| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,472,437 | T→C | 9.3% | N33D (AAT→GAT) | pyrL ← | pyrBI operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,472,437 | 0 | T | C | 9.3% | 120.8 / 5.0 | 54 | N33D (AAT→GAT) | pyrL | pyrBI operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base T (31/17); new base C (3/2); total (34/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-02 | |||||||||||
GCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAA > NC_000913/4472298‑4472577 | gCTTTCATTTCCGCCGCTGTCGCCAGCACCAGGTTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCCGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAg < 2:588878/143‑1 (MQ=255) ttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAgggg > 1:761607/1‑143 (MQ=255) cAGTTTCGCCGCTGTGGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCt < 2:610709/143‑1 (MQ=255) tttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCCggg > 1:292298/1‑143 (MQ=255) tttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCCCCTGACGCCTGGGCAAGAAAAAGCCCCTCGATTGGGGGGCgggg > 1:615599/1‑143 (MQ=255) tCGCCGCGGTCGCCAGCACCAGAGTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCtttttt < 2:569819/143‑6 (MQ=255) cgccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAAt > 1:676334/1‑143 (MQ=255) gcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTCAGGTCGTTTATGCAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGt < 1:59309/143‑1 (MQ=255) ggcGCCCGTACCAGATTAAGGGCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAt < 2:776614/141‑1 (MQ=255) agaaacAGATTAAGGTGATCGCGACGAAGGCCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCGTTTATCTCCGGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACgg < 2:690760/138‑1 (MQ=255) aGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAACACgg > 2:152856/1‑143 (MQ=255) gTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCACCTCGATTGAGGGGGTGGGAATGGGAGAGCAACGGGAAGAACAACGGCa > 2:433150/1‑143 (MQ=255) gTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCAATCTTTTAGCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCACCGCAACGaaaaaggcca > 2:262235/1‑138 (MQ=255) ggatgaaGGGCGGTTATGGAAATGATATGTGTCTGATATAGTGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGc < 2:352474/138‑1 (MQ=255) gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGGGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑ttttt > 1:567659/1‑143 (MQ=255) tttATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGAGGCCTGGGCAAAAAAACGCCCCTCGATTGAGGGGCTGGGGATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAg > 1:693474/1‑143 (MQ=255) aTGGAAAGGATGTGGCTCTGATATAGGGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGAcg < 2:209198/143‑1 (MQ=255) gAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCgg > 2:701327/1‑143 (MQ=255) aaTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCCGCGTC‑TTTTTTCAGACGCGGTa > 1:35033/1‑143 (MQ=255) ttatatGTCTCTGGTATAGCCGATTAGCCATCTTTTATCTCCTCACGCCTGCGC‑AAGAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAGCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGa < 2:337471/141‑1 (MQ=255) atatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCATGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGAGGCGGTAAGAc > 2:127540/1‑143 (MQ=255) tatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACa > 1:614294/1‑143 (MQ=255) tGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCg < 2:676334/143‑1 (MQ=255) tGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCg > 1:352504/1‑143 (MQ=255) gATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAGATGGCGa > 1:154160/1‑143 (MQ=255) ataGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGGCAAAATGTCGAaca > 2:434181/1‑143 (MQ=255) taGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGACCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAacac > 2:120324/1‑143 (MQ=255) gCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACGAAATGTCGAacacac > 1:511248/1‑143 (MQ=255) aTTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGaa > 1:735579/1‑143 (MQ=255) aGCCATCTTTTATCTCCTGACGCCTGGGC‑AAACAAAGCCCCTCGATTGAGGGGCTGGGACTGCGTGTATAACGTTAATAGCACTATACTTCTTGCTTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCAt < 1:421688/143‑1 (MQ=255) aTCTTTTATCGCCTGACTGCGGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAAAGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGAAATCATGTCGAACACACTGAACCATACa < 2:800828/143‑1 (MQ=255) tttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcct > 2:387216/1‑143 (MQ=255) aTCACCAGACGCATGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTccc < 1:629964/143‑1 (MQ=255) cTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGGGGGGCTGGGGATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCaa > 1:821046/1‑143 (MQ=255) gACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAACCTCCCGGCAAAt > 2:829600/1‑143 (MQ=255) gCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGt > 1:12428/1‑143 (MQ=255) ccTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAATAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTc < 2:446820/143‑1 (MQ=255) tGGGCAAAGACAAGCCCCTTGATGGAGGGACTGGGAATGGGTGAGCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑GTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCg < 2:367048/143‑1 (MQ=255) gggCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGACCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCgg < 1:120324/143‑1 (MQ=255) aaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATta < 2:352504/143‑1 (MQ=255) ttcccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTACGGCGCGGTAAGACAAAATCTCGAACACACTGAACCAGCCATCCTCCCGGCAAGTTGTCCGGCCTtat > 2:365522/6‑143 (MQ=255) ccaGCGCCTCAATCGAGGGGCTTGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCACCCGGCAAACTGTCGGGCATTATAc > 2:757790/3‑143 (MQ=255) aaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACt < 2:292298/143‑1 (MQ=255) caGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGTGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTc > 1:240256/2‑143 (MQ=255) aaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAACCGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATAAATCATCCCGGCAACTTGTCCGGCACTCTACTc > 2:624622/1‑143 (MQ=255) ccgcGATTGAGGGGCTGGGAGTGGGTGATCAACGGGAAGAAAAGCGGCAGGCCAGCGTC‑TCTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGt < 2:417221/140‑1 (MQ=255) ccTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATCCTCATCGt > 1:171434/1‑143 (MQ=255) cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTc > 1:808264/1‑143 (MQ=255) cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTAATAGTc > 1:149081/1‑143 (MQ=255) cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGAGTC‑TTTTTTTAGACGCGGTAAGACAAGATGTCGAACCCACTGAACCATACATCCTCCCGGCACATTGTCCGGCATTAGATTCAtgttc > 1:39163/1‑139 (MQ=255) tCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCCGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACAAACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCACCGACa > 2:96658/1‑143 (MQ=255) ccGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCa < 1:720394/142‑1 (MQ=255) gATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTCTACTCATCGTCAGa > 2:551951/1‑143 (MQ=255) aTTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGaa > 2:650846/1‑143 (MQ=255) | GCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAA > NC_000913/4472298‑4472577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |