| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,512,306 | G→C | 6.3% | I13M (ATC→ATG) | yhfZ ← | putative DNA‑binding transcriptional regulator YhfZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,512,306 | 0 | G | C | 6.3% | 162.0 / 2.5 | 60 | I13M (ATC→ATG) | yhfZ | putative DNA‑binding transcriptional regulator YhfZ |
| Reads supporting (aligned to +/- strand): ref base G (30/24); new base C (2/2); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
TCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTT > NC_000913/3512164‑3512441 | tCCGTATCGCTCCGCTTGATTCCGGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGtg < 2:413167/143‑1 (MQ=255) tCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgacg < 1:283227/143‑1 (MQ=255) gCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgacgac < 1:867242/143‑1 (MQ=255) tCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGc < 2:120522/143‑1 (MQ=255) cGCTTGATTCCTGCGGTTTCAACGCGGCCTGCGTCAGGCCAACGTATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTGCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTCATCACGACGctg < 2:97874/143‑3 (MQ=255) gCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGACCGGCATTCATTTGCCAGCTCAACTATTTACTTCTATCGATTACCGCACTTTTCACCCACCAAATAACGGGCCAGCGTCGTGATGACGACGCctt > 1:209503/1‑143 (MQ=255) tGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCctt‑‑ct‑t > 2:167652/1‑143 (MQ=255) aTTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑Ct‑ttt < 1:317713/143‑1 (MQ=255) tgccAGCGTTTTGAGCGCTGCCTGTGGCAGGCCACCGGATGAACGGCGTGCATCTGCCAGCTCATCTATGGTTTTCAATCGAGTACCGCACTTTTCACCCAACAAATAACGGACCAGCGTCGTGATGACGACGCCTT‑‑Ct‑tttt < 2:683907/141‑1 (MQ=255) tCCAGCGTTTTCAACGCGGCCTGCGTCGGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑Ct‑ttttt < 1:122082/143‑1 (MQ=255) tCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCAGGTGTCGTGATGACGACGCCTT‑‑Ct‑ttttt < 2:799608/143‑1 (MQ=255) ccAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATCAACGGCATTCAGTTGCCAGCTCATCTATGGGTTTCCATCGATTACCGCACTTTTCCCCCAACAACTAACGGGCCAGCGTCGTGATGAGCACCCCTT‑‑CT‑TCTTTg > 2:389729/1‑143 (MQ=255) cAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCCGCGTCGTGCTGACGACGCCTT‑‑CT‑TTTTTGa > 1:13248/1‑143 (MQ=255) cAGCGTTTGCAACGCGGGCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCCCTTTTCACCCAACAAAGAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGa < 1:549321/143‑1 (MQ=255) aGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCCCCCAACAAATAACGGGCCACCGTCGTGATCACGACGCCTT‑‑CT‑TTTCTgct > 1:71882/1‑141 (MQ=255) aGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCGAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CTGTTTTTGa > 1:71833/1‑143 (MQ=255) gCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATa > 1:63649/1‑143 (MQ=255) ttttCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAAc > 1:402390/1‑143 (MQ=255) cAGTCCAACGGATGAACTGCATTCATTTGCCAGCTCATCTATGGTTCTCAATCGATTACCGCACTTTTCACCCAACACATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAAtt < 1:299533/143‑1 (MQ=255) cAGGCCAACGGTTGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCTACAAATAACGGGCCAGCGTCGTGATGACGATGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAAtt < 2:349602/143‑1 (MQ=255) aGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCAACCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAAttt > 2:107535/1‑143 (MQ=255) gcccAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCATCAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAAtttt < 2:79811/141‑1 (MQ=255) gCCACCGGATGAACGGGCTTCATTTGCCAGCTCATCTATGGTTTTCAATCCATTACCGCTCTTTTCACCCACCAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTc < 2:871670/143‑1 (MQ=255) ccAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCa < 1:736753/143‑1 (MQ=255) cAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGAGGGCGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGTCGCATAATAGATGTTCAg > 2:201796/1‑143 (MQ=255) aaCGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGCCGCATAATAAATTTTCAGt > 2:39349/1‑143 (MQ=255) aTGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAtt < 1:14879/143‑1 (MQ=255) cGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCGTT‑‑CT‑TTTTTGATAAACGGTCGACGCAGAATAAATTTTCAGTAAATTGaaga > 1:274700/1‑141 (MQ=255) ggCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAatat > 2:192866/1‑143 (MQ=255) gCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATGGAGGGTATTCTGCAT‑AACAC‑ACATTTTCGCT‑TCTTTTATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAtt < 1:668821/143‑1 (MQ=255) attcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAtttt > 1:897632/1‑142 (MQ=255) ttcattTGCCAGCTCATCTTGGGTTGTCAATCGATTACCGCACTTTTCACCCAACAAATAACAGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtat < 1:455516/143‑1 (MQ=255) tctattTGCCAGCTCATCTATGGTTTTCGATCGTTTACCGCACATTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtat < 2:885008/140‑1 (MQ=255) attTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAACTAACGGGCCAGCGTCGTGATGACGCCGCCTT‑‑CT‑TTTTTGATAAACGTTCGACACATAATAAATTTCCAGTACATTGAATATTTATATc > 2:409555/1‑143 (MQ=255) attTGCCAGCTCATCTATGGTTATCAATCGATTACCGCACTTTTCAACCAACAAATAACGGGCCAGCGTTGTGCTGACGACGCCTT‑‑CT‑TTTGCGATAAACGTTCGACGCATCATAACTTGTCAGTACAAGGAATACTtatacc > 2:615037/1‑141 (MQ=255) tttGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtataaga > 1:885008/1‑140 (MQ=255) gCCAGCTCCTCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTATACGCAGAATAAATTTTCAGTAAATTGAATATTTATATCTTCa < 2:894271/143‑1 (MQ=255) gCCAGCTCACCTAGGGTTGTCAATCGAGTACCGCACTTTTCACCCAACAAAGAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCa < 1:58411/143‑1 (MQ=255) tCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCACCAAATAACGGGCCAGCGTCGTGATCACGCCGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATCATACATTTTCAGTAAATTGAATACTTATATCCTCAGGacta > 2:845869/1‑140 (MQ=255) ttttCACTCGATTACCGCACTGTTCACCCAACAAATAACGGGTTGAGATAGTCAGTACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAg < 2:402390/143‑1 (MQ=255) ttCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGgc > 1:422552/1‑143 (MQ=255) ttCAATCGATTACCGCAACTCGCACCCAGCAGGTGGCGGGCTAACGACAGGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGgc < 1:904038/143‑1 (MQ=255) aaTCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCa > 1:130958/1‑143 (MQ=255) aTCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATCAACGTTCGACGCATGATAAATTTTCAGTAAATTGAATATTTATATCTTCACGAATTTGAAGATAAGGCGCaa > 2:489477/1‑143 (MQ=255) aTCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCaa < 2:63649/143‑1 (MQ=255) tCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATCACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATACATTTTCAGTAACTTGAATCTTTATATCTTCAGGAATTTGAAGATCAGGTACAAc > 2:480117/1‑143 (MQ=255) gATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAg < 1:477061/143‑1 (MQ=255) cGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGGATATTTATATCTTCAGGGATATAAACATATTGCGCAATCGCgagct > 1:26016/1‑141 (MQ=255) ttttCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATGTTTATATCTTCAGGAATTTGAAGATAAGGCGCGACAGCGAGGTGTGGa > 1:481093/1‑143 (MQ=255) aCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACgg > 2:757388/1‑143 (MQ=255) aCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGCCTCAACAGCGAGATGTGGAACCgg > 2:143257/1‑143 (MQ=255) cccAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGc > 1:357418/1‑143 (MQ=255) caacaaATAACGGGGCAGCGTCGTGATGACGTCGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGa < 2:616166/143‑1 (MQ=255) caacaaATAACGGGCCTGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGa > 2:332000/1‑143 (MQ=255) caacaaATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGa < 1:536668/143‑1 (MQ=255) tAACGGGGCAGCGGCGTGATGACTACGCCTT‑‑CT‑GTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACtt < 2:872569/143‑1 (MQ=255) ccAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTATATTGAATATGTATGTCTTCAGTAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGGGAGGCACTTCACAttt > 2:546278/1‑143 (MQ=255) ggggtcgtTATGGCGACGTCTG‑‑CT‑TTTTTGATAAACGTTCGACGGATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACAttttt < 2:507109/140‑1 (MQ=255) ggCGTCGTCATCACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGCGATGTGGAAACGGCGAGGCACTTCACAttttt > 1:848345/2‑143 (MQ=255) gCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACAtctttt > 2:581540/1‑143 (MQ=255) gCGTCGTCATCACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTTAATATTTATATCTTCAGGCATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACAtttttt > 1:782110/1‑143 (MQ=255) | TCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTT‑‑CT‑TTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTT > NC_000913/3512164‑3512441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |