Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	46 (1.150)	41/264	NT	100%	intergenic (‑271/‑267)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	39 (0.980)	31/264	NT	100%	intergenic (‑274/‑264)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255                                                                                                                                              |                                                                                                                                               aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGCTCTCATATCAGGGACTTGTTCGCACCTTCCa                                                                                                                                               >  2:178544/1‑143 (MQ=11)   gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATa                                                                                                                                             >  1:534705/1‑143 (MQ=32)   gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCATGTTCTATGGCTCCAGATGACAAACATGATCACAAATCAGGGACTTGGTCGCACCTTCCaaa                                                                                                                                             >  2:500297/1‑141 (MQ=255)    aaaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCAtta                                                                                                                                            <  1:260631/143‑3 (MQ=14)           ctTTTGTGGGCTGACGGCGCTTACTGCTGAATCCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                     <  2:155460/143‑1 (MQ=255)           ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                     <  2:521008/143‑1 (MQ=38)             tttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGc                                                                                                                                   <  1:434155/143‑1 (MQ=40)                    gcTGTCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGa                                                                                                                            <  1:356693/142‑1 (MQ=255)                      tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAAc                                                                                                                          <  2:265692/143‑1 (MQ=255)                           gcgcTGACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGata                                                                                                                     >  1:651327/1‑143 (MQ=255)                                   tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGCACAGATATTGCGGaa                                                                                                             >  1:302987/1‑143 (MQ=255)                                    gctgAATTCAGCGTCGGCGAAGGTAAGTTGATGACTGATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAAc                                                                                                            <  1:158447/143‑1 (MQ=255)                                     ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCGCATATCAGGCACTTGCTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGAAATCGCGGACCg                                                                                                           >  2:313026/1‑143 (MQ=17)                                                     cGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGaa                                                                                           >  1:455244/1‑143 (MQ=255)                                                        aGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACCAACATGATATCATATCAGGGACTTGTTCGCACCATCCATAACGCTGTACCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCa                                                                                        >  2:390113/1‑143 (MQ=255)                                                              gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGa                                                                                  >  1:190096/1‑143 (MQ=255)                                                               ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGAt                                                                                 >  1:67361/1‑143 (MQ=255)                                                                       tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAg                                                                         >  1:511974/1‑143 (MQ=255)                                                                       tCATGATCGACCCTGTTCTATAGCTCCATATGACAAACATTATCTCATATCAGGGACTTGTTCGCCCCCTCCATAACGCTGTATCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAg                                                                         <  1:343352/143‑1 (MQ=37)                                                                                  ccTGTTCTATGGCTCCAGATGACAAACATGATATCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGTGGAACGACAAAGAGAAACAGAACCGGATCGCTGCGTCGAGCTTtcctccca                                                              >  1:633245/1‑141 (MQ=25)                                                                                        cTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCACGAACACAACAGCAACGAGAACAGGTATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTCTgccac                                                        >  2:767259/1‑139 (MQ=21)                                                                                              cTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATAGAGCTTTCATCCTATGAAATTAATTg                                                  >  2:97944/1‑143 (MQ=255)                                                                                               tCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGTTTTCATCCTATGAAGTTAATTGc                                                 >  2:784094/1‑143 (MQ=255)                                                                                                   gATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTACCCACCAGAACAGATATTGCGGAACGACACAGAGCAACAGAACCAGATTGATGCATTGAGCTTTAATGCGAAGTGATTAACTGCTGtt                                             >  1:324177/1‑143 (MQ=37)                                                                                                       gcAAACATGATCTCATATCAGGGACTCGTTCGCACCTTCCATAACGCTGTAGCCAGCAGAACAGATATTGCGGAACGACAAAGAGAAACACAACCAGATTGATGCATTGAGCTTTCACCCTATGAAATTAATTACTCTTaaaa                                         >  2:751760/2‑143 (MQ=38)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGCAACAGAACCAGATTGATGCATTGAGCTTTCGTCCTATGAACTTAATTGCTGTTAACAGc                                       >  1:167115/1‑143 (MQ=255)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                       <  1:764983/143‑1 (MQ=255)                                                                                                            cATGATCTCATATCAGGGACTTGTGCGCACCTTCCAGAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                    <  1:341988/143‑1 (MQ=255)                                                                                                              tGATGTCATATCAGGGACGTGTTCGCGCCTTCCATAACGCGGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAGCCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTgg                                  <  1:602718/143‑1 (MQ=255)                                                                                                                   tcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGGTATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACa                             >  1:218386/1‑143 (MQ=255)                                                                                                                   tcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACa                             >  1:138920/1‑143 (MQ=255)                                                                                                                             gACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAccc                   >  1:494178/1‑143 (MQ=255)                                                                                                                                    tCGCACCTTCCATAACGCTGTAGCCACCAGAACAGAGATTGCGGAACGACAAAGAGAAACTGCACCATATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTc            <  1:180435/143‑1 (MQ=255)                                                                                                                                     cGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTcc           >  1:368035/1‑143 (MQ=255)                                                                                                                                     cGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTcc           >  1:199706/1‑143 (MQ=255)                                                                                                                                         ccTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAc       >  1:622619/1‑143 (MQ=255)                                                                                                                                              cATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGa  <  2:511974/143‑1 (MQ=255)                                                                                                                                              cATAACGCTGGAGCCACGAGAACAGAGATTGCGGAACGACAAAGAGAAACAGAACCAGATTGCTGCATTGAGCTTTCATCCTATGAAATGAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCgta  <  2:316982/143‑3 (MQ=255)                                                                                                                                              |                                                                                                                                               AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.