| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,541,077 | G→C | 14.0% | A505G (GCC→GGC) | narZ ← | nitrate reductase Z subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,541,077 | 0 | G | C | 14.0% | 103.9 / 12.5 | 48 | A505G (GCC→GGC) | narZ | nitrate reductase Z subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (20/21); new base C (5/2); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01 | |||||||||||
GTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTT > NC_000913/1540938‑1541218 | gTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGCGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGCAGGGTTCAAGATGCTGGCGCGGCACGCCGGGAGATTGGCCGCCCCAgggcg > 2:460127/1‑140 (MQ=25) ccATGTGACAGCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTggg < 2:452064/143‑1 (MQ=255) cATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTgggg > 2:397505/1‑143 (MQ=255) cATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTgggg > 1:435139/1‑143 (MQ=255) tgtgATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGgtg > 1:2025/1‑143 (MQ=255) tgtgATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGCCCGGGgtg > 2:574413/1‑143 (MQ=255) tACCAGTGGTTAACACCGGCGCCGAGGATAATCATCGAGCGCCCATGCGTCTTATGGGCGGTATCGGCAAAATCACGGGCGATGGTGTCGATATACTGGCGGGGCACGCCGGCCACTCGCCCACCCCGGGCTGGGGGGTACgg > 2:646972/1‑143 (MQ=255) ccAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGtt < 1:397505/143‑1 (MQ=255) gccgTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGa < 1:471716/140‑1 (MQ=255) gTGGTTAACACCTGCGCCGAGGATAATCATCGTGCGCCTATTCGGTGTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGa < 1:533573/143‑1 (MQ=255) ggtAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGAttt < 1:260126/141‑1 (MQ=255) tGCGCCGAGGATAATCATCGAGCGCCCATGCGCTTTATGGGCGGTATCGGCACATTCCCGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTCCGGTTTGATTTCAGCGTAAt > 1:240529/1‑143 (MQ=255) gcgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGCCTGGGgtg > 1:303792/1‑120 (MQ=255) gcgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGCCTGGGgtg < 2:299168/120‑1 (MQ=255) gcgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGCCTGGGgtg < 2:303792/120‑1 (MQ=255) gcgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGCCTGGGgtg > 1:299168/1‑120 (MQ=255) tcatcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCACATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTCATCTTTCGCACTGttt > 2:535663/1‑143 (MQ=255) tcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCa > 1:659660/1‑143 (MQ=255) cacgcATGCGGTTTACGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTc < 1:92773/139‑1 (MQ=255) gcCCATGCGTTTTATGGGCGGTATCGTCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCCCCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTcc > 1:431899/1‑143 (MQ=255) gcCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATCTACTGGCGCGGCACGCCGGTAATTTGCTCGCCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTcc < 1:574413/143‑1 (MQ=255) aTGCGTCTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGcc < 1:438435/143‑1 (MQ=255) gTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGGTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTAGTCTTCCAGCCcgcg > 1:165162/1‑143 (MQ=255) tttGTGGGCGGTATCGGCAAGTTCGCGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGAt < 2:175176/143‑1 (MQ=255) tctGGGCGGTACCGGCAAATTCACGGGCGGTGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCCCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCg < 2:425861/141‑1 (MQ=255) gcgtgaTCGGCAAATTCACGGGAGCTGGTTTCAATCTACTGGCGCGGCACGGCGATCATTTGCTTAGCCCTGGCTGGTGTGTACGGTTTGATTTCAGCGTCATCTGTGCCACTGTTTTCATCTTCCAGCCCGCGATCGAGGcc < 2:385780/138‑1 (MQ=255) cGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTATTCTTTCGCACGGGTCTCATCTTCCAGCCCGCGATCGAGGCCg > 1:741491/1‑143 (MQ=255) gtctCGGCAAATGCGCGGGCGATGGTTTCAATCTACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTCTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTa < 2:326067/140‑1 (MQ=255) tCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAAtt > 1:630254/1‑143 (MQ=255) ggCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCg > 1:484057/1‑143 (MQ=255) aaTTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAg < 1:241190/143‑1 (MQ=255) ttCACGGGCGATGGTTTCAATATACTGGCGCGGCCCGCCGGTAATCCGCTCACCCCAGGCTGCGGTGCACGGTTTGATTCCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTa < 2:599422/143‑1 (MQ=255) gttgctttCAATATACTCGCGCGGCACGCCGGTAATTTGCTCCCCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAACCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCAt < 1:466264/138‑1 (MQ=255) ggTTTCAATAGACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATaaa < 2:657002/143‑1 (MQ=255) gTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAAc > 1:677083/1‑143 (MQ=255) ttCGATATACTTGCGCGGCTCGTCGGTACTTGGCTCACCTCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAAGCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGc < 2:526481/143‑1 (MQ=255) attgtgcTGGCGCGGCACGCCGGTAATTTGCTTACCCCAGGCTGGGGTGTACGGTTTGATTGCAGCGTAATCTTTCGCACTGTTGTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGa < 2:233657/137‑1 (MQ=255) atatACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGac > 1:715655/1‑143 (MQ=255) cgGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGCACGAGACGTTATTTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAgg > 1:330444/1‑143 (MQ=255) cgGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACAGATTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGCCAgg > 1:331427/1‑143 (MQ=255) aCGCCGGTAATTTGCTCCCCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGtt < 2:431899/143‑1 (MQ=255) ccGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGCTTTCATCTTCCAGCCCGCGATCGAGGCCGTCATTCGCCAGTACCAAGTCATCAACGCCGACCaccggaccggggcggcc > 2:296913/1‑136 (MQ=255) aaccgtacACCCCAGCCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGAc > 1:404319/8‑143 (MQ=255) gCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAAc < 2:240529/143‑1 (MQ=255) tCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTGGCCATCGAACAACGt > 2:256134/1‑143 (MQ=255) cACCCCAGCCTGGGGTGTACGGTTTGATTTCAGCGTGATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACCGGTGTTGCCATCGACTAACGTc > 1:206467/1‑143 (MQ=255) ccccAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCCCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAg < 2:609160/143‑1 (MQ=255) cccAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCAACGGACAGGTGTTGCCATCGACTACCGTCAgg > 1:712236/1‑143 (MQ=255) cccAGGCTGGGGTGTACGGTTTGATTTCAGCGGAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGGCAgg > 1:385777/1‑143 (MQ=255) ggCTGGGGTGTACGGTTTGATGTCAGCGGAATCGTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTCATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGtttt < 2:177216/143‑1 (MQ=255) | GTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTT > NC_000913/1540938‑1541218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |