| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,269,395 | T→C | 7.0% | intergenic (‑383/‑19) | yjbS ← / → aphA | uncharacterized protein YjbS/acid phosphatase/phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,269,395 | 0 | T | C | 7.0% | 145.8 / 2.2 | 58 | intergenic (‑383/‑19) | yjbS/aphA | uncharacterized protein YjbS/acid phosphatase/phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base T (20/33); new base C (2/2); total (22/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01 | |||||||||||
AGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGC > NC_000913/4269255‑4269531 | aGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGACGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAACGTTTCAACATACTGa > 2:259224/1‑143 (MQ=255) ggAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATACAAAGTTTCAACATACTGAc > 2:253326/1‑143 (MQ=255) gTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTAttt < 2:740165/143‑1 (MQ=255) tATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCACCATACTGACTATTTa > 1:599714/1‑143 (MQ=255) tATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTa < 2:117823/143‑1 (MQ=255) agttagggggTGAAATATAATCATGCTGCTTGTGATCACAAAAGCCGCAGATAAGCCTTCCGTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTGTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAg < 1:227047/136‑1 (MQ=255) aTTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAg < 2:580863/143‑1 (MQ=255) aTTTACTGGGTTAAATATAATCACCCTGCTTTTCATCACAAAAACCGCATATAATCCTTCCTTTCCCCGGCAGCTGGCGTCATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAg < 1:146766/143‑1 (MQ=255) ttgccTGGCTTACATGTAACCCTCCTGCTTGTCATCACCAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACCAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAgg < 1:830490/139‑1 (MQ=255) tattataAACATCCTGTTTTTCAGCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATgc < 1:901960/140‑1 (MQ=255) aaTCCTCCTGCCTCTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAAGAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGa < 2:351631/143‑1 (MQ=255) gCTCTTCATCACAAAATCCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATTGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAgg < 2:565592/143‑1 (MQ=255) cTTGTCATCACAAAAAGCGCTGATAATCCTTGCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGc < 2:547841/143‑1 (MQ=255) ttttCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCa > 2:345658/1‑143 (MQ=255) tttCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCGCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCaa < 1:129798/143‑1 (MQ=255) ttCACCACAAAACCCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAAt < 1:552959/143‑1 (MQ=255) tcaCAAAAACCGCAGATAATCCTTCATTTCCCCGGCAGCTGGCGGTATGGTCAGATGGTTTTTGCAGCAACCCACACAATAACAAGTTTCAACATACTGACTATTTCGGGAGTAATCTGCGGAAGATGGCACGGGcagtcagt > 1:85307/1‑143 (MQ=255) cacaaaCCGCAGATAATCCTTCCTATACACGGCAGCTGGCGATAGGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGcc > 1:173301/4‑143 (MQ=255) ccGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGc > 1:451415/1‑143 (MQ=255) gATAATCCTTCCTTGCCCCGGCAGCGGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTAtt < 2:212614/143‑1 (MQ=255) aaTCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGtt < 1:566532/143‑1 (MQ=255) aTCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGACAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATCGTTc > 1:325267/1‑143 (MQ=255) gcttCCCCGGGCAACTGGCGTTATGGGCAGATGGGTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATTCTTACTATTTAGTGCCGAATATGCGCAAGACCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTa < 2:902237/142‑1 (MQ=255) ccttTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTa < 2:709873/143‑1 (MQ=255) tCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACa < 2:496227/143‑1 (MQ=255) cGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACCGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTc > 2:466423/1‑143 (MQ=255) cGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACCGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTc > 2:783719/1‑143 (MQ=255) aGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCt < 1:800926/143‑1 (MQ=255) cGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTCTTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGcc > 1:155791/1‑143 (MQ=255) ttATGGTCAGATGTTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCt < 1:299395/143‑1 (MQ=255) atATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCt < 1:637941/142‑1 (MQ=255) tATGTTTAGATGGTTTTTGCAACAAAGCACACAATAAAAAGTTCCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTg < 1:350932/143‑1 (MQ=255) cAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCa < 1:536460/143‑1 (MQ=255) gATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCAGTTTGATCAGCGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCAtc > 2:541215/1‑143 (MQ=255) tttttGCAACGAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCtt < 2:5270/143‑1 (MQ=255) tttttGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCtt < 1:399259/143‑1 (MQ=255) ttGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTAGTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCAc < 2:849038/143‑1 (MQ=255) tGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTCAGCAGTTCCGCTGTTGCGCTTGTCGCCTGTCCCTCAcc > 2:487386/1‑143 (MQ=255) aacaaATCTCACAATAAAAAGTTTCAACATACTGACTGTTGAGTGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTCGCCTCATCTCCTTCACCGCt > 1:673731/1‑143 (MQ=255) acaaATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCtt > 1:105533/1‑143 (MQ=255) ctcACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCt < 1:547162/143‑1 (MQ=255) tcACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTg < 2:241439/143‑1 (MQ=255) cacaATAAAAAGTTTCCACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTgg < 1:333436/143‑1 (MQ=255) cacaATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGGCGTTGCCCTGGCCTCATCCCCTTCACCGCTTAACCCTgg > 2:443959/1‑143 (MQ=255) acaATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTggg < 2:130982/143‑1 (MQ=255) acaAGAAATAGGTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTggg < 2:583418/143‑1 (MQ=255) tAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAAGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTa > 1:475007/1‑143 (MQ=255) aaaaGTTTCAACATACCGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAAc < 1:783719/143‑1 (MQ=255) aaaaGTTTCAACATACCGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAAc < 1:466423/143‑1 (MQ=255) aaaaGGTTCCACATACGGACTATGTAGGGAAAAATATGCGCAAGATCACACAGGCAAGCAGTGCCGTTTGCTTATTGTTCGCTCGAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAAc < 1:92082/143‑1 (MQ=255) aaaGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTCACg > 1:269621/1‑143 (MQ=255) aattttCCACATACTGACTATTTAGGGAAAACTATGCGCAAGATCACACCGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGTCTCATCTCCTTCTCCGCTTAACCCTGGGACTAACGt < 2:251201/140‑1 (MQ=255) aaGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTCACGt > 1:409776/1‑143 (MQ=255) ttCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGCCTAACCTTGcc > 2:365125/1‑143 (MQ=255) tCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCCTCTCCTTCACCGCTTAACCCCGGGACGACCGCTGCCa > 2:678379/1‑143 (MQ=255) gaCATCCTGACTATTTAGGGAAAAATATGCGCAAGCGCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAgg < 2:412323/142‑1 (MQ=255) aCATACTGACTATTTAGGGAAAACTATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGc < 1:259224/143‑1 (MQ=255) aCATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTCACGTTGCCAGGc > 2:72654/1‑143 (MQ=255) | AGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGC > NC_000913/4269255‑4269531 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |