| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,090,124 | C→G | 7.0% | intergenic (+78/‑68) | hisL → / → hisG | his operon leader peptide/ATP phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,090,124 | 0 | C | G | 7.0% | 155.6 / 4.6 | 57 | intergenic (+78/‑68) | hisL/hisG | his operon leader peptide/ATP phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (30/23); new base G (2/2); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01 | |||||||||||
CACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTG > NC_000913/2089983‑2090266 | cacaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa < 1:821925/143‑1 (MQ=255) acaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc < 2:329733/143‑1 (MQ=255) ttttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg > 2:396929/1‑143 (MQ=255) tttcATGACACGCGTTCAATTGAACCCCCACCATCAGCACCATCTTCCTGACTAGACTGCCAGGCGATGTGTGCTGGAAGACCTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggg < 2:735940/143‑1 (MQ=255) tttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg < 1:617027/143‑1 (MQ=255) ttgaTGACGCGCGTTCAGTTTACACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATGCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg < 2:900553/140‑1 (MQ=255) tATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAATACATTCAGATCTTCCAGTGGTGCATGGACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGc > 2:109306/1‑143 (MQ=255) gACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGGATGAGAAAGCCACCGGAAGATCACCTTCCGGGGGCtgt > 2:697205/1‑141 (MQ=255) acacGCGTTCCATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtttt < 2:60509/143‑1 (MQ=255) acGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGACAGCCCCCGGACGATCACCTTCCGGGGGCcttttt > 2:327457/1‑143 (MQ=255) aaTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCCGATCTTCCAGTGGTCCATCCACACATGAGAAAGCCCCCGGAAGATCCCCTTCCGCGGGCTTTTGTCTTGcgcg > 1:159749/1‑143 (MQ=255) aaCACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTCGTGCATGAACGCATGAGGATGTCCCCGTAAGATCACCTTCCCGGGGCTTCTTTATTGCCCGgatgat > 2:400239/1‑143 (MQ=255) acacCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGGTGATa > 2:838560/1‑143 (MQ=255) acaccatcatcaccatcatCCTGTCTAGTATTTCAGGCGATGTGTGCTGGAAGACATTCAGAACGAGAAGTTGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACg < 2:493936/142‑1 (MQ=255) tcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa > 2:83900/1‑143 (MQ=255) atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTGTaaa > 2:867844/1‑143 (MQ=255) tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag > 2:13139/1‑143 (MQ=255) ccTGACTAGTCTTTCAGGCGATGTGTGCTGCAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCGCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga < 2:53396/143‑1 (MQ=255) cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAagag < 1:475358/143‑1 (MQ=255) aCTAGTCTTGCAGGCGATGGGTGCTGGAAGACATTCAGAGCTTCCAGTGGTGCATGAACGCATGAGAAAGCTCCCGGGAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa < 1:406243/143‑1 (MQ=255) tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGTAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGATTAAAGAGGCATAACaaa > 2:362417/1‑143 (MQ=255) tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTAAGACAGGTTGAAAGAGGAATAACaaa > 2:775489/1‑143 (MQ=255) tCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAAt < 2:219321/143‑1 (MQ=255) gggATGTGTGCTGGAAGACATTGAGAGCTTCCAGTGGTGCACGGCAAACTGATAGATGCGCGGGGTGATCACCATCCGGGGGCGTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca < 2:889772/141‑1 (MQ=255) gCGATTTGTGCGGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca < 2:781032/143‑1 (MQ=255) gATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTATAAAGAGGAATAACAAAATgacaga < 2:553766/143‑1 (MQ=255) tgggtgCGGGACGACACTCAGACCTTCCAGTGGCGCATGACCGCAGGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagaca < 2:568483/140‑1 (MQ=255) gtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaa < 1:396929/143‑1 (MQ=255) tgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCCCCTTCCGGGCGCTTTTTAAATGCGCGGATGATAACGGAACAGAAAAGAAAAAAGAGAAATAACAAAATGACAGacaac > 1:104725/1‑143 (MQ=255) tgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTTAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaac < 2:16544/143‑1 (MQ=255) gtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGccaacc > 1:891315/1‑142 (MQ=255) gtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaaca < 1:719626/143‑1 (MQ=255) tgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAGCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGCCAGGTTTAAAGAGGAATAACAACATGACAGACGacac > 2:515974/1‑143 (MQ=255) aTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCa > 1:508551/1‑143 (MQ=255) ttCAGATCTTCCAGTTGTGCATGAACGCATGACAACACCCACTTTATATCGTCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTGCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCAt < 2:642810/143‑1 (MQ=255) ttCAGATCTTCCAGTGGTGGATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCAt < 1:32871/143‑1 (MQ=255) tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAACGGGGGCTAGCAAGACGCCCGACACCACTCGTTTACGCATa < 2:408590/143‑1 (MQ=255) tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATa < 2:229621/143‑1 (MQ=255) gATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGGTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCt > 2:550208/1‑143 (MQ=255) aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACGAAATGACAGACAACACTCGTTTCCGCATAGCTa > 2:788784/1‑143 (MQ=255) ttCCAGTGGTGCATGAACGCATGGGAAAGCCCCCGGAAGATCACCGTCCGGGGGCTTTTGTATTGCGCGGTGGATAACGGTTCGGAGAGGTTTAAAGAGGAATAACAAAGTGACAGACAACACCCGGTTACGCAGAGCTATGc > 1:236601/1‑143 (MQ=255) ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGc > 2:206002/1‑143 (MQ=255) acAGTGGTGCATGAACGCATGAGAAAGACCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAg < 1:440550/142‑1 (MQ=255) gtgCATGAACGTATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATcc < 1:83900/143‑1 (MQ=255) gCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAACTGTCAGACAACACTCGTTTACGCATAGATATGCAGAAATACGGCCGGTTaa > 1:134334/1‑143 (MQ=255) gCATGAGAAAGCCCCCGGAAGATCACCTGCCGGATGCAGGTTTATTGCCCGGTTGAGAACGGCTCAGAGCGGACTGAATAGGAGAAACAAGATGACAGCCAACACTCGTTTACGCATACCAAGGCAGAAATGCGCCCGcttca > 2:848021/1‑141 (MQ=255) gCATGAGAAAGCCCCCGGAAGATCACATTCCGGGGGCATTTTTATTGCACGGTTGATAACCGTTCAGACAGCTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACACATACCTATCCAACAATCCGGACGTTTaa > 2:847243/1‑143 (MQ=255) aaaaaGCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGGGTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATACAGCCGATTAAGtgat > 2:731718/3‑143 (MQ=38) agtctccGTAATATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACt < 1:682403/138‑1 (MQ=255) cccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAACGAGGAATAAGAAAATGACGAACACAACTAGTTTACGCATGGCTATGCAGAACGCCGCCCGATTAAGcgatgaaacc > 2:93589/1‑139 (MQ=255) ccccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTCAAGAGGAATAACAAAATGACCGACAACACTCGTTTACGCATAGCTATGCAGAAATACGGCCGTTTAAGTGATGACTctc > 1:125937/1‑141 (MQ=255) ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCAc > 1:647015/1‑143 (MQ=255) cccGGAAGATCACCTTCCGGGGGCTTTTTTTTTTTTTTTTTGATAACGGATCAGACAAGCATAAAGAGGCATTACAATAGGACAGAAAAAAGTGGTTTACGCATGTCTATGCCGAAATCCGCCCGTTTAAGTGATGCCTCAcg > 2:383433/1‑143 (MQ=255) cccGGAAGATCACCTTCCGGGGGCTTTTTTCTTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATACCAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGCAATCCGGCCGTTTAAGTGGTGGCTCAcg > 1:110135/1‑143 (MQ=255) ccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTCTGCAGAAGTCCGGCCGTTTAAGTGATGACTCCcgc > 1:797028/1‑143 (MQ=255) aTCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAAGAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCCCGCGAATTGc > 1:712516/1‑143 (MQ=255) tCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCt > 2:309313/1‑143 (MQ=255) cACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGCATGGCTg > 1:310914/1‑143 (MQ=255) | CACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTG > NC_000913/2089983‑2090266 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |