Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,026,350	A→G	8.7%	E627G (GAA→GGA)	helD →	DNA helicase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,026,350	0	A	G	8.7%	130.2 / 2.2	57	E627G (GAA→GGA)	helD	DNA helicase IV
Reads supporting (aligned to +/- strand):  ref base A (31/18);  new base G (3/2);  total (35/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAA  >  NC_000913/1026211‑1026492                                                                                                                                            |                                                                                                                                               gCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCCGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGCCGAGTATGGAaga                                                                                                                                             >  1:177535/1‑143 (MQ=255)    acacGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCTGCTGCGGCGCGGGAGTCTATTATGGAAGATgc                                                                                                                                          >  1:297361/1‑143 (MQ=255)     cacGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAgcggcg                                                                                                                                         >  1:97391/1‑143 (MQ=255)      acGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGgcgc                                                                                                                                        <  1:363869/143‑1 (MQ=255)      acGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGgcgc                                                                                                                                        >  1:481666/1‑143 (MQ=255)          gcgCCGAGGTTGCTAAGCGCCTTTATTACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATTGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACt                                                                                                                                    <  2:69626/141‑1 (MQ=255)            gCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGc                                                                                                                                  >  1:55219/1‑143 (MQ=255)                  ggtGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGGTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCgg                                                                                                                            <  2:313822/141‑1 (MQ=255)                  ggtGCAAATCGACGTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCAGCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCTGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCgg                                                                                                                            <  2:31822/141‑1 (MQ=255)                       aaaTCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGCAGAGATGGTTTTCCGGCTGCGGCGCGGGAGACGATGATGGCAGAGGCGCTACTGCCACCGGTGGAg                                                                                                                       >  2:97429/1‑143 (MQ=255)                       aaaTCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGGGTCGATGATGGGAGAGGCGCTACTGCCACCGGGTGAg                                                                                                                       >  2:583881/1‑143 (MQ=255)                       aaaTCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGCAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGGAGAGGCGCTACTGCCACCGGTTGAg                                                                                                                       >  2:587071/1‑143 (MQ=255)                                ttATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCAGCTGCCACCGGTTGAGGATTTCCCg                                                                                                              >  2:432418/1‑143 (MQ=255)                                    gACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGAGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACg                                                                                                          >  2:829276/1‑143 (MQ=255)                                            aTGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGAGTTCCCGGACGCTgaagaa                                                                                                  >  2:876318/1‑143 (MQ=255)                                               ccAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGCAGTGCTGGTTTTCCGGCTGCGGCGCGGGGGTCGATTATGGGAGAGGCGACACTGCGCGCGGTTGAGGCTTTCTCGGACGCTgcagcgccg                                                                                               >  2:298120/1‑138 (MQ=255)                                                           aaCAGGCGGATTTCGTCCTCAGCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGCTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTAc                                                                                   <  2:657778/143‑1 (MQ=255)                                                              aGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGCCGCTGAAGCAAGGCGGTTAATCTACGTg                                                                                >  1:546881/1‑143 (MQ=255)                                                              aGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTg                                                                                >  1:453437/1‑143 (MQ=255)                                                              aGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTg                                                                                >  1:410495/1‑143 (MQ=255)                                                                 cGGATTGCGTTAGCAGCGTGGGCTTGCAGGCGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGgcg                                                                             <  2:425239/143‑1 (MQ=255)                                                                  ggATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGgcgc                                                                            <  2:55219/143‑1 (MQ=255)                                                                     ttACGTCATGATCGTTGGCTGGCAGGAGGGAAGTGATGGTGTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGa                                                                         <  2:477759/143‑1 (MQ=255)                                                                       aCGTGATCATCGTGGGCTTGCAGGAGGGAAGGGGTGGTGTGCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGAcc                                                                       <  2:279831/143‑1 (MQ=255)                                                                        cttcatcaTCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGAGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATCTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGAccc                                                                      <  2:315362/141‑1 (MQ=255)                                                                            atcatcGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGc                                                                  >  1:487251/1‑143 (MQ=255)                                                                              catcGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGTCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCAc                                                                >  2:227327/1‑143 (MQ=255)                                                                                tcGTTGGCTTGCAGGAGGGAAGGGCTGGTTTCCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGc                                                              <  2:481666/143‑1 (MQ=255)                                                                                    tGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGAGTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGAGCACGCCATc                                                          >  2:703437/1‑143 (MQ=255)                                                                                     ggCTTGCGGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCg                                                         <  1:854739/143‑1 (MQ=255)                                                                                     ggCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATGATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCg                                                         <  2:519807/143‑1 (MQ=255)                                                                                      gCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGGCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGCAGAACAGCCGCTAATGTTCTTGGCGCTGACCCGGGCACGCCATCgg                                                        <  1:870235/143‑1 (MQ=255)                                                                                      gCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGGCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCCCTGACCCGGGCACGCCATCgg                                                        >  1:69785/1‑143 (MQ=255)                                                                                        ttGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGCGTCGAGTATGGAAGAGGCGCTACTGCCCCCGGCTGAGGATTTCCCGGACGCTGAAGACCTGCGGTTAATGTACGAGGCGCTGCCCCGGCCCCGCCACCCGGt                                                      >  2:365558/1‑143 (MQ=255)                                                                                        ttGCAGGAGGGAAGTGATGAATTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACAGCCACCGGTCGAGGATTGCCCGGACGCTGAAGAACGGAGGTAAACGTACGTGGGGCTGACCCGGGGACGCCATCGGGt                                                      >  2:100133/1‑143 (MQ=255)                                                                                              gaggGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATGATGGAAGAGGCGCTACTGCCACCGGTGGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTGCGTGGCGCTGGCCCGGGCACGCCATCGGGGATggtc                                                >  2:71045/1‑141 (MQ=255)                                                                                                 ggAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAAAGAACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGcgca                                             >  2:293892/1‑140 (MQ=255)                                                                                                    aGTGATGGTTTTGCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGtt                                          <  2:33776/143‑1 (MQ=255)                                                                                                     gTGATGGTTTTCCGGCCGCGGGGCGGCCGTCGACTACGGCAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACCCTGCAGAAAGGCCGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGttt                                         <  1:298120/143‑1 (MQ=255)                                                                                                       gATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGATGATGTTTTTCTGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTaa                                       >  1:419100/1‑143 (MQ=255)                                                                                                        aTGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGGTGAGGATTTCCCGGACGGTGAAGAACGGCGGCTAATGTACGTGGCGCCGACCCGGGCACGCCGTAGGGGATGGGCAgtgtgtcac                                      >  2:254043/1‑138 (MQ=255)                                                                                                            ttttCCGGGTGCGGCGCGGGGGTCGATTATGGGAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAag                                  <  1:583881/143‑1 (MQ=255)                                                                                                            ttttCCGGCTGCGGCGCGGGAGTCGATTATGGGAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAag                                  <  1:587071/143‑1 (MQ=255)                                                                                                              ttCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTGGTGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAagag                                >  1:464457/1‑143 (MQ=255)                                                                                                                         gcgcGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGCTAATGTACCTGGCGCTGAACCGGGCACGCCATCGGGGATGGGCACTGGTTAACACAGAGAATcccgcccc                     >  2:617525/1‑138 (MQ=255)                                                                                                                         gcgcGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGATACTGTGCGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTGACAAAGAGAATCCctctgc                     >  2:547452/1‑141 (MQ=255)                                                                                                                             gggAGTCGATTATGGCAGAGGCGCGACTGCCGCCGGTTGGGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCttt                 <  2:699327/143‑1 (MQ=255)                                                                                                                              ggAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGACTCCCTCTCCCTCtg                >  1:101303/1‑143 (MQ=255)                                                                                                                                aGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTtgtg              <  1:829276/143‑1 (MQ=255)                                                                                                                                    gATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTATGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGACTCCCTCTCCCTTTGTGGaaa          >  2:623620/1‑143 (MQ=255)                                                                                                                                    gATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAaga                                 >  2:294556/1‑120 (MQ=255)                                                                                                                                    gATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAaga                                 <  1:294556/120‑1 (MQ=255)                                                                                                                                     aTTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGacgt         >  1:508492/1‑140 (MQ=255)                                                                                                                                     aTTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAAt         <  1:876318/143‑1 (MQ=255)                                                                                                                                       tATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATAc       >  2:168158/1‑143 (MQ=255)                                                                                                                                        aTGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACt      >  1:518170/1‑143 (MQ=255)                                                                                                                                         tGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTg     >  2:184511/1‑143 (MQ=255)                                                                                                                                            aaGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAGCTACTGaag  >  1:277057/1‑142 (MQ=255)                                                                                                                                            aaGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATATTGaaa  <  2:410495/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                               GCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGAAGTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCTGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAA  >  NC_000913/1026211‑1026492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑