| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,233,157 | T→A | 8.5% | intergenic (‑127/+19) | dsbB ← / ← nhaB | protein thiol:quinone oxidoreductase DsbB/Na(+):H(+) antiporter NhaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,233,157 | 0 | T | A | 8.5% | 126.1 / 4.9 | 47 | intergenic (‑127/+19) | dsbB/nhaB | protein thiol:quinone oxidoreductase DsbB/Na(+):H(+) antiporter NhaB |
| Reads supporting (aligned to +/- strand): ref base T (20/23); new base A (2/2); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-01 | |||||||||||
AAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCA > NC_000913/1233017‑1233292 | aaaagTCGCAACATAATCACTCCCCGGCAATATGCATATAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTatat < 2:493770/143‑1 (MQ=255)aaaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTatat > 1:501221/1‑143 (MQ=255) aaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGcc < 1:680338/143‑1 (MQ=255) aTCGCAACATATGCTTTTCTCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGccc < 2:464983/143‑1 (MQ=255) gCAACCTAATCCTTTCCCTGCAATATGCATAGAGTGCCCAGTTTAAACCAATTCATTCGCTGCGCCACCTGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGt < 1:548220/143‑1 (MQ=255) cAACATAATCATTTCCCTGCAGTAGGCATAGAGGGCGCAGTTTAAACCAATTCATTCGCGGCGCCACCAGGATGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTa < 1:674638/143‑1 (MQ=255) cAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATCTGCCCGGTa > 1:772622/1‑143 (MQ=255) cAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTa > 1:353631/1‑143 (MQ=255) aCATAATCATTGCCCTGCAATTTGCGTCGAGTGCGTAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTAGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtttg < 2:486988/143‑4 (MQ=255) ttaTCCTTTCCCTACAATATGCATAGAGTGCGCGGTTTAAACCAATTCATTCCCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttg < 1:309274/141‑1 (MQ=255) tCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTa < 1:678298/143‑1 (MQ=255) tCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTa < 2:513249/143‑1 (MQ=255) atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAt < 2:778573/143‑1 (MQ=255) cggaTACAGTGCGCAGTTTAAGCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAGATAGGACGCAATTGTACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATc < 2:501504/140‑1 (MQ=255) aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGACAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAtgcgg > 2:266886/1‑139 (MQ=255) aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGCCAGCAAAAATAGGACGCAATTAGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAg < 1:718979/143‑1 (MQ=255) agagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCACTTGGACAATTATCGGGCGCAAAGGCAAACCGGACATTTATATGCCCGGTAAGGTGATATCAAAGCGTTGCTACCCAggc > 2:705914/1‑141 (MQ=255) agagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCCGcc > 1:282635/1‑143 (MQ=255) gagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGccc > 2:11876/1‑143 (MQ=255) cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCCGCAACAATAGGACGCAATTGGACAATAATCGGGCGAAAAGGCAACCCGGGCCTTTATATGCCCGGTAAGTTGTTCTCAAACCGTTCCTATCCAGCCCAtctc > 2:141509/1‑140 (MQ=255) tcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc < 1:726670/142‑1 (MQ=255) gcAGTTTAATACAATCCAGTCGCTGCGCCACCAGGAGGCAGAAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc < 2:584785/143‑1 (MQ=255) tttAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaa > 1:689361/1‑143 (MQ=255) tcatgcGCTGCGCCAACAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGtt < 2:96369/143‑1 (MQ=255) actcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc < 1:190528/141‑1 (MQ=255) ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTAGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACa > 2:104401/1‑143 (MQ=255) ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTAGACAATTATCGGGCGAAAAGGCAAACCGGGCAGTTATATGCGCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATGTGCATGAGCGATTCGGTTACa > 2:352703/1‑143 (MQ=255) gCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAggg > 1:278623/1‑143 (MQ=255) cTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATCGAGTTACGgggg > 1:776475/1‑143 (MQ=255) cccccaGGAGGCAGCAAAAATCGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGc < 2:694094/140‑1 (MQ=255) ccaccaGGAGGCAGCAGAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGc < 1:48191/143‑1 (MQ=255) caccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCg > 1:431134/1‑143 (MQ=255) caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTaa > 2:442345/1‑143 (MQ=255) ggaggCAGCAAAAATAGGACGCGTTTGGAGTGTTGTTGGGTGAAGTGGGGGGTGGGGGGTTTGTGTGCGTGGTCTGTTGTTATCAAAGCGTTGCTATCCGGTGGCTTTGCGTGTACCATTCGGTTACAGGGGCAAGCGTAAAc < 2:201252/143‑1 (MQ=255) gcagcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCGGGTAAGTTGTTGTCAAAGCGTTGCTATCCAGCCCCTTTGCATAAACCATTCGGTTCCAGGGCCAAGCGTCAGCTCGa > 1:201224/1‑143 (MQ=255) gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTATGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACCATGGCAAGCGTCAACTCGACGc > 1:260548/1‑143 (MQ=255) gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACCGGGGCAAGCGTAAACTCGACGc > 1:783363/1‑143 (MQ=255) aGGACGCAGTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAg < 1:484590/143‑1 (MQ=255) aaTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTAGACGCAGAGCAAGCcgacga > 2:680724/1‑143 (MQ=255) aTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCCAGCGTCACCTCGCCGCCGAGCACGCCGACGAg > 1:135117/1‑143 (MQ=255) gttATCGGGCGAAAGGGCAAACCGGGCATTTATATGCCCGGTACGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGa < 2:436266/142‑1 (MQ=255) cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCCTACGCTTACAGGGGCAAGCGTAAACGCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg < 2:767462/143‑1 (MQ=255) cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg < 1:11876/143‑1 (MQ=255) cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGCCAGGACGAgg > 2:329449/1‑143 (MQ=255) aagcgtgCATTCATAGGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAACCCATTCGGTTACAGGGGTAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGc < 2:282635/137‑1 (MQ=255) aCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTGACAGGGGCAAGCGGAAACTCGACGCCGAGCAAGCCGACGGGGGGCAGGACGAGGGTGTAAGGCGGCGcc > 2:492860/1‑143 (MQ=255) cGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTACACTCGACGCTGAGGATGGGGACCCGATTCAGGACGAGGGAGTAAGGCAGGGCCAt > 1:143824/1‑143 (MQ=255) gggCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc < 2:607097/143‑1 (MQ=255) tgaTTTTTATGGCCGCTAAGTTGTTATCAAAGTGTTGCTATCCGGCCACTTTGGATAAACTATTCGGTTACAGGGGCAAGCGTTAACTAGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCca < 2:320542/141‑1 (MQ=255) | AAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCA > NC_000913/1233017‑1233292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |