| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,113,546 | T→A | 14.0% | intergenic (+140/‑33) | opgH → / → yceK | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H/DUF1375 domain‑containing lipoprotein YceK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,113,546 | 0 | T | A | 14.0% | 87.4 / 6.8 | 38 | intergenic (+140/‑33) | opgH/yceK | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H/DUF1375 domain‑containing lipoprotein YceK |
| Reads supporting (aligned to +/- strand): ref base T (13/18); new base A (3/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
AAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATT > NC_000913/1113407‑1113678 | acacctAGTTGCCGGATGCGCTACGCTTAGCAGGCCTACATCGTTCCTGCAATTTATTGCTTTAGCCAGCGGTTGGGGGGCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATg < 2:236579/138‑1 (MQ=21) cGTAGTTGCCTGATGAGCTAGGCTTATCAGGTCTACATCGGTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCg < 2:116511/143‑1 (MQ=255) cGTAGTTGCCCGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCg < 1:456934/143‑1 (MQ=255) tAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTC‑‑AGGCCGGt > 2:461576/1‑143 (MQ=255) cgCCTGATGCGCCACGCTTATCAGGCCTACATCGTTCCTGCACTTTAGTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTAttt < 1:706879/142‑1 (MQ=255) ccTGATGCACTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATA‑‑ACGCCGGTAttttt < 1:181330/143‑1 (MQ=37) gATGCGCCACGCTTATCAGGCATACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTa < 1:354580/143‑1 (MQ=255) aCGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAagag > 1:438178/1‑143 (MQ=255) ggCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTCTTTTTTAACGAAAGAGGTATCAAATg > 2:420097/1‑143 (MQ=255) ggCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGAT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCACATg > 1:788075/1‑143 (MQ=255) gCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGc > 2:429226/1‑143 (MQ=255) gCCTACATCGGTCCTGCAATTTAGTGATCTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGc < 1:55576/143‑1 (MQ=255) tCGTTCCTGCAATGCATGGATGTTGCAAGACTTTGTGGGTCGGACAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAAt < 1:299952/143‑1 (MQ=39) tCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGTCTTCGCTACGAACCAAT‑AACAGA‑CGGAGTAAGAGGTCTGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtgtg < 1:577429/143‑1 (MQ=16) tCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGAGAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtgtg < 1:602466/143‑1 (MQ=255) cttcAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggt < 2:46399/140‑1 (MQ=255) ttgttGATTTTCCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatc < 2:706596/140‑1 (MQ=255) ttGATCTTGCAAGACTTTGGGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATg < 1:289532/143‑1 (MQ=255) ttttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGa > 2:590723/1‑143 (MQ=255) gCAAGACGTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTGTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCtt < 2:276413/143‑1 (MQ=255) aaGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTGGc > 1:174186/1‑143 (MQ=255) aaGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGGG‑‑ATGCCGGTATTTTTTTATGAAGGAGGTAACAAAACCGTTAATTTGAGGTGGGCATCATGGTGACCTTGc > 1:570784/1‑143 (MQ=37) aCTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTCTTTGTGGTGAGCATCATGGTGACCTTGCTGa > 1:710960/1‑143 (MQ=255) tgGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCt < 1:634441/143‑1 (MQ=255) ggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgt < 1:123766/143‑1 (MQ=255) tCGGATAAGGCGTGCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTgg < 1:324996/143‑1 (MQ=255) gCGTTCACGCCGCATCCGGCAAGAGCAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTGACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattat < 2:175477/143‑1 (MQ=255) gccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCa < 1:590723/143‑1 (MQ=255) cATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCCAATGCGTCTAATTGTGGTGAGCATCCTGGTGACCTTGCTGAGTGGCTCTGGCAGCATTCTTAGTCGCACtat > 2:326452/1‑143 (MQ=255) aTCCGGCAAAGACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCCTGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACtatg > 2:137024/1‑142 (MQ=255) tCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATAc > 2:367259/1‑143 (MQ=255) tCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATAc < 2:501100/143‑1 (MQ=255) cGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGCTGACCTTGCTGGGTGGCTGTGGCAGCATTATTATTCGCACTATACCg > 1:469921/1‑143 (MQ=255) aacGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggc > 1:261564/1‑143 (MQ=255) tcgtaaaaaaaTACCGGCATA‑‑ACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGCGGCAGCATTATTAGTCGCACTATACCGGGGCCGGGGCACGGc > 2:259499/7‑143 (MQ=38) aaaaaaaTACCGGCATA‑‑ACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTCTAATTGTGGTGAGCATCATGGTCACCTTGCTGAGTGTCTGTGGCAGCACTATTAGTCGCACTATACCGGGGCGGCGGCATggtcacc > 2:486109/3‑138 (MQ=255) aTAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAAcc > 1:774238/1‑143 (MQ=255) aTACCGGCATA‑‑ACGCCGGTATTTTTATACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCGAtatt > 2:492415/1‑143 (MQ=255) | AAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTT‑‑ATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATT > NC_000913/1113407‑1113678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |