| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 915,081 | G→C | 12.5% | intergenic (‑224/+271) | lysO ← / ← aqpZ | L‑lysine exporter/water channel AqpZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 915,081 | 0 | G | C | 12.5% | 122.1 / 13.2 | 55 | intergenic (‑224/+271) | lysO/aqpZ | L‑lysine exporter/water channel AqpZ |
| Reads supporting (aligned to +/- strand): ref base G (29/19); new base C (5/2); total (34/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-01 | |||||||||||
AAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCC > NC_000913/914940‑915219 | aaaaGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGa > 1:843363/1‑143 (MQ=255) aGGAAATGTTAGGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgt < 1:642961/143‑1 (MQ=255) ggAAATGTTATACATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCTGAGGCGTACCGTGAAGGGCGGGGCAGCCAACATGGAtgtg > 2:766496/1‑143 (MQ=255) aaaTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTggg < 1:785038/143‑1 (MQ=255) aaggTTATGCATAAGGAGCCGTAGCGTAGTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGc < 1:110505/140‑1 (MQ=255) ttATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTCATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTgcg > 1:17147/1‑141 (MQ=255) aTAAGGTGCAGTAGAGTATTTGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAGCTGCACCGATGTTGGGGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGcgcg < 2:701219/143‑1 (MQ=255) aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGcgcg > 2:600048/1‑143 (MQ=255) aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAAGTGGGCTGAGGGcgcg > 2:331023/1‑143 (MQ=255) aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAAGTTCAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCCCAAGGCCGGGGCAGCCCACATGGCTGTGGGCTGACGGcgcg > 2:392043/1‑143 (MQ=37) aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAACTTCAAACTGCACCGATGTTGGTCGCGCCAACAACGCCGAGGCGTTCCCTGAACGACGGGGCAGCCCACATGGATGTGGGCCGAGGCcgcg > 2:8874/1‑143 (MQ=37) tAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGGTGTGGGCCGAGTGcgcgg > 1:741555/1‑142 (MQ=255) ttaGTATATTATTCGTGATCAGTTAAAGATACTCTGGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACa < 1:49678/141‑1 (MQ=21) agtagtGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTGGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGACGGGGCAGCCCACATGGATGTGCGATGAGCGCGCGTTTTACAgg > 2:667754/1‑143 (MQ=25) gTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAggg > 2:139746/1‑143 (MQ=255) tAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGCTTTACAGCGa > 2:390693/1‑143 (MQ=255) agTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCCCCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGt < 1:885941/143‑1 (MQ=255) aaaGATATTCTTGCGCTTTAATTACACACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGANGAGGGCGGGGGGCGCGCTCTACAGGGATGTTACCGCGCGCCCgccac > 2:30106/1‑139 (MQ=18) ggtgatTCGTGCGCTTTAAGTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCgg < 1:62701/139‑1 (MQ=255) tatTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGCCCCGGTAg > 1:53694/1‑143 (MQ=255) tttAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAggggt > 1:491426/1‑141 (MQ=255) tttAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt > 1:896456/1‑143 (MQ=255) tttAATTACAAACTGCACCGATGTTGGTGGCGACAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAgggct > 2:476202/1‑141 (MQ=255) tAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATaa < 1:765394/143‑1 (MQ=255) tAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATaa < 2:41190/143‑1 (MQ=255) aTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGt > 2:654729/1‑143 (MQ=255) tACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCg > 1:184551/1‑143 (MQ=255) ccaaCTGCACCGCCGTCGGTGCCGTAAACAAAGCCGCGGCGTTCCGTCGCGGCCGCGACAGGATAAAAGCACGGGGGCTGAGGGCGCGTTTTACAGGCCAGTCAAAGCAGGGTTGACCCGGTAGCCGTAAGGGATAAGTCGAg < 2:693220/141‑1 (MQ=255) aaCTGCACGGATGTTGGTGGCGTCAAGATGGCCGAGGCGTCCGCTGAAGGGCGGGGGAGCCGACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTGACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAggg < 2:15794/143‑1 (MQ=14) tGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCAc > 1:18412/1‑143 (MQ=255) cACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGCGGGCACcg > 2:909880/1‑143 (MQ=255) aCCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACcgc > 2:879468/1‑143 (MQ=40) tggtggCGTCAAAACCGCCGAGGCGTTCCCCGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcggc < 1:183353/143‑1 (MQ=25) ggtggCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcggcg < 1:600048/143‑1 (MQ=35) cGTCAAAATCGCCGAGGCGTCCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAtttt < 1:269261/143‑1 (MQ=25) gTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTg > 1:351315/1‑143 (MQ=35) aaaaGCGCCGAGGCGTTCCCGGAAGGCCGGGGCAGCCAACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAGGTTACCTCGGGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTc < 2:337303/143‑1 (MQ=11) aGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGccc > 2:351031/1‑143 (MQ=32) ttCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGACTTTGTTCGCCAGAGCCCggggg > 2:460492/1‑143 (MQ=17) ccTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGGTGTTAACTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGc > 1:433260/1‑143 (MQ=17) ccTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGc > 1:698523/1‑143 (MQ=32) tGAAGGCCGGGGCAGCCCACATGGATGTGGGGTGAGGGCGCGTTTGACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGTACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAg < 1:624832/143‑1 (MQ=12) gcgccctcAGCCCACATCCATGTGGGCTGCGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggc > 1:800792/8‑143 (MQ=11) gcgccctcAGCCCACATCCATGTGGGCTGCGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggc > 1:321098/8‑143 (MQ=11) gCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCAATTTACAGGTGTGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATGTTGTTCGCCAGGGCCCGGGGGTGCAGGGggc > 1:469558/1‑143 (MQ=11) ccGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAggggggg > 1:651100/1‑141 (MQ=17) cGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcgg < 1:139746/143‑1 (MQ=32) ggggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggc < 1:343460/143‑1 (MQ=32) ggtcAGCCCACATGGGTGTGGGCTGAGGGCGCGTTTTACAGGGATGTTCCCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggcg < 2:207704/140‑1 (MQ=12) tcAGCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggcggc > 2:35556/2‑143 (MQ=12) tcAGCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggcggc > 1:510349/2‑143 (MQ=12) cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAtt < 1:103861/143‑1 (MQ=32) cccACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGGCAGAGCCCGGGGATGCAGGGGGCGGCGGCGAtt > 2:517036/1‑143 (MQ=255) ccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTGGCCGTAAGGGATAGGTCGAGGGCACCGCGCAGCGGCGATTTTGGTCGCCAGAGCCCGGGGGTGCGGGGGGCGGCGGCGATTg > 1:603141/1‑143 (MQ=255) ccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTg < 1:750669/143‑1 (MQ=32) cagggATGGGGGCTGAGGGCGCGTTTTACAGGGATGTTAGCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgcc < 1:730865/140‑1 (MQ=12) | AAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCC > NC_000913/914940‑915219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |