| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 533,846 | T→C | 8.3% | intergenic (+20/‑70) | allR → / → gcl | DNA‑binding transcriptional repressor AllR/glyoxylate carboligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 533,846 | 0 | T | C | 8.3% | 140.3 / 3.3 | 58 | intergenic (+20/‑70) | allR/gcl | DNA‑binding transcriptional repressor AllR/glyoxylate carboligase |
| Reads supporting (aligned to +/- strand): ref base T (22/29); new base C (3/2); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01 | |||||||||||
ATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTG > NC_000913/533707‑533988 | gtctccctctccGGGGCGCCAGCGTGACTGTCATGCGAGCGTTTGCTCAGTCAGGGGGAGCTGGCCAGAGACACCGCCCGCGATATCAGCACGGCGTTGTGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCg < 2:478992/142‑1 (MQ=255) ctccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCCCTGCGGa > 1:476117/1‑143 (MQ=255) tccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAg > 2:557786/1‑143 (MQ=255) catctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGTGACACCGCCCGCGAGATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCg < 1:737493/143‑1 (MQ=255) atcgccGGGCCTCGATCAAGACTGCCAGAAGATCGTTTTGTCAGTCAGGGTGGGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCgg < 2:217123/143‑1 (MQ=255) ggcTTCATCAAGACTGACATAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGttttttt < 1:200856/141‑1 (MQ=255) ccTTCATCAAGTCTGACAGACGCTCGTTTGGTCAGTCAGCGTGAGCTGTTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTg < 1:350579/143‑1 (MQ=255) cTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACCTCCCTCATGTCTGTCGCCTCCCGCTCTTCGGAGCGGGATTTATTGa > 1:632522/1‑143 (MQ=255) aggatGACAGAAGATCGTTGTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAttt < 1:221745/139‑1 (MQ=255) aCAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACTAAATTTGATAGt > 2:520160/1‑143 (MQ=255) ctgaggctCGTGTTGTCAGTCTGGGTGAGCTGGTCAGAGACACCGCCCGCGATAGCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGtt < 2:485886/136‑1 (MQ=255) cAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGtt < 1:730709/143‑1 (MQ=255) cAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCAGCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGtt < 2:81542/143‑1 (MQ=255) aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTCGAACGTTGGaaaa > 2:838024/1‑143 (MQ=255) gTTTTGTCAGTCAGGGTGTGCTTGTCAGAGGCTCCGCCCGCGATATCAGCACGGCGTGGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAAtt < 2:376797/143‑1 (MQ=255) ttGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc < 1:557786/143‑1 (MQ=255) ttGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc < 1:276513/143‑1 (MQ=255) ttGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc < 2:465340/143‑1 (MQ=255) agtcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaata > 1:392874/1‑143 (MQ=255) tcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAACATTTTCCaataca > 2:32780/1‑141 (MQ=255) tcagAGTGAGCTGGGCGGGGACACCGCACGCGATATCAGCACGGCGTGGGGACTGAAAGCACATCCATGATGTCTGTCGCAGGCCGCTTTGCGGAGCGGGTTTTTTTGTCAAAATTTGAAAGTTGGAAAAATTTTCCaataaa < 2:763035/143‑1 (MQ=255) agGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaataaata < 1:211652/143‑1 (MQ=255) gTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAACAATTTTCCAATAAATagag > 2:577709/1‑143 (MQ=255) gTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATagag > 1:825735/1‑143 (MQ=255) gCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAg < 1:439371/143‑1 (MQ=255) ggcccgagtCACCGGCCGCGATATCCGCCCGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGaa < 2:510396/138‑1 (MQ=255) gTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAACTTTGAAAGTTGGAAAAATTTTCCAATAAATAGCGGTAGGAAt > 1:263715/1‑143 (MQ=255) gagaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATaaaa < 1:681369/143‑1 (MQ=255) gcgATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAGATGAAAAAGGGAAGCATTTTCCAATACATAGAGGAACGAACACAATGACAAAAGTga > 2:50290/1‑143 (MQ=255) tatCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTGTGACCACATTTGAAAGTTGTAAAAATTTTCCAATAACTGAAGATAGGAAAAAAATGGCAAAAATgaacac > 2:202015/1‑139 (MQ=255) aGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGtt > 1:245257/1‑143 (MQ=255) aGCACGGCGTGGGCACTGAAAGCGCATTCATAATGTCTGTCGCATCCCGCTCTGCGGGGCTGGTTTTTTTGACAAAATTGGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGtt < 2:176711/143‑1 (MQ=255) ggCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATGTGAAAGTTGGAAAAATTTTCCAATAGGTAGAGGTAGGAGTAAAATGGCAAAAATGGGAGCCGTTGTcgc > 1:245357/1‑143 (MQ=255) gcttcgggACTGAAAGCACATCTATAATGTCTCTCGCATCCCGCCCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGAcgcg < 1:885179/138‑1 (MQ=255) gttgctgcTGGAAGCCCCTCCATAATGTCTGGCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAGTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGCAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGc < 2:162797/136‑1 (MQ=255) gTTGAGACTGAAAGCATATCCATAATGTCTGTCGCATCCCGCTCGGCGGAGCGGGTTTTTTTGACAAAAGTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGc < 2:67485/143‑1 (MQ=255) gggATTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGTGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAat < 2:278128/143‑1 (MQ=255) gACTGAAGGGACATCCATACTGTCGGTCGCACCCCGCTCCGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAAGTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAatgt < 1:654075/143‑1 (MQ=255) acaTCCATAGTGGCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGga < 2:583369/143‑1 (MQ=255) aTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAATTTTGATAGTTGGTAAATTTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGgaga > 1:406030/1‑143 (MQ=255) aTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGCATGTGCTGgaga > 1:731046/1‑143 (MQ=255) cATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAGATTTTCCAATAAATAGAGGTAGGCATAAAATGGCAAGAATGAGAGCCGTTGACGCGGCAATGTATGTAATGGagaaaa > 1:59527/1‑142 (MQ=255) tAATGTCTGTCGGATCGCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAAGGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaaagaa < 1:89680/143‑1 (MQ=255) tAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTAGGGAAGATTTTCCATTAGATATAGGTGGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCACTGTATGTGCTGGagaatgaa > 2:846253/1‑143 (MQ=255) tAATGTCTGTCGCATACAACTATGCAGATCGGGTTTTTTTGACAAAATTTGAAAGTTGAAAAAATTTTCCAATAAATAGAGGAAGGAATAAAATGGCAAAAATGAGAACCGTTAACGCGACACTGAAGGTGCTGAagacagaa > 2:555821/1‑143 (MQ=255) aTGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAgg < 1:142504/143‑1 (MQ=255) aTGTCCGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAgg < 2:404841/143‑1 (MQ=255) aTGCCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAgg < 2:404856/143‑1 (MQ=255) tgtctgtcGAATCCCGCTCTGGGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGt < 2:338049/143‑1 (MQ=255) tctgtcGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGCAAGCTGGAAACATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAGTGAGAGCCGTTGACGCGGCACTGTATGTGCTGGAGAAAGAAGGTaa > 2:178580/1‑142 (MQ=255) ctgtcGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAGGTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATc < 1:357543/143‑1 (MQ=255) tgtcGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGCGAAAGAAGGTATCa > 2:495739/1‑143 (MQ=255) tgtcGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCa > 2:570582/1‑143 (MQ=255) aaaaaaacccGCTCCGCAGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAAGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTAGAGAAAGAAGGTATCAc > 2:158431/8‑143 (MQ=255) aaaaaacccGCTCCGCAGAGCGGGTTTTTTTGACAACATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATCCAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTCCTGGAGAAAGAAGCTCTCACt > 1:843430/7‑143 (MQ=255) tCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGc < 2:750553/143‑1 (MQ=255) cccGCTCCGCAGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGGAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGACGGGAGCACTCCCGcc > 2:262568/1‑143 (MQ=255) gCTCGGCGGAGAGGGTTTTATTGACAAAATTTGAAAGTTGGAAATGTTTACCAATAAAAAGAGGAAGGATGATGAGGGCAAAAATGAGCGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTc < 1:712033/143‑1 (MQ=255) ctctGCGGAGCGGGTTTTTTTGACAAAATCTTAATGTCGTATGAACTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCg < 1:617093/143‑1 (MQ=255) ctctGCGGAGCGGGTGTTTTTTACAAGATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAACTGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCg < 2:502677/143‑1 (MQ=255) tGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTACAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGgtg > 1:358353/1‑143 (MQ=255) | ATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTG > NC_000913/533707‑533988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |