| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 100.0% | 108.7 / NA | 37 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (16/21); total (16/21) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132647‑2132925 | gggtttGGTCGAGGGTAAAGAGCGTCTATTTTTGGCGGGCGCGGTCGAATTTGTCTTTGGGTTGGCGGTAAAAGTGGAAGTGTCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc < 2:379927/138‑1 (MQ=255) aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATACAGGCTCTg > 2:421444/1‑143 (MQ=255) tGGTAGAGGGGAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt < 2:11731/143‑1 (MQ=255) gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCct > 1:307452/1‑143 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCgctt > 1:116771/1‑143 (MQ=255) ttgaaCATCTGAGATTTTTGGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc < 2:729366/140‑1 (MQ=255) cgCTGATAGTGTTTGCTGGCGCGGTCGAATGTGTCTTTGTGCTTGCGGTAAAAGTGGAAGGACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt < 2:36652/142‑1 (MQ=255) tcgtgtatTTTTTTCTTGCGGTGTCGAATCTGTCTTTCTGCTTGCGGTAGAAGTGTAAGGACTCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGTCTCTGCGTCGCCTCTTCTACTTTc < 2:524980/138‑1 (MQ=255) tGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:357031/1‑143 (MQ=255) gtGGCGCGGTCGAATTTGTGTGTGTGCTTGCGGGAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGGGGGGATCTGCATTTCACCGTGATTGAGATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa < 2:276415/142‑1 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 2:905103/1‑143 (MQ=255) ggagtttGCCTTGGTGCGTGCGGTAAGGGTGGAAGTGCCCGGAGAATTTTTTCGGGGACGAGGTGATCTGCATTTCACCGTGATAGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc < 2:452760/139‑1 (MQ=255) cGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTATGAGTAGCCACTTCTTCTTTCCACGGTTCGCCGTACTccaccacc < 1:870229/143‑1 (MQ=255) tCTTTGTGCTTGCGGTAAAAGTGGAAGTATCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg < 1:756582/143‑1 (MQ=255) tAAACGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc < 1:156446/143‑1 (MQ=255) tgtgtatgTACCCTGAGAATTTTTTTTGTTACGTTGTGTTTTTTATTTCACCTTGATTGATATGCAGGCTCTTCGTCGTCTCTTCTACTTTCCTTGGTTCGCCGTTTTTTATCTTCATCCGCAGGAAGATGTCGTAATCCtgt < 2:497955/136‑1 (MQ=255) aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACGGTGATTGATATGCAGGCTCTGGGTCGCCTCATCTACTTTCGAGGGTTCGCCGGACTCCACCACCATCCGCAGGGAGAGGGCGTGAtgatgt > 2:302009/1‑138 (MQ=255) cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa > 1:487842/1‑143 (MQ=255) cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCCGGCAGATATCGTAACCCTCTCCCGcctcccgg > 2:210550/1‑136 (MQ=255) cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATACAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAgg > 1:10210/1‑142 (MQ=255) gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc < 1:896062/143‑1 (MQ=255) tgaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:48450/142‑1 (MQ=255) agaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATCGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCTGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:690114/143‑1 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCCTAAATCAGTACCGCTAGCAGCTCGGTCTCg > 1:160855/1‑143 (MQ=255) tttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGa < 1:905103/143‑1 (MQ=255) gTGCCGCGGTGATCTGCTGTTCGCCGTGATTGATCTGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 2:209529/143‑1 (MQ=255) gTGACGAGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGATACCGaactga < 2:507560/143‑4 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACAACCACTCTTTGag > 1:301981/1‑142 (MQ=255) cATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGACGCTGTCGTCCTCCTCTGCCGCTTTCAGTTCGGTATAGAACAAACACTGTGTGACAAGcc > 1:656513/1‑143 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCCCCGTACTCCACCACCCTCCGCAGCAAGATGTCATAAAAATATACCACTTTCAATTCGGTATCCAACAAACACGCATTGAAACGCCCTGACCac > 1:867232/1‑142 (MQ=255) ttattgatATGCAGGCTCTGCTTCGTCTCTTCTACTTTCTACGGTTCGCCGTAGTCCACCACCATCTGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCTAACAAACACTCTTTGAAACGCCATGCCCAGGt < 2:509119/141‑1 (MQ=255) ttgatATGCAGGCTCTGCTTTTCCTCTTCTACTTTCGCCGGTTCGCCGTACTCCACGACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa < 2:13904/143‑1 (MQ=255) tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTCCGGTATCGAACCAACACTCTTTGAAGCGCCATGCCCAGGTCAAg > 1:709799/1‑143 (MQ=255) atagGCAGGCTCTGCGTCGCCTCCTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc < 1:125228/143‑1 (MQ=255) atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt < 2:116771/143‑1 (MQ=255) atGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCGTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACGAACGCACTTTGAAACGCCATGCCCAGGTAGAGAccc > 1:38735/1‑142 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAGCGCACAAACACTCTTTGAACCGCCATGCCCAGGTAAAGACCTGGt > 2:643587/1‑143 (MQ=255) | GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132647‑2132925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |