Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,821 C→G 100% C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,8210CG100.0% 119.7 / NA 40C104S (TGC→TCC) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (16/24);  total (16/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTA  >  NC_000913/2661682‑2661959
                                                                                                                                           |                                                                                                                                          
ggTGCGGGGCGCGTCGTGAGTAGCCTGACGACCAGACACATCCGGCACTTCCTGGTTATTAACCCGTTCGCCTTGGTTAATGTTCTTGAGAAGGCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGAtt                                                                                                                                         <  2:320855/143‑1 (MQ=255)
  tGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTa                                                                                                                                       >  2:388615/1‑143 (MQ=255)
   gCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCCCAGCGCGTGGGTCAGGGATTTAt                                                                                                                                      >  1:549589/1‑143 (MQ=255)
    cGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATc                                                                                                                                     <  2:27680/143‑1 (MQ=255)
     gTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcg                                                                                                                                    <  1:642833/143‑1 (MQ=255)
      gggCGCGTCGTGAGGATGCTGACGGCCAGACACATCCAGCACTTCCTGGTTATTAACCAGTGCGCCTAAAGGAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcgc                                                                                                                                   <  2:154563/142‑1 (MQ=255)
            gtcgtGTGTGTGCTGGCGGCCAGCTACATCCAGCACTTCCTGGTTATGAACCAGTTCGCCTATAGGAATGTTGTTGAGAAAGCTGGTGAGACGTTCTCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCt                                                                                                                             <  2:844905/143‑1 (MQ=255)
                tGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGcc                                                                                                                         >  1:723368/1‑142 (MQ=255)
                         tGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAACTCACGCCACAGCGCGTGGGTCAGGGATTTCTCGCCGCCCTGCCAGCCGCCtct                                                                                                                >  2:220624/1‑141 (MQ=255)
                                  gACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTggcc                                                                                                       >  2:877488/1‑140 (MQ=255)
                                              gtttCGTGGTTATTCGCGAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATc                                                                                           <  2:910900/141‑1 (MQ=255)
                                              aCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATc                                                                                           <  1:388615/143‑1 (MQ=255)
                                                 tCCTGGTTATTAACCAGTTCGCTTAAAGTAATGTTGTTGAGAAAACCGGTGAGTCGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTAc                                                                                        <  2:69793/143‑1 (MQ=255)
                                                      gTTATTAACCGGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGAtt                                                                                   <  1:184270/143‑1 (MQ=255)
                                                      gTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGAtt                                                                                   >  1:214741/1‑143 (MQ=255)
                                                       ctattaACCAGTTCGCGTAAGTTAATGTTGTTGAGAAAACCGATGAGAAGTTCGCTTGAATCACGCCACAGCGCGGGGGTCAGGGATTTATCGCCGCCCTGGCAGTCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTc                                                                                  <  2:190058/142‑1 (MQ=255)
                                                         attaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGt                                                                                <  1:698813/143‑1 (MQ=255)
                                                         attaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGt                                                                                <  2:128553/143‑1 (MQ=255)
                                                           taACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTAGTCa                                                                              >  1:788407/1‑143 (MQ=255)
                                                                     gCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTaa                                                                    >  2:14514/1‑143 (MQ=255)
                                                                      ccTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGCTCCGTCACCGGCGCTCAt                                                                   >  1:238349/1‑143 (MQ=255)
                                                                           aGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAGCGTGACGTATTTACAGATTCGTCAACGGCCCTAATCACtt                                                              >  2:641334/1‑143 (MQ=255)
                                                                                  ttgttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAAc                                                       >  1:131440/1‑143 (MQ=255)
                                                                                  ttgttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGATGGCATCTACAGCTTCGCCAACGGCGCTAATTACGTCGCCAAc                                                       >  2:544181/1‑143 (MQ=255)
                                                                                  ttgttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACACTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAAc                                                       >  2:57820/1‑143 (MQ=255)
                                                                                   tgcggagagaGCCGGTGAGACGGGCGCGCAAAGCACGGCACAGCGCGTGGGTCAGGGATTTATCGCGACCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACg                                                      <  1:241501/139‑1 (MQ=255)
                                                                                      tgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTCCAGATTCGTCAACGGCGCTCATTACTACGCCAACGGCg                                                   >  2:110356/1‑143 (MQ=255)
                                                                                       gagaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCgc                                                  >  1:852397/1‑142 (MQ=255)
                                                                                               cGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGctgctg                                          <  2:803135/143‑1 (MQ=255)
                                                                                                 gTGAGACGGTCGCTCAAATCACGCCACGGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTCCCCTGCCAACTGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGc                                        <  2:225975/143‑1 (MQ=255)
                                                                                                         gTCGCTCAAATCACGCCACAGCGCGTGGGTCCCGGATTTATCGCCGCCCTGGACGCCGCCTTTCGCCTGAAAACGGGTGGCATCTACAGATTCGTCAACGGAGCTATTTTCTTCGCCAACGGCGATGCTGCTGGCATCTTTGc                                <  2:848237/143‑1 (MQ=255)
                                                                                                                       gCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGACATCTACAGATTCATCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAacca                  >  1:791409/1‑143 (MQ=255)
                                                                                                                        cggcggcgcGTGGGTCGGGGACTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTCCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAaccac                 <  2:657104/138‑1 (MQ=255)
                                                                                                                        ccACAGCGCGTGGGTCAGGGATTGATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAaccac                 <  1:609619/143‑1 (MQ=255)
                                                                                                                        ccACAGCGCGTGCGTCAGGGATTTATCGCCGCCCCGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAaccac                 <  1:66375/143‑1 (MQ=255)
                                                                                                                            tgtgtgTGGTTCATGGATTTATCGCCGCCCTTGCAGCTGCCTTTACTCTGATAACAGTTGGCATCTACACATTCGTCAACGGCGCTAATTACTTCGCCAACTGCTATTCTGTTTGCATCTTTGCCTAACAGATAACCACCGcc             <  2:101606/138‑1 (MQ=255)
                                                                                                                            aGCGCGTGGGTCAGGGATATATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGcc             <  1:133803/143‑1 (MQ=255)
                                                                                                                               gcgGGGGTCAGCGATGGATCGCCGCGCTGGCAGCCGCCTTTACCCTGACAACGGGGGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTgg          <  2:109402/143‑1 (MQ=255)
                                                                                                                                       cAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTa  <  2:258810/143‑1 (MQ=255)
                                                                                                                                       cAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTa  <  2:596356/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTA  >  NC_000913/2661682‑2661959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑