| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,634 | A→C | 100% | T108P (ACC→CCC) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,634 | 0 | A | C | 100.0% | 148.9 / ‑6.7 | 56 | T108P (ACC→CCC) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (20/35); total (21/35) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG > NC_000913/3041496‑3041770 | cATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCAt < 2:267798/143‑1 (MQ=255) aCGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGGGCGTGAACAGCAGCTGACTGAACTGAAGAAATATGCGGTATCCTGTAAAGTGCCGCTCGCGGCa > 2:785361/1‑143 (MQ=255) aaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacgac < 1:226232/143‑1 (MQ=255) tAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATGGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTAGTCTCTAAAGGGCCCATCGCGCCAGACGACGAGc > 2:916060/1‑143 (MQ=255) aTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAGAAATATGCGGTATTCTCTAACGTGCCCATCGCGCCAGACGACGAGCgtgt > 2:129364/1‑143 (MQ=255) tgtgtggCAATTTGCGTCTGTTCCGCGACGGCGATGGCTTTGCATGGAGTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGtgtg < 2:57585/137‑1 (MQ=255) tgtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGtgtg < 1:838204/143‑1 (MQ=255) ttAGGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAGCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtgt < 2:318556/143‑1 (MQ=255) gtACGTCTGGTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtgt < 2:391733/142‑1 (MQ=255) tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGGACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGAGCCAGACGACGAGCGTGTGCTGCTTGGTGTTCCCg > 1:835370/1‑143 (MQ=255) tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAAACGCAGATGACGGAAATGACAAGATAATTGCGATTCTCTCAAGTGCCCATCGCGACAGACGACGACCGTGGGCTGCATGGTGTTGCCg > 2:792980/1‑143 (MQ=255) tCTGTCCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCATGTGCTGCTTGGTGTTGCCg < 1:17847/143‑1 (MQ=255) tCTGGTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg < 1:410720/143‑1 (MQ=255) tGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt > 1:367674/1‑143 (MQ=255) tGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTACAAAGACACAATCGCGCGAGCCGACGAGCGTGCGCTGCTTGGTGTTGCCGGt > 2:400523/1‑143 (MQ=255) gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg < 2:136759/143‑1 (MQ=255) aCGGCGATGGCGTTGCATGGATTGAAGGGCGGAGCGGGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcg < 1:37597/143‑1 (MQ=255) gggttggCATGGGTTGTAGGGCGCGGCGCGCGTGGACCGCAGTTGACTGAACCGAAAAAAGATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGGGTGCTGCTTGGTGTTGCTGGTTTTCAGGCGcgcgccgc < 2:454393/137‑1 (MQ=255) ttGCATGGACTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg < 1:358891/143‑1 (MQ=255) tGCGTGGATTGACTGGCGCCTCGCGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg < 2:537927/143‑1 (MQ=255) tGCATGGATTGGACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg < 1:627637/143‑1 (MQ=255) aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGATTAGTGGTGACGGTTTTCAGGCGCGCGCCGCGCTGGcca > 1:861949/1‑141 (MQ=255) ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATAGGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt < 1:798462/143‑1 (MQ=255) tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct > 1:148188/1‑143 (MQ=255) ggAACCGCGCCGCGTGCGTGAACCGCAGCTGACCTGACTGACAAAGTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct < 2:658760/142‑1 (MQ=255) cgccgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg < 1:213132/140‑1 (MQ=255) cGGCCCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGGGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg < 2:856677/143‑1 (MQ=255) gcgcAGCGTTCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAACGCCCTTCAGCg > 2:370494/1‑143 (MQ=255) gcgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACCGCGCCCATCGCGCCAGACGACGAGCGCGTGCTGCTTGGTGTTGCCGGCTCTCAGGCGCGCGCCGCGCCGGCCAACCCCTTTAGCg > 1:179399/1‑143 (MQ=255) gcggcgtgcgtGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa < 1:365195/140‑1 (MQ=255) gcAGCGTGCGTGAACTGCAGCTGACTGAATTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa < 1:176463/143‑1 (MQ=255) gtggtgcgtcCACCGCAGCTGACTGAACTGCAAAAATATGCGGTATGTTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACt < 2:644734/140‑1 (MQ=255) gcgtgAACCGCAGCTGACTGAACTGAAACAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAAATGACGAGCGTGTTCTGCTTGGTGTTGCCGGTTTCCAGGCGCGCGCCGTGCTGGCAAATCTCTTTAGCGAACTGCCtt < 2:272563/143‑1 (MQ=255) gcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATGCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt < 1:196070/143‑1 (MQ=255) gtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGCGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCCTCAGCGAACTGCCTTCg > 2:60773/1‑143 (MQ=255) tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTTCCGGTTTTCAGGCGCGCGCCGAGCTGGCCAGTCTCTTTACCGCCCTGCCCTCgc > 1:390082/1‑142 (MQ=255) aagcGCAGCTGAGTGAACTGAAAAAAGATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGGGGGCGAGCGTGTGCTGCTTGGTGTTGCCGGTCTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa < 2:9571/140‑1 (MQ=255) cGCAGCGGACGGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAACCAAAAAGCTTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa < 1:568772/143‑1 (MQ=255) aGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaaa < 1:688744/143‑1 (MQ=255) gactgaACTGAATAATTATGCGGTTTTCTCTTAAGTGCCCTTCGTGTCTGACGACGAGCGTGTGCTGCTTGGGGGGGCCGGTTTTCAGGCGCGGGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg < 1:312429/143‑1 (MQ=255) tgagAAAATATGCGGTATTCTCTAAAGTTCCCATCTCGGCAGACGACGAGCGTGTGCTGCTTTGTGTTGCGGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGATCTGCCTTCGAAAGAAAAACAGGTAGTCaaa < 2:278402/143‑1 (MQ=255) tgagAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa < 2:318291/143‑1 (MQ=255) tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGGAAGAAAACCCGGTAGGCaaa > 1:606595/1‑143 (MQ=255) aaaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaaga > 2:713379/1‑143 (MQ=255) aaaTATGCGGTATTCTATAAAGTGCCCATCGCGCCAGAAGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATGTCTTTAGCGAACTGCCTTGGAAAGCACAACAGGTAGTCAGAGAggg > 1:777940/1‑143 (MQ=255) aaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgc > 1:551383/1‑143 (MQ=255) tGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa < 2:665791/143‑1 (MQ=255) cGGTGTTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGGTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACCGCCGTCGAAAGCAAAACAGGCAGGCAAGCGAGGCGCGccc > 2:450556/1‑143 (MQ=255) cGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGAcc > 1:787593/1‑143 (MQ=255) tATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACt < 1:713379/143‑1 (MQ=255) ctctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGc < 1:751476/143‑1 (MQ=255) ctctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACACGTCGTCACAGAAGACGCGACCACgttcc > 1:273029/1‑141 (MQ=255) tacaGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTAt < 2:380396/140‑1 (MQ=255) tAAAGTGCCCAGCGCGCCAGACGACGTGCGGGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTAt < 2:433009/143‑1 (MQ=255) aaaGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACCTTGCTATg > 1:533405/1‑143 (MQ=255) aaaGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGAGGCCGGGTTTCTGGCGCGCTGCGCGCTGGCAAATCTCTTTAGCGAATTGCCTTCGAATGATAAGTAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATg < 1:336495/143‑1 (MQ=255) | CATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG > NC_000913/3041496‑3041770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |