| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,601,825 | G→C | 11.2% | P88A (CCC→GCC) | ftsX ← | cell division protein FtsX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,601,825 | 0 | G | C | 11.2% | 106.1 / 4.4 | 43 | P88A (CCC→GCC) | ftsX | cell division protein FtsX |
| Reads supporting (aligned to +/- strand): ref base G (14/24); new base C (3/2); total (17/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
TCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAG > NC_000913/3601686‑3601967 | tCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAg > 2:151812/1‑143 (MQ=255) gtccTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGGCTTTGTACACCATATCACCGACGCTGTGCAGCg > 2:899092/3‑143 (MQ=255) tccAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCag < 1:747432/141‑1 (MQ=255) agaCTATGCCACCACGCCAGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTctgtt < 1:274324/141‑5 (MQ=255) aaCTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAgaga < 1:900363/143‑1 (MQ=255) cTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTACCGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAgcgaca > 1:110575/1‑141 (MQ=255) tgCCACCGCGCCCGCAGCAGCGGCATCGGCCAGCGGTTTTTGCAGAGAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATgg < 1:252346/143‑1 (MQ=255) tgCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGATCTTTGCCGCTAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCGGCGTCAGAGAAACgg > 2:498496/1‑143 (MQ=255) ccaccaCGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGCTTTGCGGTGACGGATAATGCTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGAAGCTGGGCAGCGTCAGAGAAATGGCg > 1:888806/1‑143 (MQ=255) cacgacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGa < 2:670861/143‑1 (MQ=255) caccacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGa > 1:435050/1‑143 (MQ=255) cGCCCGCAGCAGCGTCATCGTCCAGCGTTTTGTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAAcc < 2:523942/143‑1 (MQ=255) tCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTaaaaa > 1:81708/1‑143 (MQ=255) cATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAAc > 2:733263/1‑143 (MQ=255) tCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCTGTGACGGATCATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGc < 2:117548/143‑1 (MQ=255) aGCGTTTTTTGCAGATAAACCGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCgcc > 1:316992/1‑141 (MQ=255) gCGGTTTTTGCAGATAGACAGTGATTTGCGGTGACGGATAATTCTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACATACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATTACCGTTAAAAACGTGGCGAAc < 2:417054/143‑1 (MQ=255) tttttGCAGATAGACAGTGATTTGCGGTGACGGATAATACTGGGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGtt < 1:887098/143‑1 (MQ=255) aTAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAACCGTGGCGAACGGTTTGCTTTTCa < 1:28043/143‑1 (MQ=255) aTAAACAGTGATTTCCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTGGTACACCATATAGCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCa < 2:519789/143‑1 (MQ=255) aGTGATTTGCGGTGACGGATAAGACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCt < 1:727355/143‑1 (MQ=255) gTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTg < 2:421589/143‑1 (MQ=255) gTGAGTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTg < 1:878537/143‑1 (MQ=255) tGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGCCGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGc < 1:572565/143‑1 (MQ=255) cgttGCGGGCACGGATAATCCTTCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGGTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCaa < 1:512230/141‑1 (MQ=255) tGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGt < 1:733263/143‑1 (MQ=255) tGACGCATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCCTATAACAGACGCTGGCCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGt < 2:672063/143‑1 (MQ=255) gATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAg < 1:151812/143‑1 (MQ=255) cgggTTCACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGt < 2:428974/141‑1 (MQ=255) ttttGTACACCATATAGCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGTAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTg < 2:84928/143‑1 (MQ=255) ttttGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTg < 1:303975/143‑1 (MQ=255) ttttGTACACCAGATAACAGCCGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCCCCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGCTCCTGCGATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTg < 2:84175/143‑1 (MQ=255) ttGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACggtggt > 1:233454/1‑143 (MQ=255) acCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCg > 1:45921/1‑143 (MQ=255) ataACAGTCGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCTCCTGTCAGTTGAAAACGTTGGTTTTGCGATTTa < 2:110575/143‑1 (MQ=255) aaCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGCTTTAcc > 1:484337/1‑143 (MQ=255) tctgACGCTGCCCAGGGTCAGAGAAATGGCGATAACCATCACCGTTCAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCACTGCGCCGTGGAAGGCCTAGCGCACCTGTTCGTTGAAACCGTTGGTCTTCCGAATTAcag > 2:410705/4‑141 (MQ=255) tctgACGCTGCCCAGCGTCTGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGCGGAAGCCATAGCGCCCCAGTACGTTGAAAACGTTGGTTTTGCGAtttcacg > 2:100579/4‑139 (MQ=255) tctgACGCTGCCCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGCTGCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGTTTTACCg > 1:247036/4‑143 (MQ=255) aCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTAACg > 1:242353/1‑143 (MQ=255) cGCTGGGCGGCGTCAAAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGtttt < 1:899092/143‑1 (MQ=255) gCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGGTTGCTTTTCAGATCCTGCAATGCGCCGTGGACGGCATAGCGCACCTGTTCGTTGAGAACGTTGGTTTTGCGATTTACCGGTtgtg > 1:348080/1‑143 (MQ=255) cgtGGGCAGCGCCAGCTAATTGGCCATAACGATCACCGTTAACAACGTGGCGAACGGTTTTCTTTTCATATTCTGCAATGCGCCTTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTg < 2:84348/141‑1 (MQ=255) gCAGCGTTCGATAAAGGGCGATCACCATCACCGTTAAAAACGTGGCGAACGGGCTGCCTTTCAGATCCTGCAATGCTCCGTGGAAGGCATAGCTCACCTGTTCGTTGAAAACGTCGGTTTTGCGATTTACCGGTTTTGGCgcg < 1:70031/143‑3 (MQ=255) gCAGCGTCATGGTAATGTCGATACCCATCACCTTTGAAAAGGTGGAGAAGGGTTTGGTGTGCAGATCCTGCAATGCGCCGGGGAAGGCATAGCGCACCGGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAg < 1:147482/143‑1 (MQ=255) | TCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAG > NC_000913/3601686‑3601967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |