGAAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACATTCTTCGACCGCGCCGCGCTGCTCCACATCCTGtg                    >  2:181858/1‑142 (MQ=255)                                                                                                                            tGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTcc          <  1:280599/143‑1 (MQ=255)                                                                                                                            tGTTCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCAACGCGGTGATCCACAACCAGTCTAACGGTTcc          >  1:710181/1‑143 (MQ=255)                                                                                                                             gtgcggcTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGAGTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCa         <  2:127613/137‑1 (MQ=255)                                                                                                                              ttCGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCAc        >  1:467393/1‑143 (MQ=255)                                                                                                                              ttCGACTGGAGCGGTGCGGAAAAC‑CTCGCGTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCAc        <  2:192695/143‑1 (MQ=255)                                                                                                                                cGACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACtt      >  1:782590/1‑143 (MQ=255)                                                                                                                                 ggcgggggCGGTGCGGAGACC‑CTCGCTTACGCCACGCTGGGTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTa     <  2:286502/139‑1 (MQ=255)                                                                                                                                 gACTGGAGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTACGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCATCTTCCACCGCCACGGCCTGATCGACAACCCGTCTAACGGTGCAACTTa     >  2:630937/1‑143 (MQ=255)                                                                                                                                   cTGGAGCGGTGCGGAAAAG‑CTCGCTTACGCCACCCTGGTTGATGAAGGCATTCCGGTTCCCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTaca   <  2:245966/143‑1 (MQ=255)                                                                                                                                    tGGAGCGGTGCGGAAACC‑CGCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTacac  <  1:634875/143‑1 (MQ=255)                                                                                                                                       |                                                                                                                                             TTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAAC‑CTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC  >  NC_000913/760351‑760624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑