Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 124,241 C→T 100% Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913124,2410CT100.0% 112.9 / NA 43Q409* (CAG→TAG) aceEpyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/27);  total (16/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCT  >  NC_000913/124102‑124371
                                                                                                                                           |                                                                                                                                  
tggtggTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAgg                                                                                                                                 <  1:155859/143‑1 (MQ=255)
        aCGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaa                                                                                                                         >  2:28989/1‑143 (MQ=255)
         cGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaaa                                                                                                                        >  1:647227/1‑143 (MQ=255)
         cGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaaa                                                                                                                        <  1:263010/143‑1 (MQ=255)
         cGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaaa                                                                                                                        <  1:69519/143‑1 (MQ=255)
          gATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTCAGAAAAt                                                                                                                       >  1:275276/1‑143 (MQ=255)
                aggaaAAGCGAAGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGGACAGCGGCCGGAATCCCTGCTGATCCCATTAAAGGTTACGGCATGGGCGACGCGGCTGCAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt                                                                                                                 <  2:133518/141‑1 (MQ=255)
                   aaaaTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGa                                                                                                              >  2:414832/1‑143 (MQ=255)
                         gaCGCTGGATTCGAGAAAGCGGAGGAACCCAAAGGCAAAGCGACAGTGGTCCTTGCTCATACCATTAGAGGTTACGGCATGGGCGACGCGGCGGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtgt                                                                                                        <  2:731418/142‑1 (MQ=255)
                           ggCTTCAGTCAAGCAAGCGCAGGAAACGAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGc                                                                                                      <  2:695640/142‑1 (MQ=255)
                                 aTTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTGAGAAACTGAACATGGACGGTGTGCGTCatg                                                                                                >  1:675382/1‑142 (MQ=255)
                                  ttCAAGAAAGCGCAGGAAAACAAAGGCAAAGAGACAGTAATCCTTGGTCATACCATGAGAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCatat                                                                                               <  2:67952/143‑1 (MQ=255)
                                      aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgc                                                                                           <  2:402786/143‑1 (MQ=255)
                                      aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGAAGGTGTGCGTCatccacga                                                                                           >  1:100407/1‑137 (MQ=255)
                                                 gAAACGAAAGGCAAGGCGTCAGTGATCCTTGCTCAGAGCAGTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCaa                                                                                <  2:234871/143‑1 (MQ=255)
                                                 gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATCAAAGGTTCCGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCaa                                                                                <  2:104930/143‑1 (MQ=255)
                                                      cAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAGGGTCACAGACTATGGCACCTCTCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGc                                                                           <  1:781262/143‑1 (MQ=255)
                                                      cAAAGGCAAAGCGACAGTAATCCTTGCTCATACCAATAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCACATCCGCGACCGTTTCAATGTGc                                                                           >  2:586014/1‑143 (MQ=255)
                                                              aaGCGGCAGGAATCCTGGCTCATACAGTTCAAGTGTGCGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCt                                                                   <  2:378059/143‑1 (MQ=255)
                                                              aaGCGACAGTAATCCTGGCTCAGACCATTAAAGGTTACGGGATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTGAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCt                                                                   <  2:609737/143‑1 (MQ=255)
                                                                  gACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATg                                                               <  2:23741/143‑1 (MQ=255)
                                                                    cAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCa                                                             <  1:663347/143‑1 (MQ=255)
                                                                    cAGTAATCCTTGCTCATACCAACACAGGTTACTGTATGGGCGACGCGGCTTAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCa                                                             <  1:570962/143‑1 (MQ=255)
                                                                        agtCCTTGCTCATACCATTATGTGTTACGGCATGGGGTACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAGTGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGata                                                         <  2:661799/141‑1 (MQ=255)
                                                                             ttGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaa                                                    >  1:275871/1‑143 (MQ=255)
                                                                                 tCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAAc                                                >  1:294004/1‑143 (MQ=255)
                                                                                         ttAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTAc                                        >  1:588349/1‑143 (MQ=255)
                                                                                         ttAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTAc                                        >  1:93515/1‑143 (MQ=255)
                                                                                          tAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGAGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATCCAGATATCGCAAAAGTGCCGTACa                                       >  1:462772/1‑143 (MQ=255)
                                                                                            aaGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATc                                     <  2:621389/143‑1 (MQ=255)
                                                                                            aaGGTGACGGCATGGGCGACGTGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGCGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATc                                     <  2:416757/143‑1 (MQ=255)
                                                                                             aGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCa                                    <  1:116021/143‑1 (MQ=255)
                                                                                              ggTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCAc                                   <  1:28989/143‑1 (MQ=255)
                                                                                              ggTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGGTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCACTGTGCCGGTGTCTGATGCAGATATCGAACAACTGCCGTACATCAc                                   >  2:587751/1‑143 (MQ=255)
                                                                                                               cgcgGCCGAAGGTAAAAACATCGCCCACTAGGTTAGCAAAATAAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTg                  <  1:144405/143‑1 (MQ=255)
                                                                                                                   ggggaccgTAAAAACAACGCGCACTAGGTTAAGACAATGAACATGGCCGGTGTGCGTCATATCCGCGACCGGTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAaga              <  1:594926/136‑1 (MQ=255)
                                                                                                                   gCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAaga              <  2:735837/143‑1 (MQ=255)
                                                                                                                    cTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAagag             <  1:510564/143‑1 (MQ=255)
                                                                                                                     tGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGATTCCATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCAGGAACGTCCTGAAGAGc            >  2:662025/1‑143 (MQ=255)
                                                                                                                         tgTAAAAACATGGCGCACTAGGGTAAGAAAATGAACATGGACGGTGTGCGTCAAATCCGCGACCGGGGCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATAc        <  1:24353/142‑1 (MQ=255)
                                                                                                                          gTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGCTCCAATGTGCCGGTGCCTAATGCGGAGATCGAAAAACCGCCGTGCATCGCCTTCCCGGAAGGGTCCGACGAGCacgcc       >  2:489600/1‑139 (MQ=255)
                                                                                                                            aaaaaCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTa     >  1:23359/1‑143 (MQ=255)
                                                                                                                               cccATCGCGCCCTAGGTTAAGCAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCt  <  1:168039/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                  
TGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCT  >  NC_000913/124102‑124371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑