| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | 100% | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 100.0% | 101.0 / ‑6.7 | 43 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (24/17); minor base C (1/1); total (25/18) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCC > NC_000913/760348‑760616 | gaGTTGAGAGGAAGCGCCTGCAGGAGCGGGAGAAACGGATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGt < 2:468966/142‑1 (MQ=255) gAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAcc > 1:361040/1‑143 (MQ=255) gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGACcgcg > 1:393809/1‑143 (MQ=255) gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGACCTCg > 1:688419/1‑143 (MQ=255) cAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCCGGCGATGGCTGCCGGTGAGAAACTGTTAGACTGGAGCGGTGCGGAAGACCTCGCTTACGc > 2:594501/1‑143 (MQ=255) aGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTGCGGAATACCTCGCTTACGcc > 1:153139/1‑143 (MQ=255) aGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGAGCGGCAGGCGATGGCTGCCGGGGAGACACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGcc > 1:545312/1‑143 (MQ=255) aGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACg < 2:618446/143‑1 (MQ=255) gAAACGCATCAGCACGGTGCCCGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGCTtg > 1:317337/1‑143 (MQ=255) agaCGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGCCTCGAATCGCTGCACTTCATGGCGTTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtga < 1:573166/141‑1 (MQ=255) aaaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtga < 1:770356/143‑1 (MQ=255) aaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGGCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgat < 1:636697/143‑1 (MQ=255) cATCAGGACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAg < 1:223056/143‑1 (MQ=255) aTCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCAAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGACACACTCACTTAAGCTGCGCTGATTGATGaaag > 2:3864/1‑141 (MQ=255) gCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAACACCTCGCTTACGCCTCGCTGGTTGATGAAGGCAtt > 1:347912/1‑143 (MQ=255) gCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGACGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTAGGCCACGCTGGTTGATGAAGGCAtt > 1:498539/1‑143 (MQ=255) cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGCACACCTCGCTTACGCCACGCTGGCGGACGACGGCATTc > 2:400567/1‑143 (MQ=255) cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCCTTc > 1:524894/1‑143 (MQ=255) cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGAGAGAAACTGTTCGACTGGAGCGGTGAGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCAtgc > 2:604804/1‑141 (MQ=255) cgcaaGCCGTTTAAATGCAGTCTCGCGTTGCTCTGATTTCTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGAGTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc < 2:211021/140‑1 (MQ=255) gAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGATAAACTGTTCGACTGAAGCGGTGCGGAAAACCTCGCTTACGACACGCTGGTTGATGAAGGAATTCCGGTTCGCCt > 2:535383/1‑143 (MQ=255) ttGAACTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGt < 2:653222/143‑1 (MQ=255) aTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGa > 1:286021/1‑143 (MQ=255) tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGACGGCATTCCCGTTCGCCCGTCGCGtcacgcc > 1:364407/1‑137 (MQ=255) tcGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTc > 1:392193/1‑143 (MQ=255) gCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAc < 2:241264/143‑1 (MQ=255) ccAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAcc > 1:381816/1‑143 (MQ=255) cAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACct > 1:121291/1‑143 (MQ=255) aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGCAACTGTTCGACTGGAGCGGTGCGGACAACGTCGCTTACGCCCCGCTGGTTGATGAGGGCATTCCGGTTCGCCTGTCGGGTGAAGCCTCCGGGCGCGGTGCctt > 2:93502/1‑143 (MQ=255) cAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGGCTCCGGGCGCGGTACCTTCTTCCACCGCCACGCGGt > 2:303229/1‑143 (MQ=255) cggtGGCTGCCTGTGAGAAACTGTTCGACGGGAGCGGTGCGGAAACCCTCGCTTACGCCGCGCTGGTTGATGGAGGCATTCCGGTTCGCCTGTCGGGTGCAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATc < 2:180975/140‑1 (MQ=255) cgcgggCTGCCCGTGAGAAACTGTTTGACTGGCGCGGTGCGGACACCCTCGCTTACGCCACGCGGGTTGATGAGGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATc < 2:181237/139‑1 (MQ=255) cGATGGCTGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAGGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATc < 2:516234/143‑1 (MQ=255) aTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCCCCCGGTGATccg > 1:16156/1‑142 (MQ=255) tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTGCCTTCTTCCACCGCCACGCGGTGGTCCACAAcc > 1:93253/1‑143 (MQ=255) cccGGTGCGAAGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCa < 1:594501/142‑1 (MQ=255) ccGGTGAGAAACTGATCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACCCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACCACCAg > 2:285952/1‑143 (MQ=255) cGGTGAGAGACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTGACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGt < 2:6036/143‑1 (MQ=255) gtcgagaAGCTGTTCGGCTGGATCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTc < 2:361040/140‑1 (MQ=255) agaAACTGTACGACCGGAGCGGAGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAAc < 1:749722/143‑1 (MQ=255) gaAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACg > 1:449972/1‑143 (MQ=255) atttttCGACTGTAGTTGTGCTGAAATTCTTTCTTACGCCACTCTGGTTGATGATGGCATTCCTGTTCGCCTGTCGGGTGAAGTCTCCGGTCGCGGTATCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGtt < 2:619286/139‑1 (MQ=255) tGTTCGACTGGAGCGGTGCGGCAGACCTCGCTTACGCCACGCTGGTTGATGAAGGCAGTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTcc < 2:498539/143‑1 (MQ=255) | AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCC > NC_000913/760348‑760616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |