Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	90.0%	101.0 / ‑2.3	41	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base T (18/18);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696332‑696606                                                                                                                                           |                                                                                                                                         tCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTAGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGAGTCGAAACTCGGAATGCCGGACTCAGag                                                                                                                                      >  2:455730/1‑142 (MQ=255)    cATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGCGGATGGGGTACGAGGATTCGACCCGCGGAATGCCGGAATTAGCATcc                                                                                                                                   >  2:291771/1‑143 (MQ=255)       cggagcttGAAGGGTAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAATGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                <  2:926746/137‑1 (MQ=38)         gccagtGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                              <  1:632289/138‑1 (MQ=255)            aTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCtt                                                                                                                           >  1:901878/1‑143 (MQ=255)                  ggATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAACAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAACCCGGTGCCTTACCGCt                                                                                                                     >  2:383869/1‑143 (MQ=255)                   gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATCAGTATGCTGTGCCTTACCGCtt                                                                                                                    >  2:445200/1‑143 (MQ=255)                   gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCCGTGCCTTACCGCtt                                                                                                                    >  1:794835/1‑143 (MQ=255)                           gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTAGAACCTCGGAATGCCGGAATTAGAATCAGGTGCCTTACCGCTTGGCGATAc                                                                                                            >  2:740272/1‑143 (MQ=255)                               cAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCa                                                                                                        <  1:139611/143‑1 (MQ=255)                                 aCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAc                                                                                                      >  1:1000050/1‑143 (MQ=255)                                    tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGACTGCCGGAATTAGAATCCGGTGCCTTACCGCCTGGCGATACCCCACCTgg                                                                                                   >  1:1127888/1‑143 (MQ=255)                                    tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                   >  1:1127882/1‑143 (MQ=255)                                    tCGCAGTCGACAGGGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGAGTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                   <  2:588349/143‑1 (MQ=25)                                         ttCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                              <  1:979795/143‑1 (MQ=255)                                            gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACtt                                                                                           <  2:878396/143‑1 (MQ=255)                                               aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                                                                                        >  2:926598/1‑143 (MQ=255)                                                    atgaaCGCAGAAAAGCAAAAAGCTCGCCGAGTCGAGCTTTTTTAATGTGGATGAGCAACGATGGTTCGAACATGTGTATGCCGGGATTAGCATCCGGTGCCTTACCGCTTGGGGAGACCCGAACCGGGGGCACGTTCAAgggc                                                                                   >  2:808622/1‑141 (MQ=255)                                                             gagaaGCAAAAAGCTCGCGGAAGGGAGCTGTTTGAATGTGGCGGGGGTACGAGGATTCGAACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg                                                                          <  2:91291/140‑1 (MQ=21)                                                                aaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAAt                                                                       <  1:885608/143‑1 (MQ=255)                                                                   cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa                                                                    <  1:181731/143‑1 (MQ=255)                                                                    aaagaGCTTGGCGATGCGAGCTTTTGTAGTGGGGCTGGGGTACGAGTATTCGAACCTCGGAATGGCGTAATTAGAATCCGGTGCCTTACCGCTTGGCTATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt                                                                   <  2:17010/143‑1 (MQ=21)                                                                       aaGCTCGCCGAAGCGAGCTTTTTTAATGTGGTTGCGATACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGGTAGCCCAACGAGGTGCAGTTACAAGGGGAGCGGCTTGAGTAAATTgg                                                                >  2:1116706/1‑143 (MQ=21)                                                                        aggtCGCCACAGCGAGCTTTTTTAATGGGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc                                                               <  2:1072966/140‑1 (MQ=25)                                                                          ctaggcGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTg                                                             <  1:1060110/138‑1 (MQ=255)                                                                             gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGCCTAAATTGGCTgggg                                                          >  1:198800/1‑143 (MQ=255)                                                                              ccGAAGCGAGCTTTTTTAGTGTGGCTGGGGTACGAGGATTCGAGCCTCGGAATGCCGGAATGAGAATCCGGTGCCTTACCGCTTGGCGATACCCAAACTGGGTGCACTTACAAGGCAAGCGGCTTCACTACATCGGCCGCGGt                                                         >  1:795816/1‑143 (MQ=18)                                                                                 aaGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACg                                                      <  2:673165/143‑1 (MQ=255)                                                                                  ggCGAGCTTTTCTAACGTGGCGGGGGTACGAGGAGTCGAACCTCGGAATGCCGGACTTAGAATCCGGTGCGTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                     <  1:618895/142‑1 (MQ=25)                                                                                     gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTGCGCggc                                                  >  1:181136/1‑142 (MQ=255)                                                                                       gCTTGTTTAATGTGGCTGGGGTACGAGGATGCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAtt                                                <  1:795838/143‑1 (MQ=255)                                                                                         ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATATATTGGCTGGGGTACGAGGAttgg                                              >  1:29729/1‑141 (MQ=255)                                                                                          tttttAACGTGGCTGGGGTACGCGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGa                                             <  1:444644/143‑1 (MQ=255)                                                                                           ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                            >  1:404541/1‑143 (MQ=255)                                                                                                       ggggcTACGAGGATTCCAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                <  1:497879/142‑1 (MQ=255)                                                                                                               gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAGTGCCGGAATCAg                        >  1:6281/1‑143 (MQ=255)                                                                                                                      cGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                 <  1:232626/143‑1 (MQ=255)                                                                                                                         aCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc              <  1:1116706/143‑1 (MQ=255)                                                                                                                            tCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACACGGTATGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGGATGCCGGAATCAGCATCCgggtccgt           >  2:1072990/1‑137 (MQ=37)                                                                                                                                     cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGACCCTCGGCGTGACGGGATCAGAATCCGGTGCCTTACCGCTTgg  >  1:769297/1‑143 (MQ=37)                                                                                                                                     cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg  >  1:1127314/1‑143 (MQ=255)                                                                                                                                           |                                                                                                                                         TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696332‑696606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑