| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,085,955 | A→G | 10.9% | F370L (TTC→CTC) | plaP ← | putrescine:H(+) symporter PlaP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,085,955 | 0 | A | G | 10.9% | 116.6 / 8.5 | 56 | F370L (TTC→CTC) | plaP | putrescine:H(+) symporter PlaP |
| Reads supporting (aligned to +/- strand): ref base A (29/20); new base G (3/3); total (33/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-02 | |||||||||||
CTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCTTTT > NC_000913/2085814‑2086096 | cttcCAAGTTAACCCAGCTCGCACCACCGGCCAGCGCACCATCCATCTGCATGAACAGATACTGTAAGTGATCTTTCATCGTCTTGTTACGCTTCTCACGGATCCAGAACTTCGAGATGACCGACAGGTTAACGAAAGTGAAc < 2:915281/143‑1 (MQ=255) ttcCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACCGGAAGAAAAATTTCAGCGTCTTGTTACGCGTCCCACGGAACCAGAACTGCGACCGGACCGACCGGCTGACGAAACTCAACg > 2:140875/1‑143 (MQ=255) cAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGCACGcca > 1:93117/1‑143 (MQ=255) gTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCCGGAACAGATACTGGCAGTGATCTTTCAGCGTCTCGTTACGCTTCTCCCGGACCGAGCACTAGGAGATGCCGGACAGGGTAGCGACAGTGACCGccagca > 2:596161/1‑143 (MQ=255) ttAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGGCTTGTTACGCTTCTCCCGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGAGAACGgcacccg > 2:294447/1‑141 (MQ=255) cccACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCa < 1:732243/143‑1 (MQ=255) accACAGGGCACCAACGGTCAGCGCACCACACAGCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTGACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCa < 1:162552/142‑1 (MQ=255) ccACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGACGTGATCTTTCAGCGGACGGTGACGCTTCTCACGGATCCAGAACTGCGAGGTGACCGACAGGTTAGCGAGCGTGAACGCCACGAGCGCAc > 1:817904/1‑143 (MQ=255) ccACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGCGATGACCGACAGGTTAACGAAAGTGCACGCCACCAGCGCAc > 1:520395/1‑143 (MQ=255) acaGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGCCCGGGTAACGAAAGTGAACGACACCAGCGCACCa > 1:671725/1‑143 (MQ=255) acaGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCa > 1:826325/1‑143 (MQ=255) aGCGCGCCAACGGTCAGCGCAACACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCaaa < 2:299672/143‑1 (MQ=255) aGCGCACCAACGGTCAGCGCACCAGACATCGGCAGGAACAGATACTGGAAGTGATTTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGTCACCAGCGCACCaaa < 2:73772/143‑1 (MQ=255) gTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCCCCGCACCAACGTTAATCCgcgc > 1:422510/1‑143 (MQ=255) acacaTCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCCAGGTTAATCAGCGCCGCTGCCAttc > 2:870299/1‑142 (MQ=255) acacaTCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTACGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTa > 1:485267/1‑143 (MQ=255) acaTCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACCGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTAcc < 2:511885/143‑1 (MQ=255) gCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCg > 2:520310/1‑143 (MQ=255) ggAACAGACACTGGAAATGATCTGTCAGCGTCTTGTTACGCGTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAg < 1:636919/143‑1 (MQ=255) aCATATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTg < 2:133941/143‑1 (MQ=255) gATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCAATAACTGCGAGATGACCGACCGGTTCACGAAAGTGAACGCCCCCAGCGCACCAAAGTTAATCCGCGGCGTCGCCACTGCCAGGCCGAAGtcgctt > 2:894709/1‑138 (MQ=255) gAAGTGAGCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGCCAGGCTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCcagca < 1:380787/143‑1 (MQ=255) gTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAggg < 1:739630/143‑1 (MQ=255) tGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACAAGGTCGAAGTTGATTGCCAGCAGGGc > 1:84505/1‑143 (MQ=255) gaccgttCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCa < 1:399943/138‑1 (MQ=255) aTCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCCGGTCGAAGTTGATTGCCAGCAGGGCag > 2:270596/1‑142 (MQ=255) tttCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATcg > 2:803657/1‑143 (MQ=255) tttCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATcg > 1:111951/1‑143 (MQ=255) tttCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATGACCAGGTCGAAGTTGATTGCCAGCAGGGCAATcg > 2:290618/1‑143 (MQ=255) gCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAGCGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATc > 2:428834/1‑137 (MQ=255) gCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAGCGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATc > 2:4439/1‑137 (MQ=255) gCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAGCGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATc > 2:6306/1‑137 (MQ=255) gCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAGCGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATc < 1:4439/137‑1 (MQ=255) gCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAGCGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATc < 1:428834/137‑1 (MQ=255) gCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAGCGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATc < 1:6306/137‑1 (MQ=255) cGTCTGGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAACCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCaa < 2:817904/143‑1 (MQ=255) ttACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGgat > 1:520746/1‑143 (MQ=255) tgaCGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAGAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGgat < 2:520395/141‑1 (MQ=255) tACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGCCGACGTTGAGTGCCAGAAGGGCAATCGCGACAGCCAGgatg > 1:775823/1‑143 (MQ=255) ggATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGcc < 1:420127/143‑1 (MQ=255) aGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCCGGGCAATCGCGCTAACCAGGTGGATGGTCATCGCCGTAgga > 1:758456/1‑141 (MQ=255) aGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTa > 1:540311/1‑143 (MQ=255) gAACTGCCCGCTGACCGACTGGTTGTCGAAAGTGAAGGCTTCCATCGCACCAAAGTTAATCAGCGCCGTTTCCATTACCAGTTCGAAGTTTATTTTCAGTAGTGCAATCGCGCCAACCATGATTTTGTTCATCGCCGGAGTAc < 2:145047/143‑1 (MQ=255) acgACCGACGGGTTACCGAAGGTGTACGCCAGCAGCGCACCAAAGTTAATCAGCACCGTTGCCATTACCAGGTCGATTTTCATTTCCAGGCGTGCAATTGCGCCAACTAGGATGATGTTCATCGTCGGAGTACGCCATTTCgg < 2:552271/141‑1 (MQ=255) cgagtggTTTACGAAGGTGAACGGCACCAGCGCACCAAAGTTAATCAGCGCCGTGGCCATTACCAGGGCGAAGTTGATTGCCAGCAGGGCAATCGCGCTAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTa < 2:576682/138‑1 (MQ=255) cGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGCGTa > 2:888428/1‑143 (MQ=255) gACAGGTTAACGAAAGTGAACGCCACCAGCGCACAAAAGTTAATCAGCGCCGTGGCCATTACCAGGTCGAAGTTGATTGCTAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCAGGAGTACACCATAACGGGTGTAc > 1:625184/1‑143 (MQ=255) aCAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTCCGGGTGTACa > 1:479861/1‑143 (MQ=255) gTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTATACATAAc > 2:493218/1‑143 (MQ=255) ttAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAAcc > 2:235912/1‑143 (MQ=255) accGAAAGTGCACGCCACCAGCGCACCAATGTTAAGCAGCGCCGTTGCCATTGCCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCga < 2:827491/141‑1 (MQ=255) gAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCCATCGCGCCCACCAGGATGATGTTCATAGCCGGAGAAAGCAAATGCGAGTGTACATAACCgaaga > 1:232752/1‑143 (MQ=255) gAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGTAGTACGCCATTTCGGTTGTACATAACCgaaga > 1:904943/1‑143 (MQ=255) aaaGTGAACGCCACCAGCGCACCAAAGTTAAGCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCgaagaa < 1:635685/143‑1 (MQ=255) aaGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCGACTTCGGGTGTACACAACCGacgaag > 2:539054/1‑143 (MQ=255) gTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATGACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCt < 1:460996/143‑1 (MQ=255) aaCGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCtttt > 1:774144/1‑143 (MQ=255) | CTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCTTTT > NC_000913/2085814‑2086096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |