| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,642,358 | T→A | 9.8% | intergenic (+27/+22) | dtpB → / ← rsmJ | dipeptide/tripeptide:H(+) symporter DtpB/16S rRNA m(2)G1516 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,642,358 | 0 | T | A | 9.8% | 126.7 / 7.1 | 51 | intergenic (+27/+22) | dtpB/rsmJ | dipeptide/tripeptide:H(+) symporter DtpB/16S rRNA m(2)G1516 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (23/23); new base A (2/3); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01 | |||||||||||
CCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTC > NC_000913/3642216‑3642500 | ccGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGt > 1:64905/1‑143 (MQ=255) tCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTCATTATTCTTGCAGAAAGCCGGGGAGCGTTAt > 2:156944/1‑143 (MQ=255) tCTATACCAACGTGTTGGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTAt < 1:32290/143‑1 (MQ=255) cagcttgtgtgGTAAGATTGGTCTGGTCACGTTGCGCGTCGCAGGAGTGATGCTGTTGAGGGTGCCGTGGCAGAAACGCATGATTGCGACGCCGGAAAGCCTTTAATTATTCTTGCAGAAAACAGGGTAGCGATAACGCTAcc < 1:357354/138‑1 (MQ=255) cAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTTCAGAAAGCAGGTTATCGTTATCGCTAcc > 1:1682/1‑143 (MQ=255) acttgtTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTTAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTACCCt < 1:646813/140‑1 (MQ=255) tGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTACCCTgctttct < 2:776048/143‑3 (MQ=255) tAAGAGGGGGCTGGTCGCGCTACACGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGTCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagttt < 1:612350/143‑1 (MQ=255) aGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtttta < 1:586283/143‑1 (MQ=255) aTTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTGCCCTGTTTTAGTTTTaca > 1:440450/1‑143 (MQ=255) aTTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTaca > 1:54032/1‑143 (MQ=255) tggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGt > 1:375045/1‑143 (MQ=255) cACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGCAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGATTTACACTGGCGTACCt > 1:514239/1‑143 (MQ=255) cTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAACGCGCAAGAGGAGCGTTGAGGTAAATATGGTACCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCAt > 2:646510/1‑143 (MQ=255) tGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGGTTTAGTTTTACACTGGCGTACCTGCATa > 1:438001/1‑143 (MQ=255) gCGTTGCAGTAGTGGTGCTGTGTCTGGGCCCTTTGCTGAAGCGCATGATTGCGACGGCGGAATGCCATTAATTATTCTTGCAGAAAGTAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGat < 2:7540/143‑1 (MQ=255) gCGTTGCAGTAGTGATGCTGTTGAGGCTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGat < 1:181603/143‑1 (MQ=255) cGTTGCAGTAGTTATGCTGTCGATGGGGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGata < 1:892772/143‑1 (MQ=255) gTTTCAGTAGTGATGCTGTAGCGTGTCCCGTGGCGGAAACGCACGACTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGatat < 2:221593/143‑1 (MQ=255) gTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGCAAGCAGGGTAGCGTTATCGCTCCCCTGTTTTAGTTTTTCTCTGGCGTATCTTCAGAGagat > 1:174018/1‑143 (MQ=255) gCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAAGTATTCTTGCAGAAAGCAGGGCAGCGTTGTCGCTACCCTGTTATAGTTTCACACGGACGTACCTGCCCAGATATaaa > 2:750416/1‑143 (MQ=255) cAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCaaa < 1:271013/143‑1 (MQ=255) agggATGCTGTTGATGGTGGCGTGGGTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGa < 2:539460/140‑1 (MQ=255) aTGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGcc > 1:78039/1‑143 (MQ=255) tGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGAGAGCGTTATCGCTACCCTGTTTTAGTTTGACACTTGCGCACCGGCATAGAGACCAACACGAGGCACttt > 2:187776/1‑143 (MQ=255) gggggtggtggCGTGGCTGAAACGCAGGTGTGCGACGCTGGAAAGCCATTCATGATTCTTGCAGAAAGCAGGGTAGCGTGATCGCTGGCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTa < 2:226795/138‑1 (MQ=255) tGAAGGTGCCGTGGCGGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGt < 1:542599/143‑1 (MQ=255) taaggTGCCTTGGCTTAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTg < 1:71308/140‑1 (MQ=255) tGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGGAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGAc < 2:160853/143‑1 (MQ=255) gTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTCTTCTTGCGGAAAGCAGGGGAAGGATGTCGCTATCTTGTTTTAGGTTTACACGGGCGTACCTGCATGGATATCAAAGTGATGCGCATTAGTgccga > 2:796469/1‑139 (MQ=255) tGCCGTGTCTTAAAGGCATGATTGCGACGCCTGAACGCCATTACTTACTCTCGCAGAAAGCACGGTAGCGTTATCGCTACCCTTTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTAc < 2:250698/143‑1 (MQ=255) ggtGAAACGCAGGAATGGGACGGCGGAAAGCCATTAAGTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTTTTTTTTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGttt < 1:126721/141‑1 (MQ=255) aCGCATGATTGCGAAGCAGGAAAGCAATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATCTCAAAGCGATGCCCCTTAGGGACTACCGCGTAtggcgtg > 2:373136/1‑143 (MQ=255) tGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGAc > 1:611043/1‑143 (MQ=255) ttGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCATACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACAtt < 1:884435/143‑1 (MQ=255) gcGGGAAGCTTTTAAGTATTCTTGCAGAAGGCATGGTAGCGTTATCGCTTCTTTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCg < 2:270155/142‑1 (MQ=255) ccGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTATGGCGTGGCGACATTCGCCAGCg > 2:531871/1‑143 (MQ=255) ggAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCTCGGTAGTGACTACCGCGTCTGGCGTGGCGACATTCGCCAGCGGt > 2:787101/1‑143 (MQ=255) attaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTCGTGACTACCGCGTTTGGCGTGGCGACATGCGCCAGCGGTGGCGCGtg > 2:443705/1‑142 (MQ=255) attaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTa > 1:786143/1‑143 (MQ=255) tacctGCAGAAAGCAGGGAAGCGTTATGGCCACCCTGGTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTAGCGCGTTTGGCGTGGCGACATTCGCAAGCGGTGGCGCGTAGTCCGGgc < 1:447705/139‑1 (MQ=255) tCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGgcg > 2:582507/1‑143 (MQ=255) tCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGgcg > 1:351621/1‑143 (MQ=255) cTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGgcgc < 1:468101/143‑1 (MQ=255) ttGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCGGCGGTGGCGCGTAGTCCGGGTGCt > 1:90581/1‑143 (MQ=255) tGCAGTATGCAGGGTAGCGTTATCGCTTCTCTGTTTTAGTTTTCCACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGTGCGTAGTCCGGGCGCtt < 2:438001/143‑1 (MQ=255) gCAGAATGGAGGGTAGCGTTATCGCTACCGTGTTGTAGTTTTGCACTGGCGTACCTGCATAGATATCAAAGAGCTGCTCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCttt < 1:818119/143‑1 (MQ=255) gAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTGCACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTacc < 2:1682/143‑1 (MQ=255) ggTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTAGCGTGTGGACATTCGCCAGCGGTGGCCCGATGAACGGGCGCTATACCACCActcg > 2:637319/1‑143 (MQ=255) ggTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGGTTGGCGTGGCGACATTCGCCAGCGGTGGCGCCTAGTCCGGGCGCTTTCCCGCCAagcg > 2:410529/1‑143 (MQ=255) ggTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCAcgcg > 1:538848/1‑143 (MQ=255) ggCGTTACCGCTACGCTGTTTGAGTTTTACACTGGCGTTCCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGttt < 2:387147/142‑1 (MQ=255) ttATCGCTGCCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTc < 2:375045/143‑1 (MQ=255) ttATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCACCGGTGGCGCGTAGTCCGGGCGCTTTACCCCCACGCGTTGGGTc > 2:602369/1‑143 (MQ=255) | CCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTC > NC_000913/3642216‑3642500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |