Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,883,027 C→G 13.1% intergenic (+30/‑161) dmlA → / → yeaV D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)/putative transporter YeaV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,883,0270CG13.1% 93.1 / 10.1 40intergenic (+30/‑161)dmlA/yeaVD‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)/putative transporter YeaV
Reads supporting (aligned to +/- strand):  ref base C (19/15);  new base G (3/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.59e-02

GCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTACGCGTACGTTA  >  NC_000913/1882890‑1883169
                                                                                                                                         |                                                                                                                                              
gcagcaATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAACTTATTTTGCGTTAAGGTCAAACCAGTTGATTTGAACCGCGTCACTGAcg                                                                                                                                           >  1:618520/1‑143 (MQ=255)
    caATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTAACTGACGCGGt                                                                                                                                       >  2:598191/1‑143 (MQ=255)
            gaagTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTCACGTTATGGTCAAACGAGTTCAGTGGAGCCGCGTCACTGACGCGGCTTCTCTAt                                                                                                                               >  2:146306/1‑143 (MQ=255)
                    tGCGCACGGGCCGAACACACCTGTTAAGAAAGGCAATGCCACCACGCCACAGGTGGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCtt                                                                                                                       <  2:199488/143‑1 (MQ=255)
                      cgcccGGGCCGAAAACACATGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCATACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTg                                                                                                                     <  1:413071/139‑1 (MQ=255)
                       tCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGATTGCCGACGCGATTTGCAAAATTATATTGCGTTAAGGTCAACCCAGTTTAATTAACACTCTTCACTTACGCGCTTTATTTATTCTTTATTTGc                                                                                                                    >  2:661463/1‑143 (MQ=255)
                                     cacCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGCTTTTTTATTCGTTCTTTGCAGTAAATAAcataa                                                                                                      >  1:351648/1‑139 (MQ=255)
                                       ccTGACATGCATGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGTACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGt                                                                                                    <  2:762698/143‑1 (MQ=255)
                                          gATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCAt                                                                                                 >  1:387255/1‑143 (MQ=255)
                                            tatGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTGGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGACAttt                                                                                               >  2:580909/1‑143 (MQ=255)
                                             atGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCCAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCCGCGTCATTTc                                                                                              >  2:665528/1‑143 (MQ=255)
                                              tGAAAGGCAATGCCACCACGCCACAGGTTGCCGAAGAGATCGCCAGAAGTATGCACCGTTACGGACAAACAAGGTTCTTCGAACCGCGTCACTGACGAGGTTGTCTCATTCGTTCTGTGCAGGAAATAACGCGCGTCagctag                                                                                             >  2:194190/1‑138 (MQ=255)
                                                aaaGGCAATGCCACCACGCCACGGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCAcc                                                                                           >  1:50991/1‑143 (MQ=255)
                                                 aaGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGTACCGCGTCACTGAAGCGGCTTTTGTATTCGTTCTTTGCAGTAAATAACCTGCGTCATGTCaacc                                                                                          >  2:737146/1‑140 (MQ=255)
                                                           caccacGCCACAGGTTGCCGACGCGATTTGCAAAATTAGTTTGCGTTAAGGGCAAACCAGTTTATTTGAACCGCGGCACGGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGttt                                                                                <  1:484110/143‑1 (MQ=255)
                                                                  ccACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTc                                                                         <  2:649411/143‑1 (MQ=255)
                                                                           gCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTAt                                                                <  1:429625/143‑1 (MQ=255)
                                                                                  cgATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTc                                                         <  2:207506/143‑1 (MQ=255)
                                                                                      ttGCTAAATTGTTTTGTGTTATGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTa                                                     <  2:430675/143‑1 (MQ=255)
                                                                                       tGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTAt                                                    <  2:572005/143‑1 (MQ=255)
                                                                                          aaaaTTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTCATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCATTTTTTATGGCTTTCCCTATTCt                                                 >  2:76721/1‑143 (MQ=255)
                                                                                              ttattTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAAt                                             <  1:692857/143‑1 (MQ=255)
                                                                                                  tttGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTCTTCTTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACTTTTTATTGTTTCCGTCTCGTGCTTTATGCGTGTCCGTCTTCTTCATTGtt                                         >  2:141549/1‑143 (MQ=255)
                                                                                                   ttGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGTCTTTCCGTATTCTTAATTGttt                                        >  2:469512/1‑143 (MQ=255)
                                                                                                    tGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTa                                       >  2:410574/1‑143 (MQ=255)
                                                                                                      cGTTAAGGTCAACCCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTTCCCTTTTATTGTTTCCGTTTCGTATTTTATGGCTTTCCGCACTCTTCATCATTTacc                                     >  2:75832/1‑141 (MQ=255)
                                                                                                       gTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCGCCTTTTCTTGTTTACGTTTAGAGATGGTCGGCTTGCAGTATTATTAATTGTTTAAtt                                    >  2:223120/1‑143 (MQ=255)
                                                                                                          aaGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAt                                 <  2:350429/143‑1 (MQ=255)
                                                                                                             gTCAAACCAGTTTATTTGAACCGTGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTCTGtt                              >  1:608981/1‑142 (MQ=255)
                                                                                                                aatgctgTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTAAGTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCGTTCCGTCTTCTGAATTGTTTGATTTATGTAACa                           <  2:114004/137‑1 (MQ=255)
                                                                                                                     ggTTTATTTGACCCGCGTCCCTGACGCGGTTTTGTGATTCGTTCTTTGGGGTAGATAATCTGCGTCATTTCATCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCaa                      <  2:146296/142‑1 (MQ=255)
                                                                                                                       ttttttGGAACCGCGTCACTGACGCGGTTTGTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAAt                    <  2:488007/143‑1 (MQ=255)
                                                                                                                           aaaaaaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGtt                                         >  2:432131/5‑118 (MQ=255)
                                                                                                                           aaaaaaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGtt                                         <  1:432131/114‑1 (MQ=255)
                                                                                                                           aaaaaaCCGAGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGtt                                         >  2:433047/5‑118 (MQ=255)
                                                                                                                           aaaaaaCCGAGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGtt                                         <  1:433047/114‑1 (MQ=255)
                                                                                                                              gAACCGCGTCACTGATGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAAGTTATGTAACATGCAAATTTTGTTa             <  2:297026/143‑1 (MQ=255)
                                                                                                                               aaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGGAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTAc            >  1:486572/1‑143 (MQ=255)
                                                                                                                                aCCGCGTCATTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAACTAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTAcg           <  2:613034/143‑1 (MQ=255)
                                                                                                                                    cgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTACGCGTa       >  1:825752/1‑143 (MQ=255)
                                                                                                                                         cTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTCTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTACGCGTACGTTa  <  2:618520/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                              
GCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTACGCGTACGTTA  >  NC_000913/1882890‑1883169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑