| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 95.5% | 125.7 / ‑5.3 | 44 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (22/20); minor base G (2/0); total (24/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA > NC_000913/2132648‑2132919 | ggtcgggTAGAGGGTAGACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAGAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct < 2:359637/138‑1 (MQ=255) gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg > 1:676556/1‑143 (MQ=255) tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:526734/143‑1 (MQ=255) ggTAGACAGCGGATATTGTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt < 1:136229/143‑1 (MQ=255) cTGATAGTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGTAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTcc < 2:921211/143‑1 (MQ=255) tGGCGCGGTTGAATTTGTCGTGGTGTTTGCAGTAAATGTGGAAGTGCCCTGAGAATTTTTTCGGTGACGATGTGATCTGCATTTCACCGTGATTGATATGCTGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTAc < 2:37566/143‑1 (MQ=255) tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTCATTGATATGCAGGCTCTGGGTCGCCTCTTCTAC‑TTTCCCCGGTTCGCCGtca > 2:764121/1‑141 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCgaccta > 2:44409/1‑138 (MQ=255) cctccgTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTc < 1:412212/138‑1 (MQ=255) cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTcc > 1:399101/1‑143 (MQ=255) gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTccacca > 2:789045/1‑143 (MQ=255) gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTccacca < 1:789045/143‑1 (MQ=255) cGATTTTGTCTTTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTccaccacc < 2:344989/143‑1 (MQ=255) gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTccaccacca > 1:727594/1‑143 (MQ=255) gTCTTTGCGCTCGCGCGGAAGGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCa < 1:151408/143‑1 (MQ=255) cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCTgg < 2:619329/143‑1 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTACACGGTTCGCCGTACACCACCACAATCCGCAGGa > 2:535890/1‑143 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCAGTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGCTCGCCGTACTCCACCACCACCCGCAGGa > 2:784539/1‑143 (MQ=255) tgtgCTTTCTTTAATATTGGTTGTACCCATAGAATTTTTTGGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg < 1:154545/143‑1 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGACGt > 2:968273/1‑143 (MQ=255) gggaaaaGTGGGCGTGCCCTAACAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa < 1:1067641/140‑1 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATGTGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACACCCACAGCGACCGCCGGACGCTGTGGTaac > 2:8629/1‑142 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGTGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTact > 1:779006/1‑141 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTGCGCCGTACTCCACCAACTACCGCCGGAAGAGGTCGTact > 2:332444/1‑141 (MQ=255) tcaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc < 1:662644/141‑1 (MQ=255) aaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCGCCATCCGCAGGAGGATGTCGTCATCCtg > 1:495790/1‑143 (MQ=255) gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCTTCCGCAGGACGATGTCGTAATCCtgtg > 1:397734/1‑143 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCCCCCTCCGCAGGACGATGTCGTCTTCCTGTGc > 1:245310/1‑143 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGAGACTCTATTACCTCTTTTACACGTCGAC‑AATGTCCGTACTCCACCACCATCCACAGTAAGACGTCGTAATCCtccgc > 2:1109380/1‑139 (MQ=255) ggACGTCCCCGGAGAATATTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGcc < 1:445163/143‑1 (MQ=255) ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGACTGCTATGCAGGCACTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGCAGCTGAAGAAATCCGTTGCCgacctcag > 2:539679/1‑136 (MQ=255) ggaggaTGTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt < 1:795345/143‑1 (MQ=255) aTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCGTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTa < 2:399101/143‑1 (MQ=255) ttttttCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGAGTCTGCGGCGCAGTTTCGAG‑ATTCCACGAATCGCCGTACTCCACCACCATCCACACGACGAAGTCGAAAGCCTGTGCCAATACCAGTCCGGTac > 2:886430/1‑142 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCTCTTCTAC‑TTTCCACGGTTAGCCGTCCTCCTCCACCCCCCGCAGGGAGATGGCGGACTCCTGTGCCGCTGTCAGCTCGGtgact > 2:941342/1‑139 (MQ=255) ggtCGGGGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGa < 1:135950/141‑1 (MQ=255) gACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTCTCCAGTCTGTATCGagaaaacc > 2:605249/1‑142 (MQ=255) ggATCTGCCTTTCCCCGTGATTGATATGCAGGCCCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGCACGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTg < 2:922984/142‑1 (MQ=255) gctCTGCCTTTCACCGTGAGTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa < 2:478947/141‑1 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTTCCGCTTTCAGTTCGGTATAGAACTAACACTCATTGacact > 2:182086/1‑142 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg > 2:483869/1‑143 (MQ=255) tgtctccGTGATTGATATGCGGGCTCTGCGTCGCCTCTTCTGC‑TTTCGCCGGTTCGCCGTGCTCCACCACCTGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt < 2:290144/138‑1 (MQ=255) aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACCCTTTGAAACGCCATGccc > 1:398501/1‑143 (MQ=255) gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGCCGTAATCCTGTGCCGCTTTCAGTTCGGTATGGAACAACAACCCCTTGAAACGCCATGCCCAgg > 2:803357/1‑143 (MQ=255) gatATGCAGGCTCTGCGTCGCCGCTTCTAC‑GTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa < 1:880182/143‑1 (MQ=255) | GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTAC‑TTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA > NC_000913/2132648‑2132919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |