| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,642,373 | C→G | 11.9% | intergenic (+162/+116) | ynfS → / ← ydfT | Qin prophage; protein YnfS/Qin prophage; putative antitermination protein Q |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,642,373 | 0 | C | G | 11.9% | 119.2 / 9.8 | 42 | intergenic (+162/+116) | ynfS/ydfT | Qin prophage; protein YnfS/Qin prophage; putative antitermination protein Q |
| Reads supporting (aligned to +/- strand): ref base C (21/16); new base G (3/2); total (24/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01 | |||||||||||
GGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCG > NC_000913/1642233‑1642514 | gggACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAg > 1:101375/1‑143 (MQ=255) aCATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCgg > 1:544617/1‑143 (MQ=255) aCATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCgg < 2:44374/143‑1 (MQ=255) aCATAGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCTGCGCGTAACCGTAGTTGTAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCgg < 2:166080/143‑1 (MQ=255) tGTAATGGAAGGTAACATTTGAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACACACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttt < 2:583014/143‑1 (MQ=255) aCTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttgt < 1:280979/143‑3 (MQ=255) cTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTa < 2:705901/143‑1 (MQ=255) aaaaCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTc > 1:734167/1‑143 (MQ=255) aaaaCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTc < 1:435688/143‑1 (MQ=255) ttAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAACCGGTTTTCTACTTTTTCTTAACGTc > 2:368122/1‑143 (MQ=255) tAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAACACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCg < 2:133210/143‑1 (MQ=255) aGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACa > 1:750138/1‑143 (MQ=255) aGGCAAATAACCAAGTCACCAGCTAAATAAATAGTTAACAGACAGCAGTCACGGGATGAGATTCAACATTACCATTGCCCCAGTTAAAGCACAAAACCCGCTCATCAGCGGGTTGTCTACTTTTTCTTAACGTCGGGTATACa > 2:730406/1‑143 (MQ=255) aaCCAAGTCACCAGCTAAATAATAAGTTAACAGACATTAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCGCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATTCAGAGCCCAt > 2:201645/1‑143 (MQ=255) cAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGt > 2:363166/1‑143 (MQ=255) aaGTCACCAGCTAAAGAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGtt < 2:464448/143‑1 (MQ=255) cAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaaaa < 2:620176/143‑1 (MQ=255) aataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTACCGTCGGGGATACAAAGCCCATAGTCGCAAACACTTTCTCCa > 2:520195/1‑143 (MQ=255) ataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGCGTTTTCTACTTTTTCTTAACGTCGCGCATACACAGCCCATCGTCGAAACAAtttcaactac > 2:770662/1‑136 (MQ=255) aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATCtttttt > 1:761198/1‑143 (MQ=255) aTGAGTCCCGGGAGGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAt < 1:86767/143‑1 (MQ=255) tCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCCTCGTTGAAAAAAATTTATCCATATTTTTTGAACAACGCaaa > 2:701329/1‑143 (MQ=255) gcccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCaaa < 1:625601/142‑1 (MQ=255) cGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcat > 1:234852/1‑143 (MQ=255) gggATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatc < 1:156212/143‑1 (MQ=255) aaCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCt < 1:244237/143‑1 (MQ=255) aCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCtt < 1:542152/143‑1 (MQ=255) aTTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATAAAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGCCGCAATATTCa > 2:647/1‑143 (MQ=255) tACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGc < 2:251519/143‑1 (MQ=255) aTTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTCTAAAAAAATGCAAACATCATGTCGCCATCTTCAGCaaca > 1:742849/1‑141 (MQ=255) ttGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATACTTTTTTAAAAATGCAAACATCATGTCGCCATCTTCAGCaaaaa < 2:169293/143‑1 (MQ=255) ccccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAACATCa > 2:770604/1‑143 (MQ=255) cccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCAt > 1:637369/1‑143 (MQ=255) ccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCAtt > 1:467449/1‑143 (MQ=255) tttAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTGAAAGCTGGTATACAAAGCCCATCGTTGAAAAAATTTTCTCCATATTTTTTGAAAAATGCAAACATCATGTCGCCACTCTCAGCAACAATCATTTAt > 2:168527/1‑143 (MQ=255) ttAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTAtc < 2:362039/143‑1 (MQ=255) aaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCtt > 1:580889/1‑143 (MQ=255) aaaCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATTGTTGTATAAATTTTATCCATATTTTTTGAAACATGCAAACATCATGTTGCCATCCTCAGCAACAATCATTTATCTCGTCACCtt > 2:727260/1‑143 (MQ=255) aaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTc > 1:639400/1‑143 (MQ=255) aaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTc > 1:632326/1‑143 (MQ=255) aCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTcc < 1:730406/143‑1 (MQ=255) gCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGCAAAAATTTTATCAATATTTTTTGAAAAATGCAAACATCATGTCGCCGTCTTCAGGCGAAATCATTTATCACGTCACCTTTCTCaa > 1:295400/1‑143 (MQ=255) tcagcaGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAAtt < 2:751254/143‑1 (MQ=255) tcaGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTgcg > 1:472159/1‑143 (MQ=255) | GGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCG > NC_000913/1642233‑1642514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |