| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,856,333 | T→G | 10.6% | E199A (GAG→GCG) ‡ | ydjH ← | putative sugar kinase YdjH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,856,333 | 0 | T | G | 10.6% | 79.9 / 5.2 | 44 | E199A (GAG→GCG) ‡ | ydjH | putative sugar kinase YdjH |
| Reads supporting (aligned to +/- strand): ref base T (22/17); new base G (3/2); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-01 | |||||||||||
CCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGC > NC_000913/1856193‑1856475 | gcgGCACCCTCAGCGGCTTCTCCCCGCGCCCGATACACCAGCCGTCTGTGCCCGGCTTCATCAACACCTTTTTCCCGCCTCTCGCGAGAAAGCAGTCAGCAATAAAAACAATTATAATGATCCCGGTGAGTAATTTTGCCTCg < 2:799628/142‑1 (MQ=255)cttgCACCTTCTTCGTCATGTCACCCCGCTCGATGAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCg < 2:293249/140‑1 (MQ=255) cTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCGGTGTCTCTTTCTAGGGGAGTAATGTTGCCTGGGCATAAt > 1:567134/1‑143 (MQ=255) cTTCATCGGCCTGTCACCACGCTTGATAAAGCAGCCCTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCCCGCAAGAACGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAt < 1:675651/143‑1 (MQ=255) cGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGATTAAATTTACCTCGACAAAAtaaaaaa > 1:183064/1‑137 (MQ=255) cacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGGGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg > 1:38924/1‑143 (MQ=255) aagCTTGATGAAGCAGCCGTCTGGACCCGTTTTAAGCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa < 1:49509/141‑1 (MQ=255) ttGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAATCAGCAATTTCATCCAGTGTCTCTTCCCCGGTGAGTAATTTTGCCTCGGCCAAATTGTGGAACAAATAATCgccctg > 2:578587/1‑138 (MQ=255) gATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCGATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAAc > 1:668094/1‑143 (MQ=255) tAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCGCTTTCGCGGGGAGGAATTTGGCCTCGGCAGAATTAGGCAGGAGGTAATCGACATAACTc > 1:545477/1‑143 (MQ=255) tACCCGTTTTAATCCCCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGGGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata < 2:841504/143‑1 (MQ=255) ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAt < 2:831002/99‑1 (MQ=255) ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAt > 1:831002/1‑99 (MQ=255) tttAATCACCACCGTTTTTCCGCCGCACGCAAGAAAGCAGTCAGCAGTTTCATCCATTGTCTCTTAACAGTTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCa < 2:493257/143‑1 (MQ=255) tAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGCCATAACTCAATGCTTCGCAAAAatcatcacac > 2:618899/1‑139 (MQ=255) tCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATACCTCAATGCTTCGCAAATATCATACAGCGtt > 2:689074/1‑143 (MQ=255) tCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGtt < 1:912711/143‑1 (MQ=255) accaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCATTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACACAACCTAATGCTTCGCAAATATCATCCAGCGTttc < 2:110763/143‑1 (MQ=255) accaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTTAATGCATAGCAAAAATCAGTCAGCCttta > 2:356516/1‑142 (MQ=255) ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttca < 2:367364/143‑1 (MQ=255) ccaccGTTCTTCCGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttca < 2:853635/143‑1 (MQ=255) accGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcatt < 2:837558/143‑1 (MQ=255) accGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCGCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTAGCCAATAACCTGCAGCGTttcatt > 2:461768/1‑143 (MQ=255) ccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATACAGCGTttcattc > 1:230521/1‑143 (MQ=255) ttgttccgcACTCAAGAAAGCAGTCTGCGATTTCATCCATTTTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgc < 2:816855/138‑1 (MQ=255) acgcaAGAGAGCAGTCAGCAATTTCATCCAGCGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAGTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc < 1:823728/143‑1 (MQ=255) acgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGTAAACAGATAATCGACATAACTCAATGCTTGGCAAATATCATACAGCGTTTCCTTCAACCGAGGGTTgggt > 2:376920/1‑140 (MQ=255) gcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACCTAGCTCAATGCGTCGCAACTATGTTCCAGCGTTTCCTTCACCCGCTGCTTGATCac > 1:48048/1‑142 (MQ=255) caAGAAAGCTGTCAGCAATTTCATCCAGTGACTCGTTCCCGGCGAGTCACTTTGCCTCGGCAAAATTACGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata < 1:18932/143‑1 (MQ=255) caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCACAATTAGGACACTGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCGTTCAACAGCCGGTTggtgcta > 1:36239/1‑137 (MQ=255) gAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCg > 1:785078/1‑143 (MQ=255) aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGACACAGTTTATGTACGTTACTACATGCTTCGCAAATATCACCCCGCGTTTCATTAAACACCGGGTTGCCCATATCCCCACAGATaa > 2:13349/1‑143 (MQ=255) aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGCTTCATTCAACCGCGGTTTGATCATATCGCCACAGATaa > 2:80789/1‑143 (MQ=255) gtgtCTCTTTCCCGGTGAGTAAATTTGCCTCGGCATAGTTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATCTAGACACAGATACACATCTGCCGGGCt > 2:324040/1‑143 (MQ=255) tgtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGACACAGATAATCGACATAACTCAATGCTTCGCAAATATAATCCAACGTTTAATTCAAACACGGTTAGATAATATCGGCACAGATAATCATCTTCCgagaac > 1:411648/1‑138 (MQ=255) tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGACCATATCGGCACAGATAACCCTCTGCCGGGCTTTatc > 1:392882/1‑141 (MQ=255) gTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGTTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTaga > 2:916559/1‑141 (MQ=255) tAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAAttt < 2:785078/143‑1 (MQ=255) tAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTTCGTAACAAttt > 2:624917/1‑143 (MQ=255) aaTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTc < 1:505347/143‑1 (MQ=255) aaTTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTCAGCTTGCGTAAAAATTTc > 1:831059/1‑143 (MQ=255) aTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCt < 2:819674/143‑1 (MQ=255) ctcgcatCGGCAAAATTAGGAAACAGATAATCGACATACCTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGt < 2:567134/137‑1 (MQ=255) gCCTCGGCAAAACTAGGAAATAGATCATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAg < 2:48048/143‑1 (MQ=255) ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCACTACTTCGCAAATATAAACCAGCGATTCATTCAAACGCGGATTGATCATATAGGCACAGATAATAATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTaac > 1:593171/1‑141 (MQ=255) tCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAgcgc > 1:599411/1‑143 (MQ=255) | CCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGC > NC_000913/1856193‑1856475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |