Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,233,154 T→A 8.0% intergenic (‑124/+22) dsbB ← / ← nhaB protein thiol:quinone oxidoreductase DsbB/Na(+):H(+) antiporter NhaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,233,1540TA 8.0% 130.0 / 4.6 50intergenic (‑124/+22)dsbB/nhaBprotein thiol:quinone oxidoreductase DsbB/Na(+):H(+) antiporter NhaB
Reads supporting (aligned to +/- strand):  ref base T (22/24);  new base A (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

TTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCA  >  NC_000913/1233014‑1233292
                                                                                                                                            |                                                                                                                                          
ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCCCCTCCAGTCTGCATACATATGACGCAATTGAACAATTATCGAACAAAAAGGCACACCGGGCATTta                                                                                                                                          >  1:41219/1‑143 (MQ=255)
   aaaagTCGCAACATAATCACTCCCCGGCAATATGCATATAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTatat                                                                                                                                       <  2:493770/143‑1 (MQ=255)
   aaaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTatat                                                                                                                                       >  1:501221/1‑143 (MQ=255)
      aaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGcc                                                                                                                                    <  1:680338/143‑1 (MQ=255)
       aTCGCAACATATGCTTTTCTCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                                   <  2:464983/143‑1 (MQ=255)
          gCAACCTAATCCTTTCCCTGCAATATGCATAGAGTGCCCAGTTTAAACCAATTCATTCGCTGCGCCACCTGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGt                                                                                                                                <  1:548220/143‑1 (MQ=255)
           cAACATAATCATTTCCCTGCAGTAGGCATAGAGGGCGCAGTTTAAACCAATTCATTCGCGGCGCCACCAGGATGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTa                                                                                                                               <  1:674638/143‑1 (MQ=255)
           cAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATCTGCCCGGTa                                                                                                                               >  1:772622/1‑143 (MQ=255)
           cAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTa                                                                                                                               >  1:353631/1‑143 (MQ=255)
             aCATAATCATTGCCCTGCAATTTGCGTCGAGTGCGTAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTAGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtttg                                                                                                                             <  2:486988/143‑4 (MQ=255)
                ttaTCCTTTCCCTACAATATGCATAGAGTGCGCGGTTTAAACCAATTCATTCCCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttg                                                                                                                          <  1:309274/141‑1 (MQ=255)
                   tCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTa                                                                                                                       <  1:678298/143‑1 (MQ=255)
                   tCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTa                                                                                                                       <  2:513249/143‑1 (MQ=255)
                                  atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAt                                                                                                        <  2:778573/143‑1 (MQ=255)
                                   cggaTACAGTGCGCAGTTTAAGCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAGATAGGACGCAATTGTACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATc                                                                                                       <  2:501504/140‑1 (MQ=255)
                                      aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGACAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAtgcgg                                                                                                    >  2:266886/1‑139 (MQ=255)
                                      aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGCCAGCAAAAATAGGACGCAATTAGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAg                                                                                                    <  1:718979/143‑1 (MQ=255)
                                        agagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCACTTGGACAATTATCGGGCGCAAAGGCAAACCGGACATTTATATGCCCGGTAAGGTGATATCAAAGCGTTGCTACCCAggc                                                                                                  >  2:705914/1‑141 (MQ=255)
                                        agagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCCGcc                                                                                                  >  1:282635/1‑143 (MQ=255)
                                         gagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGccc                                                                                                 >  2:11876/1‑143 (MQ=255)
                                              cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCCGCAACAATAGGACGCAATTGGACAATAATCGGGCGAAAAGGCAACCCGGGCCTTTATATGCCCGGTAAGTTGTTCTCAAACCGTTCCTATCCAGCCCAtctc                                                                                            >  2:141509/1‑140 (MQ=255)
                                               tcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc                                                                                           <  1:726670/142‑1 (MQ=255)
                                               gcAGTTTAATACAATCCAGTCGCTGCGCCACCAGGAGGCAGAAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc                                                                                           <  2:584785/143‑1 (MQ=255)
                                                   tttAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaa                                                                                       >  1:689361/1‑143 (MQ=255)
                                                              tcatgcGCTGCGCCAACAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGtt                                                                            <  2:96369/143‑1 (MQ=255)
                                                                actcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc                                                                          <  1:190528/141‑1 (MQ=255)
                                                                 ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTAGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACa                                                                         >  2:104401/1‑143 (MQ=255)
                                                                 ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTAGACAATTATCGGGCGAAAAGGCAAACCGGGCAGTTATATGCGCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATGTGCATGAGCGATTCGGTTACa                                                                         >  2:352703/1‑143 (MQ=255)
                                                                    gCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAggg                                                                      >  1:278623/1‑143 (MQ=255)
                                                                     cTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATCGAGTTACGgggg                                                                     >  1:776475/1‑143 (MQ=255)
                                                                          cccccaGGAGGCAGCAAAAATCGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGc                                                                <  2:694094/140‑1 (MQ=255)
                                                                          ccaccaGGAGGCAGCAGAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGc                                                                <  1:48191/143‑1 (MQ=255)
                                                                           caccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCg                                                               >  1:431134/1‑143 (MQ=255)
                                                                              caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTaa                                                            >  2:442345/1‑143 (MQ=255)
                                                                                ggaggCAGCAAAAATAGGACGCGTTTGGAGTGTTGTTGGGTGAAGTGGGGGGTGGGGGGTTTGTGTGCGTGGTCTGTTGTTATCAAAGCGTTGCTATCCGGTGGCTTTGCGTGTACCATTCGGTTACAGGGGCAAGCGTAAAc                                                          <  2:201252/143‑1 (MQ=255)
                                                                                    gcagcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCGGGTAAGTTGTTGTCAAAGCGTTGCTATCCAGCCCCTTTGCATAAACCATTCGGTTCCAGGGCCAAGCGTCAGCTCGa                                                      >  1:201224/1‑143 (MQ=255)
                                                                                       gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTATGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACCATGGCAAGCGTCAACTCGACGc                                                   >  1:260548/1‑143 (MQ=255)
                                                                                       gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACCGGGGCAAGCGTAAACTCGACGc                                                   >  1:783363/1‑143 (MQ=255)
                                                                                               aGGACGCAGTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAg                                           <  1:484590/143‑1 (MQ=255)
                                                                                                      aaTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTAGACGCAGAGCAAGCcgacga                                    >  2:680724/1‑143 (MQ=255)
                                                                                                       aTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCCAGCGTCACCTCGCCGCCGAGCACGCCGACGAg                                   >  1:135117/1‑143 (MQ=255)
                                                                                                               gttATCGGGCGAAAGGGCAAACCGGGCATTTATATGCCCGGTACGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGa                           <  2:436266/142‑1 (MQ=255)
                                                                                                                    cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCCTACGCTTACAGGGGCAAGCGTAAACGCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                      <  2:767462/143‑1 (MQ=255)
                                                                                                                    cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                      <  1:11876/143‑1 (MQ=255)
                                                                                                                    cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGCCAGGACGAgg                      >  2:329449/1‑143 (MQ=255)
                                                                                                                                  aagcgtgCATTCATAGGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAACCCATTCGGTTACAGGGGTAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGc        <  2:282635/137‑1 (MQ=255)
                                                                                                                                   aCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTGACAGGGGCAAGCGGAAACTCGACGCCGAGCAAGCCGACGGGGGGCAGGACGAGGGTGTAAGGCGGCGcc       >  2:492860/1‑143 (MQ=255)
                                                                                                                                     cGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTACACTCGACGCTGAGGATGGGGACCCGATTCAGGACGAGGGAGTAAGGCAGGGCCAt     >  1:143824/1‑143 (MQ=255)
                                                                                                                                      gggCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc    <  2:607097/143‑1 (MQ=255)
                                                                                                                                        tgaTTTTTATGGCCGCTAAGTTGTTATCAAAGTGTTGCTATCCGGCCACTTTGGATAAACTATTCGGTTACAGGGGCAAGCGTTAACTAGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCca  <  2:320542/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCA  >  NC_000913/1233014‑1233292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑