Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 85,882 G→A 7.6% G85S (GGC→AGC)  ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091385,8820GA 7.6% 137.7 / 3.4 53G85S (GGC→AGC) ilvIacetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base A (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-02

GCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGG  >  NC_000913/85740‑86023
                                                                                                                                              |                                                                                                                                             
gcgtACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGGGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTg                                                                                                                                               <  2:42930/140‑1 (MQ=255)
                aTTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTta                                                                                                                               >  1:138629/1‑143 (MQ=255)
                aTTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACAAATGCGATTACTGGCATCGCCACCGCTta                                                                                                                               >  2:57776/1‑143 (MQ=255)
                    aTCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCCATGCGGTTACTGGCATCGCCAACGCTTATATg                                                                                                                           >  2:106910/1‑143 (MQ=255)
                     tCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTTTATgg                                                                                                                          >  1:241001/1‑143 (MQ=255)
                     tCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATgg                                                                                                                          >  1:653411/1‑143 (MQ=255)
                      cATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGa                                                                                                                         <  1:472411/143‑1 (MQ=255)
                       aTGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTGCAGGGGCGACCAATGCGATTACTGGCATCGCCCCCGGTTATATGGat                                                                                                                        >  2:343056/1‑143 (MQ=255)
                         gTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGattc                                                                                                                      <  1:513790/143‑1 (MQ=255)
                           aTTAGTTCGGCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGattcca                                                                                                                    <  1:102626/143‑1 (MQ=255)
                            ttAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCCGGGGCGACCAATGCGATTACTGGCATCGCCCCCGCTTATATGGatggcct                                                                                                                   >  2:246228/1‑138 (MQ=255)
                             tAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGattccatt                                                                                                                  <  2:653411/143‑1 (MQ=255)
                              aGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGGCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGattccattc                                                                                                                 <  1:419724/143‑1 (MQ=255)
                                ctCGTCATGAGCGGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGAttccattcca                                                                                                               <  2:721705/142‑1 (MQ=255)
                                 tcgtcgTGAGCAGGCGGCGGGGCATATGGCCGCTGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATtccattccat                                                                                                              <  2:871618/143‑1 (MQ=255)
                                   gTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTa                                                                                                            <  1:516785/143‑1 (MQ=255)
                                    tCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGACGGGTCCAGGGGCGACAAATGCGATTACTGGCATCGCCACCGCTTATATGGAGTCCATTCCATTAg                                                                                                           >  1:569267/1‑143 (MQ=255)
                                          gCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCCTATATTGATTCACTTCCATTAGTTATcc                                                                                                     >  2:767570/1‑143 (MQ=255)
                                            aGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCGTCGCCACCGCTTATATGGGTTCCATTCCATTAGTTGTCCtt                                                                                                   >  1:474013/1‑143 (MQ=255)
                                              gcggcgGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCAATCCAAAAGTTGACCttta                                                                                                 >  1:241842/1‑142 (MQ=255)
                                                  tggTGCATATGGCGGATGGCCGGGCGGGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTGCTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCggg                                                                                             <  2:256773/142‑1 (MQ=255)
                                                  cggTGCGTCTGGCCGATGGGCTGGCGCGGGCGGCCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCggg                                                                                             <  2:824602/143‑1 (MQ=255)
                                                        atatGGCCGATGGCCTGGCGCACGCGAAAGGGGAAGTCGGCGTCGTGCTAGTAACGACGGGCACACGGGCGACAAATGCGATAACCGGCAACGCCAACGGGTATATGGACTCTATTCCACTCGGTGCACTGACCGAGCAGGTa                                                                                       >  2:909265/1‑143 (MQ=255)
                                                          atGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGAGTCCATTCCATTAGTTGTCCTTTCCGGGCAGGGAGc                                                                                     >  2:503282/1‑143 (MQ=255)
                                                                       tGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATa                                                                        >  2:213461/1‑143 (MQ=255)
                                                                       tGGCGCGCGCGACCGGGGAAGGCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTCGTTGTCCTTTCCGGGCAGGGGGCGAGCTGGTTGaca                                                                        >  1:751684/1‑141 (MQ=255)
                                                                          cgggcgcgGCCGGGGGTGTGGGGTTGGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGt                                                                     <  2:678870/140‑1 (MQ=255)
                                                                          cgcgcgcgACCGTGCAAGTCGGCGTCAGGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGt                                                                     <  1:141461/143‑1 (MQ=255)
                                                                          cgcgcgcgACCGGGGCAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGt                                                                     <  2:666096/143‑1 (MQ=255)
                                                                            cgggcgGCCGGGGGAGTCCGCGTCGTGGTGGTGACGGCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCGTGTATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTa                                                                   <  2:291034/140‑1 (MQ=255)
                                                                              cgcgACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGGCCAGGGGCGGCCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCGCGTTGATGGGTTACg                                                                 >  2:467650/1‑143 (MQ=255)
                                                                                    cGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTAGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTcc                                                                                                <  1:42672/106‑1 (MQ=255)
                                                                                    cGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTAGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTcc                                                                                                <  1:483376/106‑1 (MQ=255)
                                                                                    cGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTAGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTcc                                                                                                >  2:483376/1‑106 (MQ=255)
                                                                                    cGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTAGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTcc                                                                                                >  2:42672/1‑106 (MQ=255)
                                                                                      gggATGTCGGCGTGGTGCTCTTCACGGCGGGGCCTGAGCTTCCCCATGCGATTACTGGCATCGCGAGCTCTGATATGGATTCCATTCCATTAGTTGTCCTTTCCGTGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCttt                                                         <  2:680241/143‑1 (MQ=255)
                                                                                        gtggTCGGCGTCGTGCCGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCa                                                       <  1:613438/140‑1 (MQ=255)
                                                                                        gAAGTCGGCGTCGTGCTGGTAACGTCGCGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCa                                                       <  2:648871/143‑1 (MQ=255)
                                                                                                 gtcgtGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGCTGCCTTTCCGGAGTGCgt                                              >  1:273720/1‑142 (MQ=255)
                                                                                                  tcgtGGTGGTAACATCAGGCCCGGTGTCGACCTATGCGTGTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGAc                                             <  2:678993/143‑1 (MQ=255)
                                                                                                        tGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTAATGGCAGCGCCACCGCTTATATGGATTCCATTGCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAtggtg                                       <  1:650290/143‑1 (MQ=255)
                                                                                                          gTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACGTAGTggg                                     >  2:749942/1‑143 (MQ=255)
                                                                                                          gTAACCCCGGGTCCAGTGTCGACCAATGCGATTACTGGCATTGCCACCGCTTATATCGATTCCATTCCATTAGTTTTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTggg                                     <  1:318626/143‑1 (MQ=255)
                                                                                                            aaCGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGa                                   <  1:525381/143‑1 (MQ=255)
                                                                                                            aaCGTCGGGTCCAGCGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGCCCTTTCCGGGCAGCTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGa                                   <  1:6003/143‑1 (MQ=255)
                                                                                                                tCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGATACGATGCCTTTCAGGAGCGCGACCTGGTGGGGATTTc                               >  2:256597/1‑143 (MQ=255)
                                                                                                                  gggTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCCTTCGTTGTCCTTTCCGGGCAGGTACCGACCTCGTTGCTAGGTCAAGACGCCTTACAGGAGTGCGACAAGGTGGGGCTTTcgc                             >  2:53407/1‑143 (MQ=255)
                                                                                                                     tCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCCTTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGGCATGGCGGGGATTTCTCGAc                          >  1:792118/1‑143 (MQ=255)
                                                                                                                     tCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTAGGATGCATTTAAGGAGCGCGGCGTGGGGGGGGTTTcgcggc                          >  1:334570/1‑141 (MQ=255)
                                                                                                                       cAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTCTATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAAGGTGGGGATTTCGCGACCg                        >  2:752387/1‑143 (MQ=255)
                                                                                                                          tggCCACCAATGCGATTACTGGCACCGCCACCGCTTATATGGTTTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtg                     <  1:246228/142‑1 (MQ=255)
                                                                                                                                   aTGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGCCCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGCTTTCGCGACCGGCGGTGAAacac            >  2:905977/1‑143 (MQ=255)
                                                                                                                                    tGCGATTACTGGCATCGTCACCGCTTATATGGATTCAATTCAAGTAGGTATCGTTTCCGGGTGGCAAGCGACCTCGTTGATAGGCTATGATGCAGTTCAGCAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAAcaca           <  1:814021/143‑1 (MQ=255)
                                                                                                                                             tGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGCCATGGTGAGGATATCGCGACCGGTGGTTAAAAACAGTTTTCCgg  >  2:670252/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                             
GCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGG  >  NC_000913/85740‑86023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑