Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,226,835 C→A 11.8% T79K (ACA→AAA)  ycgJ → PF05666 family protein YcgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,226,8350CA11.8% 104.9 / 5.8 48T79K (ACA→AAA) ycgJPF05666 family protein YcgJ
Reads supporting (aligned to +/- strand):  ref base C (24/18);  new base A (3/3);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

CGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGT  >  NC_000913/1226697‑1226976
                                                                                                                                          |                                                                                                                                             
cGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTc                                                                                                                                           >  2:340587/1‑143 (MQ=255)
cGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTc                                                                                                                                           >  1:38709/1‑143 (MQ=255)
   cgagcggccTGGTTTGCGACCCGTATAGTTGCGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCt                                                                                                                                        <  1:250276/140‑1 (MQ=255)
    gTGCGGCGGTGTTGGCGACGCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtg                                                                                                                                       <  2:106576/143‑2 (MQ=255)
     cgcggcgTTGTGGGCGACCCGTATATTTGGGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtgg                                                                                                                                      <  1:528764/142‑3 (MQ=255)
         gcgTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTTGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCGCAGCGAATTTACATTCGCCAAAGgt                                                                                                                                  >  2:72009/1‑143 (MQ=255)
            ttgttTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTa                                                                                                                               <  2:20265/143‑1 (MQ=255)
            ttgttTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTa                                                                                                                               >  1:483755/1‑143 (MQ=255)
                    gACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCgc                                                                                                                       >  1:271992/1‑142 (MQ=255)
                     aCCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAACACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGAt                                                                                                                      >  2:129588/1‑143 (MQ=255)
                       cgggttatTGGTGTGAACTGGGATGGGCTTGCCCCAGAGTTGACGCGGAAATATCTCGGGGAAAAAGCCTCTGAAAACTTACATTCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGt                                                                                                                    <  2:348484/138‑1 (MQ=255)
                       cacgatatTTGTGGGAACTCAGATGGCATGTCCCCAGTGTCAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGt                                                                                                                    <  2:362447/139‑1 (MQ=255)
                         gTATATTTGTGTGAACTCGGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTa                                                                                                                  >  1:292542/1‑143 (MQ=255)
                          ttgatctgtgtgAACCGAGGTGGCATTTCGCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTaa                                                                                                                 <  2:106721/137‑1 (MQ=255)
                                 gtgtgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAGAGcaaag                                                                                                          >  2:651857/1‑142 (MQ=255)
                                    tgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTa                                                                                                       >  1:498880/1‑143 (MQ=255)
                                         tCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCg                                                                                                  >  2:744195/1‑143 (MQ=255)
                                                  aTTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGCTGGTCAAGAAAAATCATGTCGTGATGCTCg                                                                                         >  2:711494/1‑143 (MQ=255)
                                                        ccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCTCTAACGGTGTACCTTACAACGTGAAAGAAACATTATGTAGTGATCATAGGTAttt                                                                                   >  2:762037/1‑143 (MQ=255)
                                                         cAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAtttt                                                                                  >  1:164760/1‑143 (MQ=255)
                                                          agagTTAACAGGTAAATATCTCGGCGGAAAATCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTAAACGAAAAATTATGTCGTGATGATCGTTATTTTg                                                                                 <  2:688421/143‑1 (MQ=255)
                                                                aaCAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTgg                                                                           >  1:620042/1‑143 (MQ=255)
                                                                       aaaTATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTAAATTCGATAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTaa                                                                    >  2:50255/1‑143 (MQ=255)
                                                                              tcGGCGAAAAAGGCGCTGAAGACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGt                                                             <  1:736053/143‑1 (MQ=255)
                                                                                 gCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGa                                                          <  2:402453/143‑1 (MQ=255)
                                                                                  cGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaa                                                         <  1:129588/143‑1 (MQ=255)
                                                                                   gAAAAAGCCGCTGAAAACTTACAAAATATACAAGTCTACGATCCCAGCGAACTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAGTTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaaa                                                        >  1:298080/1‑143 (MQ=255)
                                                                                         gCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatcaa                                                  <  1:372870/143‑1 (MQ=255)
                                                                                             cTGAAAACTTCCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAAtcaatcaa                                              <  2:292542/143‑1 (MQ=255)
                                                                                              tGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGTGTGGAAAAatcaatcaca                                             >  2:400416/1‑141 (MQ=255)
                                                                                                aaaaCTTACAATCATTACAAGGCTACGATGCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAacc                                           <  2:46887/143‑1 (MQ=255)
                                                                                                       aCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACaa                                    >  2:174773/1‑143 (MQ=255)
                                                                                                        ccttCATTCCAAGGCTACGTTCCCAGCGAATCTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACaaa                                   <  2:669294/140‑1 (MQ=255)
                                                                                                         aaTCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAg                                  >  2:355180/1‑143 (MQ=255)
                                                                                                          aTCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGa                                 >  2:748111/1‑143 (MQ=255)
                                                                                                           tCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTACAGCAAAATTATGTCGTGATGATCGTTATTTTGCTGTGGATGGTAAGCGGCGTGCAACAATCGATCAAACCACCACCACGAt                                >  2:265288/1‑143 (MQ=255)
                                                                                                               tACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGGTGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGtta                            >  2:273209/1‑143 (MQ=255)
                                                                                                               tACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGtta                            <  2:38709/143‑1 (MQ=255)
                                                                                                                           ccgttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGTAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgt                >  1:388550/6‑143 (MQ=255)
                                                                                                                           agcctaGCCAATTTACATTCGCTAACGGTGTATTTTGCGATGTAAGAGTAAAGTTATGTAGTAATGATCGTTATTTTGGTGTGGTTTGTAAGGGTAGTTGAAATATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgt                <  1:701368/138‑1 (MQ=255)
                                                                                                                           aTCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgt                >  1:535676/1‑143 (MQ=255)
                                                                                                                           aTCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgt                >  1:391031/1‑143 (MQ=255)
                                                                                                                            tCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGCGTGGAAAAATCAATGAACCCACCACAAAGATGTTATTTATGTGTCgtg               >  2:194036/1‑143 (MQ=255)
                                                                                                                               taGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTGATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAAGCACCACAAAGATGTTATTTATGTGTCttgcgt            >  2:43239/2‑143 (MQ=255)
                                                                                                                                  ggagtttACATTCGCCAACGGTGCATTTTGCCATGTTAAAGAAAAACTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGAt         <  1:418000/139‑1 (MQ=255)
                                                                                                                                  cGAATTTACATTCGCTAACGGTGTATTTTGCTATGTTAAAGAAAAATTATGTTGTGATGATTGTTATTTTGGTGTGGATGGTAAGAGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGAt         <  1:240138/143‑1 (MQ=255)
                                                                                                                                    aaTTTACATTCGTTAACGGTGTGTTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCTCCACAAAGATGTTATTTATGTGTCGTGAGTGATGt       <  2:661088/143‑1 (MQ=255)
                                                                                                                                         aCATTCGCTATCGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGt  <  2:176570/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                             
CGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGT  >  NC_000913/1226697‑1226976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑