| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 97.7% | 125.3 / NA | 44 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (18/25); minor base A (0/1); total (18/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCA > NC_000913/124101‑124380 | gtgctgGTCACGATCCGAATATAATCTATGCAGCATCCAAGAAAGCGGAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAg < 1:28058/143‑1 (MQ=255) tggtggTGACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAgg < 1:628437/143‑1 (MQ=255) gtggtCACGATCCGAAGAAAATCTACCCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACTGTAATCCTTGCTCATACCATTAAAGGTCACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGtt < 1:277130/143‑1 (MQ=255) gtCACGATCCGAAGAAAATCTGCGCTGCAGTGAAGAAAGCGCAGGAAACCAGAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAg < 2:146361/143‑1 (MQ=255) cACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCTCTCGGTTAAGaa > 1:887024/1‑143 (MQ=255) gATCCGAAGAAAATCTCCGCTGCATTCAAGAAAGCGCAGGAAACCATATGCAATGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAt < 1:84956/143‑1 (MQ=255) gaagaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGCCAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCCGAAGGTCAAAACATCGCGCACTACGTTCAGAATATGcaca > 1:618215/1‑143 (MQ=255) aagaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt > 1:967603/1‑143 (MQ=255) cTACGCTGCATTCAAGAAAGCGAAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtg < 1:163069/143‑1 (MQ=255) cGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTCGGTTAAGAAAATGAACATGTACGGTGTGc > 1:354581/1‑143 (MQ=255) cTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGGAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCCGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGt < 1:63890/143‑1 (MQ=255) aaTTCAAGAAAGCGCAGGAAACCAAAGGCAACGCGACAGTAATCCTGGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCat < 2:317703/142‑1 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAACGTTACGCCATGGCCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTAAAGAAAATGAACATGGACCGTGTGCGTCACATCcg > 2:1079229/1‑143 (MQ=255) gAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgcg > 1:205879/1‑143 (MQ=255) gcAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtgt < 1:610149/123‑1 (MQ=255) gcAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtgt > 2:610149/1‑123 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGACCATGGACGGTGTGCGTCATGTCCGCGTCCGTTTCaa > 1:192519/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCaa > 1:189416/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGGTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGTGTCATATCCGCGACCGTTTCaa < 1:3903/143‑1 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAACCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAACCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCaa < 2:18076/143‑1 (MQ=255) cAAGGGCAACGCGACAGCAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGc < 1:108041/143‑1 (MQ=255) cAACGCGCCAGTAATCCTTGCTCATACCATTAAAGGTTCCGGCCTAGGCGACGCGGCCGAAGGTCACAACATCGCGTACTCGGTTCAGAATGTGAACATGGACGGTATGCCTCATAACCGCGGCCCATGCAACGTGccctcgg > 2:711705/1‑138 (MQ=255) aaGCGACAGTAATCCTGGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCt < 2:61008/143‑1 (MQ=255) cAGTAATCCTTGCTCATACCATGAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCa < 2:356948/143‑1 (MQ=255) tAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGat < 2:935230/143‑1 (MQ=255) gtCCTTGCTCATACCTTTAAGGTTTACGGCATGGGCGAGGCGGCTGAAGGTAAAAACATCGCGCACTAGGGTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGatat < 1:1052566/142‑1 (MQ=255) ccTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCg > 1:101034/1‑143 (MQ=255) tcgCTGATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCTTTAGGTTAAGCAAACGAACATCGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaa < 1:1020131/141‑1 (MQ=255) tGCTGAGACCTTTACAGGGTACGGCATGCGCGACGCGGCTGAAGGTAAAAACACCGCGCACTAGGTTAAGAAAATGAACATGGACCGTGTGCGTCATATCCGGGACCGTTGCAATGTGCCGGTGTCTGATGCAGATATCGaaa < 2:629406/143‑1 (MQ=255) tGCTCATTCCATGAAAGGTGACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCGCTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaa < 1:385598/143‑1 (MQ=255) tGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCGATGTGCCGGTGTCTGATGCAGATATCGaaa < 1:303093/143‑1 (MQ=255) aTACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTCTCTGATGCAGATATAGagaaggcg > 1:300261/1‑139 (MQ=255) aTACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGATAAAAACAACGCGCACTAGGCTAAGAAAATGAACCTTGCCGCTGTGCGGCATATCCGCGACCGTTTCAATAAGCCGGCGTCTGATCCAGACATCGAAAAACTg > 2:331960/1‑143 (MQ=255) tACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGc > 2:719966/1‑143 (MQ=255) aCCATTAACAGGAACGGCAAGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGcc < 1:139212/143‑1 (MQ=255) cATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATACAGATATCGAAAAACTGCCGt > 1:957356/1‑143 (MQ=255) gaaaGGTAACGGCAGGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACa < 1:111622/142‑1 (MQ=255) gcgtGGGCGACGCGGCTCAAGCACGTAATATCTCCCACAAGGCCAGGGAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCg < 1:709616/140‑1 (MQ=255) gggCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAg < 1:355419/143‑1 (MQ=255) gCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGCTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGTTATCGACAAACAGCCGTACATCACCTTCCCGGCAGGCTCTGAcga > 1:1075421/1‑143 (MQ=255) ggTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATAc > 1:406676/1‑143 (MQ=255) tAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCt > 1:1027531/1‑143 (MQ=255) aaactgcgcgCACTGTGTTAAGAATGTGAATATGTACGGTTGGCGTCCGATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATc < 2:1055699/137‑1 (MQ=255) cACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCa < 1:314821/143‑1 (MQ=255) | GTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCA > NC_000913/124101‑124380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |