| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 97.8% | 121.9 / NA | 47 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (19/26); minor base C (0/1); total (19/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
CAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760346‑760621 | cAAGGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCg < 1:262258/143‑1 (MQ=255)cAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCg > 2:757970/1‑143 (MQ=255) tGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGGAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCgg < 1:279697/143‑1 (MQ=255) gaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaac > 1:633729/1‑142 (MQ=255) gaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaa < 1:934491/143‑1 (MQ=255) gaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaa > 1:579351/1‑143 (MQ=255) gCAGGGGCTGGCGAATCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCTGTCTCGCGTTGCCACGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACg < 2:404358/143‑1 (MQ=255) gCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGTCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACg < 1:571273/143‑1 (MQ=255) gCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGACACCCTCGCTCCCGCCACGCTGGt > 2:250289/1‑143 (MQ=255) gAAACGCAACAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtg < 2:119804/143‑1 (MQ=255) aaaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtga < 1:1134186/143‑1 (MQ=255) cGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgatga < 1:972977/143‑1 (MQ=255) cATCAGCACGGTGCCGGAAGCAGTTGAAATGAAGTCTCGCGTTGCCAAGATTTATGGCGATCGCGAGGCGATGGATGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGGAAACCTAGCTTACGTCAAGCTGGTTGATGAAg > 1:501221/1‑143 (MQ=255) tCAGCACGGTTCCGGAAGCAGTTGAAATGCAGTCTCGCATTGCCAGCATTTATGGCGATAGCCAGGCGATGCCTGCCGCTGAGAAACTGTTCGACTGGAGCGGTGCGGAACAACTCGCTTACGCTACGATGGTagctgagcgc > 2:1028133/1‑139 (MQ=255) cACGGTGCCGGAAGCAGTTGCAATGCAGTCTCACGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGCTGATGAAGGCATTc > 2:595740/1‑143 (MQ=255) cgCCCGAAGCAGTTGAAATGCAGGCTCGCGGTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAACCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGt‑t < 1:229636/142‑1 (MQ=255) ccGGAAGCAGTTGAAAGGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCg < 2:1127264/143‑1 (MQ=255) cGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGGAACTGTTGGGCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGC‑TCGc > 2:43807/1‑143 (MQ=255) cGGAAGCAGGTGAAATGCAGGCGCGCGTTGCCAAGAGTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGc < 1:387940/143‑1 (MQ=255) aGTTGAAATGCAGTCTCGCGTTGCCAAGATCTATGGCGTGCGTGAGGTGGTGTCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCgg > 1:1083920/1‑143 (MQ=255) ttGAAATGCAGTCGCGGGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCGGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGt < 2:57367/143‑1 (MQ=255) ttGAAATGCAGTCGCGCGGTGCCAAGATTTATGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAAGCGGTTCGACGGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGt < 2:925090/143‑1 (MQ=255) acgtGCGGTCTCGCCGTGCCAGGGTTTACGGCCATCGCCAGGCGGTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTACCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGaa < 2:243523/140‑1 (MQ=255) aagtGCTGTCTCGCGTTGCCAATATTTATGGCGATCGCGAGGCGCTGGCTGCCGGTGAGAACCTGTGCGACTGGGGCGGTGCGGGAACCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGaa < 2:30641/140‑1 (MQ=255) tctcGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGGAAACCTCGCTTACGCCACGCTGGTTGATGGAGGCATTCCGGT‑TCGCCTGTCGGGTGAGGACTCCgg > 1:991628/1‑143 (MQ=255) tcGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTc > 2:643886/1‑143 (MQ=255) gcgTTGCCAAGATTTATGACGATCGCCAGGCGATGGCTGCCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCATACGCCACGCTGGGTGATGGAGGCAGCCCGGT‑TCCCCTGCCGGGTGGAGACCCCgggagc > 2:613254/1‑139 (MQ=255) gTTGCCAAGATTTGTTGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCgg < 2:1000389/143‑1 (MQ=255) cAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGGAGACTCCGGGCGCGGTACct > 2:969730/1‑143 (MQ=255) aGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGC‑TCGCCTGCCGGGTGAAGACTCCGGTCGCGGTACctgc > 2:518111/1‑141 (MQ=255) tttATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTTCGGAAACCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttc < 2:582087/143‑1 (MQ=255) tttATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCA‑TCAGTTAAAGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttc > 2:802478/1‑143 (MQ=255) tGGCGATCGCCCGGGGATGGCTGCCGGTGAGATACTGTTCGACTGGAGCGGTGCGGCGCTTCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGTCTCCGTTCGCGGTACCTTCTTCCAcc < 2:131665/143‑1 (MQ=255) tGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAcc < 1:395392/143‑1 (MQ=255) gCCAGGCGATGGCTGGCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgcg < 2:1124651/143‑1 (MQ=255) gCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAATCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGCCTGACGACGCCTGTCGTGCTACCTTCTTCCACCGCCAcgcg > 2:500216/1‑143 (MQ=255) gCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgcg < 2:1023267/143‑1 (MQ=255) gCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGAt < 1:757970/143‑1 (MQ=255) tGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCcac > 1:1106087/1‑143 (MQ=255) ggCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCCGTGATCcaca < 2:579351/143‑1 (MQ=255) cTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAc < 1:643886/143‑1 (MQ=255) cTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAc < 1:629301/143‑1 (MQ=255) cGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCCTTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCGTCTTCCACCGCCCCGAGGTGATCCACAACCAGt > 1:462138/1‑143 (MQ=255) tGATAAACTGTTCGACTTGCGCGGTGTGGAAATCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCTGT‑TCGCCTGTCGGCTGATGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTa < 2:306997/143‑1 (MQ=255) gtgcGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCa < 1:161514/140‑1 (MQ=255) tCGACGGGAGCCGTGCCGAAATCCTCGCTCACGCCACGCTGGTGGATGACGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACt < 2:471555/143‑1 (MQ=255) gACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCCCAACCAGTCTAACGGTCCCACtcc > 2:329677/1‑141 (MQ=255) | CAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760346‑760621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |