Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,367,702	A→C	8.8%	I209S (ATC→AGC)	aspA ←	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,367,702	0	A	C	8.8%	157.1 / 4.8	65	I209S (ATC→AGC)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (24/34);  new base C (3/4);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

TTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCA  >  NC_000913/4367563‑4367840                                                                                                                                            |                                                                                                                                           ttCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGc                                                                                                                                         >  1:495129/1‑143 (MQ=255) ttCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGc                                                                                                                                         >  1:830214/1‑143 (MQ=255)     gCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGaa                                                                                                                                     <  2:655133/143‑1 (MQ=255)      ccAGTTTGTTCACTGCGAGCGGAGAGTACTCTTTCGGCTTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGaaa                                                                                                                                    <  2:14527/143‑1 (MQ=255)      ccAGTTCTTTCACTGCCAGCGGAGAGTACGCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGaaa                                                                                                                                    <  1:222957/143‑1 (MQ=255)      ccAGTGTTTTCAGGGCCGGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTCACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGaaa                                                                                                                                    <  2:389035/143‑1 (MQ=255)       cAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAg                                                                                                                                   <  1:42167/143‑1 (MQ=255)       cAGTTGTGTCACTGCCAGCGGAGAGTACGCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAg                                                                                                                                   <  2:620251/143‑1 (MQ=255)          ttttttCACTGCCAGCGGCGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaag                                                                                                                                <  1:56538/143‑4 (MQ=255)             tttCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGCTGCTGAAAGCGCGGa                                                                                                                             >  1:639336/1‑143 (MQ=255)              ctcgcTGCCAGCCGAGCGTACGCTTTCGGCGTGTTCAGGCCAGTACCGATTGCTGGTGCACCAGGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAGGCGCGGaa                                                                                                                            <  2:859613/139‑1 (MQ=255)                 aCTGCCAGCTGTGAGTACTCTTTCGGCGTGCTCAGACCAGTTCCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTc                                                                                                                         <  2:483609/143‑1 (MQ=255)                 aCTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCTGTACGTTGTATGTTGTTGGTTTCTGCCTTCTTCATGATGCTGAAAGCGCGGAATTc                                                                                                                         <  2:66553/143‑1 (MQ=255)                    gCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTGAGCAGGATGCTGAAAGCGCGGAATTCCTg                                                                                                                      >  1:85128/1‑143 (MQ=255)                     cgaGCGGAGAGTGCGCTTGCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGa                                                                                                                     <  1:116737/141‑1 (MQ=255)                      cAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGAc                                                                                                                    >  1:432184/1‑143 (MQ=255)                        gCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCCCGGTTAACTTCCAGCAGCCGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCg                                                                                                                  >  1:545432/1‑143 (MQ=255)                                tACTCTTTCGGAGTGTTCATACCAGTACCGAGTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCAt                                                                                                          <  2:140888/143‑1 (MQ=255)                                      gtCGGCGTGGTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTGCGATGACATTCATTGCGTCTTTCAGCAGGAGGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTAc                                                                                                    <  1:209451/142‑1 (MQ=255)                                         ggCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTAAAAGCGCGGAATTCCTGACCGAGGGTCATCGATACtac                                                                                                 >  1:306527/1‑141 (MQ=255)                                            gtgtTCAGCCCAGTACCGGTTGCGGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTc                                                                                              <  2:774561/143‑1 (MQ=255)                                            gtgtGCAGACCAGTACCGATTGCTGTTGCACGAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTGTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTc                                                                                              <  2:631619/143‑1 (MQ=255)                                               ttCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGCTGCTGAAAGCGCGGAATTCTTGACCGAGGGTCATCAGTACTGCGTCCTg                                                                                           >  1:524998/1‑143 (MQ=255)                                               ttCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGCCCGAGGGTCATCGGTACTGCGTCCTg                                                                                           >  1:688090/1‑143 (MQ=255)                                               tgcAGACCAGTACCGATTGCTGTTGCACCAAGGTGAGCTTCCAGCAGCAGTTCAGCGGTACGTGGGATGTGTTTCACTGCTTCTTTCAGCGGGATGCTGAAGGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTg                                                                                           <  2:212500/141‑1 (MQ=255)                                                tCAGACCAGTGCCGGTTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGc                                                                                          <  1:396175/143‑1 (MQ=255)                                                tCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGc                                                                                          <  1:687449/143‑1 (MQ=255)                                                      cAGTACCTTTTGCTGTTTCCCCAAGTTTTACTTCCAGCAGCAGTTCAGCTGTACGTTGGATGTTTTTCTCTTTTTCTTGCAGCAGGATGCTGAAAGCGCTGATTTCCTGACCGAGGGTTATCTGTACTGCGTCCTGCAGCTgg                                                                                    <  2:73985/143‑1 (MQ=255)                                                      cAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTgg                                                                                    <  1:429298/143‑1 (MQ=255)                                                      cAGTACCGAGTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTgg                                                                                    <  1:886617/143‑1 (MQ=255)                                                      cAGGACCGATTGCTGTTGCACCAAGGTTAACTTCCATCATCAGTTCAGCGGTACGTTTGTTGTTTTTCACTTCTTATCCCGTGTGGATGCTGAAAGCGCGGAATTCCTGTCCGAGGGTCATCGGTACTGCGTCCTGCAGCTgg                                                                                    <  2:599456/143‑1 (MQ=255)                                                         tACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGTGTa                                                                                 >  1:321862/1‑143 (MQ=255)                                                         caCCGAGTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTa                                                                                 <  1:656176/142‑1 (MQ=255)                                                          aCCGATGGCTGCCGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTGTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTAc                                                                                <  1:229985/143‑1 (MQ=255)                                                            cGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCCGGCTGCTGAACGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGa                                                                              >  1:98235/1‑143 (MQ=255)                                                                       gCACGAAGGTTAACTTCCGGCGGCAGTTCGGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCGGAAAGCGCGGATTTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAg                                                                   <  2:210181/143‑1 (MQ=255)                                                                           cAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTAGGACCCATTTTCAGGaag                                                               >  1:410743/1‑141 (MQ=255)                                                                             aGGTTAACTTCCAGCGGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTc                                                             >  2:771801/1‑143 (MQ=255)                                                                                 tAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCCGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCCGCTGGGTACGAGCCCGTTTCAGCATGCCCggg                                                         >  2:731196/1‑143 (MQ=255)                                                                                 gtacctCCAGCAGCCGGTCAGCGGTATGTTGGATGCTTTTCGCTTCTTCTTTCAGCAGGAGGCTGAACGCGCGGAATTCCTGACGGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGGACGACCCATTTTCAGGATGTCCTgg                                                         <  1:361280/138‑1 (MQ=255)                                                                                  aaCTTCCGGCAGCAGTTCAGCGGGACGTTGGATGTTTTTCACTTCTTGTGTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGa                                                        <  1:654145/143‑1 (MQ=255)                                                                                  aaCTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGa                                                        <  2:875710/143‑1 (MQ=255)                                                                                     ttCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAAtt                                                     >  1:184228/1‑143 (MQ=255)                                                                                     ggcgagcagcagGTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAAtt                                                     <  1:493688/139‑1 (MQ=255)                                                                                        cagcagcagTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGa                                                  <  1:892523/143‑1 (MQ=255)                                                                                          gcagtcgTTCAGCGGTACGTTGGATGTTTTTCGCTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACa                                                <  1:897137/137‑1 (MQ=255)                                                                                             gcagTTCAGCGGTACGTTGGATGCTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCt                                             <  1:379640/143‑1 (MQ=255)                                                                                                 ttCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATGCGACAGCTTTAc                                         >  1:179631/1‑143 (MQ=255)                                                                                                    aGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGATTAATGCTCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCCTTTTCCGGCTGTCCTGGAATTCGCCCGCTTTCCGtg                                      >  1:468704/1‑142 (MQ=255)                                                                                                       ggTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGCCCTGGAATTCGACAGCTTTACGTTCac                                   >  1:393141/1‑142 (MQ=255)                                                                                                        gTACGTTGGATGTTTTTCACTTCTTCTTTCAGATGGATGCTGAAAGCGCTGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCGGGATGTCCTGGAATTCGACAGCTTTACGTTCaaa                                  <  1:909744/143‑1 (MQ=255)                                                                                                         tACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCCGGAGGTCCTGGAAGTCGACAGCGTGACGGTCAAAg                                 >  2:470351/1‑143 (MQ=255)                                                                                                             ttGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCtt                             >  2:506323/1‑143 (MQ=255)                                                                                                             ttGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCtt                             >  2:497460/1‑143 (MQ=255)                                                                                                                  tGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGCCCCATTTTCAGGATGTCCTGGAATTCGACAGATTTACGTACAAAGCCTTCACGc                        >  1:829416/1‑143 (MQ=255)                                                                                                                     ttttCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGt                     <  2:830214/143‑1 (MQ=255)                                                                                                                     tgttCACTTCTTCTTTCAGCATGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGt                     <  2:495129/141‑1 (MQ=255)                                                                                                                       ttCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATATTTTGGAAGTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTg                   >  1:513046/1‑143 (MQ=255)                                                                                                                          aCTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGtt                >  2:6294/1‑143 (MQ=255)                                                                                                                          aCTTCTTCCTGCAGCAGGATGCTGAAGGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGtt                <  1:394174/143‑1 (MQ=255)                                                                                                                            ttcttcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTCCACGCCGTTGGTTaa              >  2:576452/1‑143 (MQ=255)                                                                                                                                       gcagGATGCTGAAAGCGCGGAATTCCTGCCCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTAcc   <  1:312837/143‑1 (MQ=255)                                                                                                                                        cagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTAGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCa  >  1:178621/1‑143 (MQ=255)                                                                                                                                           gATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCa                       >  2:168136/1‑119 (MQ=255)                                                                                                                                           gATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCa                       <  1:168136/119‑1 (MQ=255)                                                                                                                                            |                                                                                                                                           TTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCA  >  NC_000913/4367563‑4367840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑