| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | 100% | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 100.0% | 128.1 / ‑6.7 | 47 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (24/21); total (26/21) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATC > NC_000913/124105‑124370 | tggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTa > 1:280754/1‑143 (MQ=255) cACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAAGCCTTGCTCATACCAATAAAGGTAACGGCATGGGAGACGCGGCTGAAGGTAAAAACATCGCGCACCCGGTCAGGaa > 2:536188/1‑143 (MQ=255) gATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAACGCGACAGTACTCCTTGCTCATACCATTAAAGGTTACGGCATAGGCGACGCGGCAGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAt > 1:255100/1‑143 (MQ=255) gATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTACGAAAAt > 1:223046/1‑143 (MQ=255) agcggaagagAATCTACGCTGCATGCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTGGGTCATAGCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGTGCACTAGGTTAAGAAAATg < 2:855392/139‑1 (MQ=255) cgggagaaAATCGACGCTGCATACAAGAAAGGGCGGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGaa < 1:760741/139‑1 (MQ=255) gaagaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACAAAAGGCAAAGCGACAGTAATCCTTGCTCCTACCATTTAGGGTCACGGCATGGGCGACGCTGATGAAGGTAAAAACAACGCGCAATAGGATAAGAAAATGAACa > 1:123792/1‑143 (MQ=255) gaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTCATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACAAGGTTAAGAAAATGAACATgg > 1:703526/1‑143 (MQ=255) aaaaTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTGAAGAAAATGAACATGGa > 2:290707/1‑143 (MQ=255) cTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtg < 1:38794/143‑1 (MQ=255) cGCTGCATTCAAGAAAGTGCAGGGAACCTAAGGCAAAGCGACAGTAAGCCTTGCTCATGCCATTAAAGGGTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGc < 1:728366/143‑1 (MQ=255) aTTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACTGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCata > 2:549724/1‑143 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGCACATGGACGGTGTGCGTCATATCcg > 1:98253/1‑143 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg < 2:280754/143‑1 (MQ=255) aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgc < 1:315243/143‑1 (MQ=255) aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgc > 1:58898/1‑143 (MQ=255) gAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGCAGGCGAGAATGGCCCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgcg > 1:664318/1‑143 (MQ=255) gAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGCACGGTGTGCGTCATATCcgcg > 2:282325/1‑143 (MQ=255) ccAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTCAAAACAGCTCTCACTAGGTTAAGAAATTGAACATGGACGGTGTGCGTCATATCGGCGACCGTTTCAAtgtg > 1:247280/1‑143 (MQ=255) cAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGCGCGCGTCATATCCGCGACCGCTTCAATGTGc > 2:510206/1‑143 (MQ=255) taaGGCAAAGCGACAGTAATCCTTGCTCATTCCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCTTCATATCCGCGACCGTTTCAATGTGcc < 2:44582/142‑1 (MQ=255) aaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg < 1:452115/143‑1 (MQ=255) aaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg < 1:822811/143‑1 (MQ=255) ggCAAAGCGACAGTAATCCTTGCGCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGgt < 2:651932/143‑1 (MQ=255) aGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTg > 2:556575/1‑143 (MQ=255) gCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAATGATGGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGa > 1:418752/1‑143 (MQ=255) gACAGCGATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACGTCGCGCACTAGGTTAAGAAAATGACCATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATg < 2:14890/143‑1 (MQ=255) ggaaTCCTTGCTCAAACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGa < 1:761078/141‑1 (MQ=255) tCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATc < 1:892224/143‑1 (MQ=255) tCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTCCGTCATATCCGCGACCGTTTCAATGTGCCAGTGTCTGATGCAGATATc > 2:21647/1‑143 (MQ=255) tACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGc > 1:835608/1‑143 (MQ=255) tAAAGGTGACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAGGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACa < 2:658212/143‑1 (MQ=255) aGGTTACGTCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCa < 2:468456/143‑1 (MQ=255) aCGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTAATATATGATGCTTTTTCAATGTGCCGGTGTCTGATGCAGATACCGAAAAACTGCCGTACATCACCTTc > 1:665759/1‑143 (MQ=255) cGGCATGGGCGACGCGGCTGAAGGTAAAATCGTCGCGCCCTAGGTTAAGATAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTcc < 2:486348/143‑1 (MQ=255) aTGGGCGACGCTGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGa < 2:667437/143‑1 (MQ=255) ggCGACGCGGCTGAAGGTAAAAACATCGCGCGCTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACGTTCCCGGAAgg > 2:518443/1‑143 (MQ=255) cGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGtt > 2:888892/1‑143 (MQ=255) gACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACACCACCTTCCCGGCAGGTTc > 1:133463/1‑143 (MQ=255) cggcggAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGaa < 2:850251/143‑1 (MQ=255) gcggAAGGTAAAAACGTGGCTGACTAAGTTAAGAAAATGAACATGGACGGTGTGCGGCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAaga < 2:355336/140‑1 (MQ=255) tGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACGTGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAACACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGc > 1:870294/1‑143 (MQ=255) tGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACAGCACCTTCCCGGAAGGGTCTGAAGAGc > 1:480206/1‑143 (MQ=255) aaGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCAt < 1:888892/143‑1 (MQ=255) aGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATa > 1:824387/1‑143 (MQ=255) ggTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATAc < 1:637950/143‑1 (MQ=255) aaaCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGCAGGTTCTGAAGAGCATAccaaac > 1:549847/1‑139 (MQ=255) | TGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATC > NC_000913/124105‑124370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |