Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	118.5 / NA	40	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/16);  total (24/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754306‑754584                                                                                                                                             |                                                                                                                                           gttCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACTCCTTTTGAACCGAGAGTACGGACATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAgggtgggt                                                                                                                                          <  2:435842/142‑1 (MQ=255) aTTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAgggtgggt                                                                                                                                          <  2:435839/143‑1 (MQ=255)        ggATGCGGTTGAAGTGAAGCCTGGGGCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtt                                                                                                                                   <  2:299195/143‑1 (MQ=255)          cggCGGGTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATACCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                                 <  1:220260/141‑1 (MQ=255)          aTGCGGTTGAAGTGGAGTCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACTTCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                                 <  2:268884/143‑1 (MQ=255)                           ccTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTCTCAGCCATTTAAgg                                                                                                                >  2:572399/1‑143 (MQ=255)                           ccTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAGTTCAgg                                                                                                                >  1:717427/1‑143 (MQ=255)                                  cAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                                         >  1:383651/1‑143 (MQ=255)                                  cAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                                         <  2:621986/143‑1 (MQ=255)                                   aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTGCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCGTTTCAGGTCTCCTTa                                                                                                        >  2:391577/1‑143 (MQ=255)                                    aaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAg                                                                                                       >  1:675329/1‑143 (MQ=255)                                      ggTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACCTCCAGTTCACGAGCTGTCTCCTCGTTGAGGGCGGGTCTTGCCTTTGTACCAGGCCTTTGAGGGAGCCTTAgtc                                                                                                     >  2:725625/1‑141 (MQ=255)                                      ggTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATGCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCGTTTGTATCAGCCATTTCACGTCCACTTAtcg                                                                                                     >  2:562788/1‑143 (MQ=255)                                        tGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGcc                                                                                                   <  1:95221/143‑1 (MQ=255)                                             acgccTTTCGAACCGAGAGTACGCACGTCAACAACAGCCTGCCCCACCGTGCCTTTCAGCACATCCAGTTCGACACCTGTATCCCCGTTGAGGGTGGGTTTTGCTTCTGTATCAGCCATTTAAGGGCTCCTTAGCGCCTTAtt                                                                                              <  2:115646/140‑1 (MQ=255)                                             acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                              <  1:77089/143‑1 (MQ=255)                                                   tttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                                                        >  1:153824/1‑143 (MQ=255)                                                           gagaGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCGGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCg                                                                                <  2:331085/143‑1 (MQ=255)                                                                   ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGGGGGTTTTGCTTTTTTATCAGCCATTTAAGGTCTCCTTAGCGCCAAATTGCGTAAGACTGACGGAACTaaa                                                                        >  1:684745/1‑143 (MQ=255)                                                                        tCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTg                                                                   <  2:736435/143‑1 (MQ=255)                                                                         cAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGc                                                                  <  1:762409/143‑1 (MQ=255)                                                                          aaTAACATCTTGACCCAGCGTGCCTTTCAGCACCTCCAGTTCAACCGCTGTATCCCCGTTGAGGGCGGGTTTCGCTCTTGTATCAGCCGTTTAAGGTCTCCTTAGCGCCTTCTTGCGTACGACCGCCCGAACTCACATCTGcc                                                                 >  2:55336/1‑143 (MQ=255)                                                                          aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGAGTGCCGGAACTTAAATTTGcc                                                                 >  1:453678/1‑143 (MQ=255)                                                                            tAACATCTCGCCCCAGCGTGTCTTTCACCACACTCAGTTCCACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGCATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCtt                                                               <  2:422161/143‑1 (MQ=255)                                                                             aaCAGCTTTACCCAGAGTGCCTTGCAGCACACCCAGCTCAACAGCTGTAGCCGCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTc                                                              <  1:481866/143‑1 (MQ=255)                                                                                  cTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGGCGCCTTAGCGCCTTTTTGCGTAAGACTGCCGGAGATTAAGGTTTCCTTCGcgca                                                         >  1:544206/1‑143 (MQ=255)                                                                                   ttGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGGGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACAt                                                        >  2:653575/1‑143 (MQ=255)                                                                                   ttGACACAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGcacgt                                                        >  2:136141/1‑141 (MQ=255)                                                                                      aCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCaa                                                     >  1:770820/1‑143 (MQ=255)                                                                                              tGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCtt                                             >  1:373072/1‑143 (MQ=255)                                                                                               gCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGccgt                                            >  1:262904/1‑140 (MQ=255)                                                                                                    tCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAACTTTGCCTTCGCACATCAACCCGGCTTTACCCg                                       >  2:516601/1‑143 (MQ=255)                                                                                                           aTCCAGTTCAACAGGTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCGCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTa                                <  1:682568/143‑1 (MQ=255)                                                                                                            tCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACCTCAACCTGCCTTTACCCGTTTTTTac                               >  2:373651/1‑142 (MQ=255)                                                                                                             ccAGTTCAACAGCTGTATCCCCGTTGAGGGTGGATATTGATTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTCTTTAtt                              >  2:770071/1‑143 (MQ=255)                                                                                                               aGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCATTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACATGGCTTTACCCGTTTTTTATTTg                            >  1:28137/1‑143 (MQ=255)                                                                                                                    aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcg                       <  1:747139/143‑1 (MQ=255)                                                                                                                             gtCCCGGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAGCGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCATCCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt              <  2:764933/142‑1 (MQ=255)                                                                                                                               ccccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGa            >  1:174498/1‑143 (MQ=255)                                                                                                                                      gAGGGTGGCTTCGGCTTTTGTCTCATCCATTTAAGCTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg     <  1:559510/143‑1 (MQ=255)                                                                                                                                       aGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTCCCCGTTTTCTATTTGGCTCGCCGCTCTGTGAAAGAgggg    >  1:368642/1‑143 (MQ=255)                                                                                                                                         ggTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaa  >  1:732421/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                           ATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754306‑754584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑